Phytochemical Profile, Antioxidant Activity, and Cytotoxicity Assessment of <i>Tagetes erecta</i> L. Flowers
<i>Tagetes erecta</i> L. is a popular ornamental plant of the Asteraceae family, which is widely cultivated not only for its decorative use, but also for the extraction of lutein. Besides carotenoid representatives, which have been extensively studied, other important classes of secondar...
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MDPI AG
2021-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/5/1201 |
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| author | Ana Flavia Burlec Łukasz Pecio Solomiia Kozachok Cornelia Mircea Andreia Corciovă Liliana Vereștiuc Oana Cioancă Wiesław Oleszek Monica Hăncianu |
| author_facet | Ana Flavia Burlec Łukasz Pecio Solomiia Kozachok Cornelia Mircea Andreia Corciovă Liliana Vereștiuc Oana Cioancă Wiesław Oleszek Monica Hăncianu |
| author_sort | Ana Flavia Burlec |
| collection | DOAJ |
| description | <i>Tagetes erecta</i> L. is a popular ornamental plant of the Asteraceae family, which is widely cultivated not only for its decorative use, but also for the extraction of lutein. Besides carotenoid representatives, which have been extensively studied, other important classes of secondary metabolites present in the plant, such as polyphenols, could exhibit important biological activities. The phytochemical analysis of a methanolic extract obtained from <i>T. erecta</i> inflorescences was achieved using liquid chromatography–mass spectrometry (LC-MS) techniques. The extract was further subjected to a multistep purification process, which allowed the separation of different fractions. The total extract and its fractions contain several polyphenolic compounds, such as hydroxybenzoic and hydroxycinnamic acid derivatives, flavonols (especially quercetagetin glycosides), and several aglycons (e.g., quercetin, patuletin). One of the fractions, containing mostly quercetagitrin, was subjected to two different antioxidant assays (metal chelating activity and lipoxygenase inhibition) and to in vitro cytotoxicity assessment. Generally, the biological assays showed promising results for the investigated fraction compared to the initial extract. Given the encouraging outcome of the in vitro assays, further purification and structural analysis of compounds from <i>T. erecta</i> extracts, as well as further in vivo investigations are justified. |
| first_indexed | 2024-03-09T00:35:13Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T00:35:13Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1c6a3828e9b549d1956869a99854d94c2023-12-11T18:12:33ZengMDPI AGMolecules1420-30492021-02-01265120110.3390/molecules26051201Phytochemical Profile, Antioxidant Activity, and Cytotoxicity Assessment of <i>Tagetes erecta</i> L. FlowersAna Flavia Burlec0Łukasz Pecio1Solomiia Kozachok2Cornelia Mircea3Andreia Corciovă4Liliana Vereștiuc5Oana Cioancă6Wiesław Oleszek7Monica Hăncianu8Department of Drug Analysis, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Street, 700115 Iasi, RomaniaDepartment of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation—State Research Institute, Czartoryskich 8, 24-100 Puławy, PolandDepartment of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation—State Research Institute, Czartoryskich 8, 24-100 Puławy, PolandDepartment of Pharmaceutical Biochemistry and Clinical Laboratory, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Street, 700115 Iasi, RomaniaDepartment of Drug Analysis, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Street, 700115 Iasi, RomaniaDepartment of Biomedical Sciences, Faculty of Medical Bioengineering, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Street, 700115 Iasi, RomaniaDepartment of Pharmacognosy, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Street, 700115 Iasi, RomaniaDepartment of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation—State Research Institute, Czartoryskich 8, 24-100 Puławy, PolandDepartment of Pharmacognosy, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Street, 700115 Iasi, Romania<i>Tagetes erecta</i> L. is a popular ornamental plant of the Asteraceae family, which is widely cultivated not only for its decorative use, but also for the extraction of lutein. Besides carotenoid representatives, which have been extensively studied, other important classes of secondary metabolites present in the plant, such as polyphenols, could exhibit important biological activities. The phytochemical analysis of a methanolic extract obtained from <i>T. erecta</i> inflorescences was achieved using liquid chromatography–mass spectrometry (LC-MS) techniques. The extract was further subjected to a multistep purification process, which allowed the separation of different fractions. The total extract and its fractions contain several polyphenolic compounds, such as hydroxybenzoic and hydroxycinnamic acid derivatives, flavonols (especially quercetagetin glycosides), and several aglycons (e.g., quercetin, patuletin). One of the fractions, containing mostly quercetagitrin, was subjected to two different antioxidant assays (metal chelating activity and lipoxygenase inhibition) and to in vitro cytotoxicity assessment. Generally, the biological assays showed promising results for the investigated fraction compared to the initial extract. Given the encouraging outcome of the in vitro assays, further purification and structural analysis of compounds from <i>T. erecta</i> extracts, as well as further in vivo investigations are justified.https://www.mdpi.com/1420-3049/26/5/1201<i>Tagetes erecta</i> L.AsteraceaeHR-QTOF/MSpolyphenolsquercetagitrinlipoxygenase |
| spellingShingle | Ana Flavia Burlec Łukasz Pecio Solomiia Kozachok Cornelia Mircea Andreia Corciovă Liliana Vereștiuc Oana Cioancă Wiesław Oleszek Monica Hăncianu Phytochemical Profile, Antioxidant Activity, and Cytotoxicity Assessment of <i>Tagetes erecta</i> L. Flowers Molecules <i>Tagetes erecta</i> L. Asteraceae HR-QTOF/MS polyphenols quercetagitrin lipoxygenase |
| title | Phytochemical Profile, Antioxidant Activity, and Cytotoxicity Assessment of <i>Tagetes erecta</i> L. Flowers |
| title_full | Phytochemical Profile, Antioxidant Activity, and Cytotoxicity Assessment of <i>Tagetes erecta</i> L. Flowers |
| title_fullStr | Phytochemical Profile, Antioxidant Activity, and Cytotoxicity Assessment of <i>Tagetes erecta</i> L. Flowers |
| title_full_unstemmed | Phytochemical Profile, Antioxidant Activity, and Cytotoxicity Assessment of <i>Tagetes erecta</i> L. Flowers |
| title_short | Phytochemical Profile, Antioxidant Activity, and Cytotoxicity Assessment of <i>Tagetes erecta</i> L. Flowers |
| title_sort | phytochemical profile antioxidant activity and cytotoxicity assessment of i tagetes erecta i l flowers |
| topic | <i>Tagetes erecta</i> L. Asteraceae HR-QTOF/MS polyphenols quercetagitrin lipoxygenase |
| url | https://www.mdpi.com/1420-3049/26/5/1201 |
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