Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics
An endophytic strain of <i>Streptomyces antimycoticus</i> L-1 was isolated from healthy medicinal plant leaves of <i>Mentha longifolia</i> L. and used for the green synthesis of silver nanoparticles (Ag-NPs), through the use of secreted enzymes and proteins. UV–vis spectrosco...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-10-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/10/10/2082 |
| _version_ | 1797550209995112448 |
|---|---|
| author | Salem S. Salem Ehab F. EL-Belely Gniewko Niedbała Maryam M. Alnoman Saad El-Din Hassan Ahmed Mohamed Eid Tharwat I. Shaheen Amr Elkelish Amr Fouda |
| author_facet | Salem S. Salem Ehab F. EL-Belely Gniewko Niedbała Maryam M. Alnoman Saad El-Din Hassan Ahmed Mohamed Eid Tharwat I. Shaheen Amr Elkelish Amr Fouda |
| author_sort | Salem S. Salem |
| collection | DOAJ |
| description | An endophytic strain of <i>Streptomyces antimycoticus</i> L-1 was isolated from healthy medicinal plant leaves of <i>Mentha longifolia</i> L. and used for the green synthesis of silver nanoparticles (Ag-NPs), through the use of secreted enzymes and proteins. UV–vis spectroscopy, Fourier-transform infrared (FT-IR), transmission electron microscopy (TEM), X-ray diffraction (XRD), and dynamic light scattering (DLS) analyses of the Ag-NPs were carried out. The XRD, TEM, and FT-IR analysis results demonstrated the successful biosynthesis of crystalline, spherical Ag-NPs with a particle size of 13–40 nm. Further, the stability of the Ag-NPs was assessed by detecting the surface Plasmon resonance (SPR) at 415 nm for one month or by measuring the NPs surface charge (−19.2 mV) by zeta potential analysis (ζ). The green-synthesized Ag-NPs exhibited broad-spectrum antibacterial activity at different concentrations (6.25–100 ppm) against the pathogens <i>Staphylococcus aureus, Bacillus subtilis Pseudomonas aeruginosa, Escherichia coli,</i> and <i>Salmonella typhimurium</i> with a clear inhibition zone ranging from (9.5 ± 0.4) nm to (21.7 ± 1.0) mm. Furthermore, the green-synthesized Ag-NPs displayed high efficacy against the Caco-2 cancerous cell line (the half maximal inhibitory concentration (IC<sub>50</sub>) = 5.7 ± 0.2 ppm). With respect to antibacterial and in-vitro cytotoxicity analyses, the Ag-NPs concentration of 100 ppm was selected as a safe dose for loading onto cotton fabrics. The scanning electron microscopy connected with energy-dispersive X-ray spectroscopy (SEM-EDX) for the nano-finished fabrics showed the distribution of Ag-NPs as 2% of the total fabric elements. Moreover, the nano-finished fabrics exhibited more activity against pathogenic Gram-positive and Gram-negative bacteria, even after 10 washing cycles, indicating the stability of the treated fabrics. |
| first_indexed | 2024-03-10T15:26:17Z |
| format | Article |
| id | doaj.art-1c66c8ebe2244feda62b40265b713b0f |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T15:26:17Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-1c66c8ebe2244feda62b40265b713b0f2023-11-20T17:59:29ZengMDPI AGNanomaterials2079-49912020-10-011010208210.3390/nano10102082Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile FabricsSalem S. Salem0Ehab F. EL-Belely1Gniewko Niedbała2Maryam M. Alnoman3Saad El-Din Hassan4Ahmed Mohamed Eid5Tharwat I. Shaheen6Amr Elkelish7Amr Fouda8Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptDepartment of Biosystems Engineering, Faculty of Environmental Engineering and Mechanical Engineering, Poznań University of Life Sciences, Wojska Polskiego 50, 60-627 Poznań, PolandBiology Department, Faculty of Science, Taibah University, Al-Sharm, Yanbu El-Bahr 46429, Saudi ArabiaDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptNational Research Centre, El-Behouth St., Dokki, Giza 12622, EgyptBotany Department, Faculty of Science, Suez Canal University, Ismailia 41522, EgyptDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptAn endophytic strain of <i>Streptomyces antimycoticus</i> L-1 was isolated from healthy medicinal plant leaves of <i>Mentha longifolia</i> L. and used for the green synthesis of silver nanoparticles (Ag-NPs), through the use of secreted enzymes and proteins. UV–vis spectroscopy, Fourier-transform infrared (FT-IR), transmission electron microscopy (TEM), X-ray diffraction (XRD), and dynamic light scattering (DLS) analyses of the Ag-NPs were carried out. The XRD, TEM, and FT-IR analysis results demonstrated the successful biosynthesis of crystalline, spherical Ag-NPs with a particle size of 13–40 nm. Further, the stability of the Ag-NPs was assessed by detecting the surface Plasmon resonance (SPR) at 415 nm for one month or by measuring the NPs surface charge (−19.2 mV) by zeta potential analysis (ζ). The green-synthesized Ag-NPs exhibited broad-spectrum antibacterial activity at different concentrations (6.25–100 ppm) against the pathogens <i>Staphylococcus aureus, Bacillus subtilis Pseudomonas aeruginosa, Escherichia coli,</i> and <i>Salmonella typhimurium</i> with a clear inhibition zone ranging from (9.5 ± 0.4) nm to (21.7 ± 1.0) mm. Furthermore, the green-synthesized Ag-NPs displayed high efficacy against the Caco-2 cancerous cell line (the half maximal inhibitory concentration (IC<sub>50</sub>) = 5.7 ± 0.2 ppm). With respect to antibacterial and in-vitro cytotoxicity analyses, the Ag-NPs concentration of 100 ppm was selected as a safe dose for loading onto cotton fabrics. The scanning electron microscopy connected with energy-dispersive X-ray spectroscopy (SEM-EDX) for the nano-finished fabrics showed the distribution of Ag-NPs as 2% of the total fabric elements. Moreover, the nano-finished fabrics exhibited more activity against pathogenic Gram-positive and Gram-negative bacteria, even after 10 washing cycles, indicating the stability of the treated fabrics.https://www.mdpi.com/2079-4991/10/10/2082endophytic actinomycetesAg-NPsgreen synthesisstreptomyces spp.cotton fabricsantibacterial and cytotoxicity activities |
| spellingShingle | Salem S. Salem Ehab F. EL-Belely Gniewko Niedbała Maryam M. Alnoman Saad El-Din Hassan Ahmed Mohamed Eid Tharwat I. Shaheen Amr Elkelish Amr Fouda Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics Nanomaterials endophytic actinomycetes Ag-NPs green synthesis streptomyces spp. cotton fabrics antibacterial and cytotoxicity activities |
| title | Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics |
| title_full | Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics |
| title_fullStr | Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics |
| title_full_unstemmed | Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics |
| title_short | Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics |
| title_sort | bactericidal and in vitro cytotoxic efficacy of silver nanoparticles ag nps fabricated by endophytic actinomycetes and their use as coating for the textile fabrics |
| topic | endophytic actinomycetes Ag-NPs green synthesis streptomyces spp. cotton fabrics antibacterial and cytotoxicity activities |
| url | https://www.mdpi.com/2079-4991/10/10/2082 |
| work_keys_str_mv | AT salemssalem bactericidalandinvitrocytotoxicefficacyofsilvernanoparticlesagnpsfabricatedbyendophyticactinomycetesandtheiruseascoatingforthetextilefabrics AT ehabfelbelely bactericidalandinvitrocytotoxicefficacyofsilvernanoparticlesagnpsfabricatedbyendophyticactinomycetesandtheiruseascoatingforthetextilefabrics AT gniewkoniedbała bactericidalandinvitrocytotoxicefficacyofsilvernanoparticlesagnpsfabricatedbyendophyticactinomycetesandtheiruseascoatingforthetextilefabrics AT maryammalnoman bactericidalandinvitrocytotoxicefficacyofsilvernanoparticlesagnpsfabricatedbyendophyticactinomycetesandtheiruseascoatingforthetextilefabrics AT saadeldinhassan bactericidalandinvitrocytotoxicefficacyofsilvernanoparticlesagnpsfabricatedbyendophyticactinomycetesandtheiruseascoatingforthetextilefabrics AT ahmedmohamedeid bactericidalandinvitrocytotoxicefficacyofsilvernanoparticlesagnpsfabricatedbyendophyticactinomycetesandtheiruseascoatingforthetextilefabrics AT tharwatishaheen bactericidalandinvitrocytotoxicefficacyofsilvernanoparticlesagnpsfabricatedbyendophyticactinomycetesandtheiruseascoatingforthetextilefabrics AT amrelkelish bactericidalandinvitrocytotoxicefficacyofsilvernanoparticlesagnpsfabricatedbyendophyticactinomycetesandtheiruseascoatingforthetextilefabrics AT amrfouda bactericidalandinvitrocytotoxicefficacyofsilvernanoparticlesagnpsfabricatedbyendophyticactinomycetesandtheiruseascoatingforthetextilefabrics |