Chemical constituents, antibacterial and antioxidant properties of the essential oil flower of Tagetes minuta grown in Cala community Eastern Cape, South Africa
Abstract Background Tagetes minuta has a long record of human use for the treatment of stomach and intestinal diseases. Most drugs used for diseases treatment are less efficacious with side effects and this brought the search for new treatment regimens mainly from medicinal plants. Method The essent...
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| Format: | Article |
| Language: | English |
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BMC
2017-07-01
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| Series: | BMC Complementary and Alternative Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12906-017-1861-6 |
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| author | Aboi Igwaran Benson Chucks Iweriebor Sunday Ofuzim Okoh Uchechukwu Uchechukwu Nwodo Larry Chikwelu Obi Anthony Ifeanyi Okoh |
| author_facet | Aboi Igwaran Benson Chucks Iweriebor Sunday Ofuzim Okoh Uchechukwu Uchechukwu Nwodo Larry Chikwelu Obi Anthony Ifeanyi Okoh |
| author_sort | Aboi Igwaran |
| collection | DOAJ |
| description | Abstract Background Tagetes minuta has a long record of human use for the treatment of stomach and intestinal diseases. Most drugs used for diseases treatment are less efficacious with side effects and this brought the search for new treatment regimens mainly from medicinal plants. Method The essential oil (EO) was extracted by Clevenger’s-type apparatus and its chemical composition, antioxidant and antibacterial properties were determined by GC-MS, spectrophotometric and broth dilution methods respectively. S. uberis, E. cloacae, S. aureus, M. smegmatis, L. ivanovii, Vibrio spp. and E. coli bacteria strains were used as test bacteria. Results GC-MS analysis revealed 98 compounds in the EO flower of T. minuta and β-Ocimene (14. 40%) was the major chemical constituents. The EO exhibited highest inhibitory effect against DPPH radical, followed by its effect on ABTS, while LP radical showed the least sensitivity with IC50 values of 2.45 mg/mL, 2.76 mg/mL and 3.23 mg/mL respectively. The EO showed antibacterial activities against all test organisms with MIC value for S. aureus, M. smegatis and S. uberis at 0.125 mg/mL and for L. ivanovii, Vibrio spp., E. cloacae and E. coli at 0.06 mg/mL. The EO showed MBC against E. cloacae and E. coli at 0.06 mg/mL at 0.5 mg/mL for S. uberis and 0.125 mg/mL for Vibrio spp. Conclusion Findings from this study suggest that the EO of T. minuta flower may be a useful candidate in the search for lead constituents for the synthesis of new potent antibacterial and antioxidant agent. |
| first_indexed | 2024-12-22T17:16:46Z |
| format | Article |
| id | doaj.art-1c665fef2591477587ccbdd5f9f13fb7 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-22T17:16:46Z |
| publishDate | 2017-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-1c665fef2591477587ccbdd5f9f13fb72022-12-21T18:18:56ZengBMCBMC Complementary and Alternative Medicine1472-68822017-07-0117111010.1186/s12906-017-1861-6Chemical constituents, antibacterial and antioxidant properties of the essential oil flower of Tagetes minuta grown in Cala community Eastern Cape, South AfricaAboi Igwaran0Benson Chucks Iweriebor1Sunday Ofuzim Okoh2Uchechukwu Uchechukwu Nwodo3Larry Chikwelu Obi4Anthony Ifeanyi Okoh5SAMRC Microbial Water Quality Monitoring Centre, University of Fort HareSAMRC Microbial Water Quality Monitoring Centre, University of Fort HareApplied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort HareSAMRC Microbial Water Quality Monitoring Centre, University of Fort HareAcademic and Research Division, University of Fort HareSAMRC Microbial Water Quality Monitoring Centre, University of Fort HareAbstract Background Tagetes minuta has a long record of human use for the treatment of stomach and intestinal diseases. Most drugs used for diseases treatment are less efficacious with side effects and this brought the search for new treatment regimens mainly from medicinal plants. Method The essential oil (EO) was extracted by Clevenger’s-type apparatus and its chemical composition, antioxidant and antibacterial properties were determined by GC-MS, spectrophotometric and broth dilution methods respectively. S. uberis, E. cloacae, S. aureus, M. smegmatis, L. ivanovii, Vibrio spp. and E. coli bacteria strains were used as test bacteria. Results GC-MS analysis revealed 98 compounds in the EO flower of T. minuta and β-Ocimene (14. 40%) was the major chemical constituents. The EO exhibited highest inhibitory effect against DPPH radical, followed by its effect on ABTS, while LP radical showed the least sensitivity with IC50 values of 2.45 mg/mL, 2.76 mg/mL and 3.23 mg/mL respectively. The EO showed antibacterial activities against all test organisms with MIC value for S. aureus, M. smegatis and S. uberis at 0.125 mg/mL and for L. ivanovii, Vibrio spp., E. cloacae and E. coli at 0.06 mg/mL. The EO showed MBC against E. cloacae and E. coli at 0.06 mg/mL at 0.5 mg/mL for S. uberis and 0.125 mg/mL for Vibrio spp. Conclusion Findings from this study suggest that the EO of T. minuta flower may be a useful candidate in the search for lead constituents for the synthesis of new potent antibacterial and antioxidant agent.http://link.springer.com/article/10.1186/s12906-017-1861-6AntibacterialAntioxidantTegates minutaVitamin C |
| spellingShingle | Aboi Igwaran Benson Chucks Iweriebor Sunday Ofuzim Okoh Uchechukwu Uchechukwu Nwodo Larry Chikwelu Obi Anthony Ifeanyi Okoh Chemical constituents, antibacterial and antioxidant properties of the essential oil flower of Tagetes minuta grown in Cala community Eastern Cape, South Africa BMC Complementary and Alternative Medicine Antibacterial Antioxidant Tegates minuta Vitamin C |
| title | Chemical constituents, antibacterial and antioxidant properties of the essential oil flower of Tagetes minuta grown in Cala community Eastern Cape, South Africa |
| title_full | Chemical constituents, antibacterial and antioxidant properties of the essential oil flower of Tagetes minuta grown in Cala community Eastern Cape, South Africa |
| title_fullStr | Chemical constituents, antibacterial and antioxidant properties of the essential oil flower of Tagetes minuta grown in Cala community Eastern Cape, South Africa |
| title_full_unstemmed | Chemical constituents, antibacterial and antioxidant properties of the essential oil flower of Tagetes minuta grown in Cala community Eastern Cape, South Africa |
| title_short | Chemical constituents, antibacterial and antioxidant properties of the essential oil flower of Tagetes minuta grown in Cala community Eastern Cape, South Africa |
| title_sort | chemical constituents antibacterial and antioxidant properties of the essential oil flower of tagetes minuta grown in cala community eastern cape south africa |
| topic | Antibacterial Antioxidant Tegates minuta Vitamin C |
| url | http://link.springer.com/article/10.1186/s12906-017-1861-6 |
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