Hairpin-bisulfite sequencing of cells exposed to decitabine documents the process of DNA demethylation
Although the mechanism of DNA demethylating drugs has been understood for many years, the direct effect of these drugs on methylation of the complementary strands of DNA has not been formally demonstrated. By using hairpin-bisulphite sequencing, we describe the kinetics and pattern of DNA methylatio...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2021-11-01
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| Series: | Epigenetics |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592294.2020.1861169 |
| _version_ | 1797678800886038528 |
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| author | Issam M Mayyas Robert J Weeks Robert C Day Helena E Magrath Karina M O’Connor Olga Kardailsky Timothy A Hore Mark B Hampton Ian M Morison |
| author_facet | Issam M Mayyas Robert J Weeks Robert C Day Helena E Magrath Karina M O’Connor Olga Kardailsky Timothy A Hore Mark B Hampton Ian M Morison |
| author_sort | Issam M Mayyas |
| collection | DOAJ |
| description | Although the mechanism of DNA demethylating drugs has been understood for many years, the direct effect of these drugs on methylation of the complementary strands of DNA has not been formally demonstrated. By using hairpin-bisulphite sequencing, we describe the kinetics and pattern of DNA methylation following treatment of cells by the DNA methyltransferase 1 (DNMT1) inhibitor, decitabine. As expected, we demonstrate complete loss of methylation on the daughter strand following S-phase in selected densely methylated genes in synchronized Jurkat cells. Thereafter, cells showed a heterogeneous pattern of methylation reflecting replication of the unmethylated strand and restoration of methylation. |
| first_indexed | 2024-03-11T23:05:12Z |
| format | Article |
| id | doaj.art-1c6526df08f64f94b446cbb0b8761e4c |
| institution | Directory Open Access Journal |
| issn | 1559-2294 1559-2308 |
| language | English |
| last_indexed | 2024-03-11T23:05:12Z |
| publishDate | 2021-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj.art-1c6526df08f64f94b446cbb0b8761e4c2023-09-21T13:09:25ZengTaylor & Francis GroupEpigenetics1559-22941559-23082021-11-0116111251125910.1080/15592294.2020.18611691861169Hairpin-bisulfite sequencing of cells exposed to decitabine documents the process of DNA demethylationIssam M Mayyas0Robert J Weeks1Robert C Day2Helena E Magrath3Karina M O’Connor4Olga Kardailsky5Timothy A Hore6Mark B Hampton7Ian M Morison8University of OtagoUniversity of OtagoUniversity of OtagoUniversity of OtagoUniversity of OtagoUniversity of OtagoUniversity of OtagoUniversity of OtagoUniversity of OtagoAlthough the mechanism of DNA demethylating drugs has been understood for many years, the direct effect of these drugs on methylation of the complementary strands of DNA has not been formally demonstrated. By using hairpin-bisulphite sequencing, we describe the kinetics and pattern of DNA methylation following treatment of cells by the DNA methyltransferase 1 (DNMT1) inhibitor, decitabine. As expected, we demonstrate complete loss of methylation on the daughter strand following S-phase in selected densely methylated genes in synchronized Jurkat cells. Thereafter, cells showed a heterogeneous pattern of methylation reflecting replication of the unmethylated strand and restoration of methylation.http://dx.doi.org/10.1080/15592294.2020.1861169demethylationdecitabinednmt1 inhibition |
| spellingShingle | Issam M Mayyas Robert J Weeks Robert C Day Helena E Magrath Karina M O’Connor Olga Kardailsky Timothy A Hore Mark B Hampton Ian M Morison Hairpin-bisulfite sequencing of cells exposed to decitabine documents the process of DNA demethylation Epigenetics demethylation decitabine dnmt1 inhibition |
| title | Hairpin-bisulfite sequencing of cells exposed to decitabine documents the process of DNA demethylation |
| title_full | Hairpin-bisulfite sequencing of cells exposed to decitabine documents the process of DNA demethylation |
| title_fullStr | Hairpin-bisulfite sequencing of cells exposed to decitabine documents the process of DNA demethylation |
| title_full_unstemmed | Hairpin-bisulfite sequencing of cells exposed to decitabine documents the process of DNA demethylation |
| title_short | Hairpin-bisulfite sequencing of cells exposed to decitabine documents the process of DNA demethylation |
| title_sort | hairpin bisulfite sequencing of cells exposed to decitabine documents the process of dna demethylation |
| topic | demethylation decitabine dnmt1 inhibition |
| url | http://dx.doi.org/10.1080/15592294.2020.1861169 |
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