Transcriptome Analysis of Cisplatin, Cannabidiol, and Intermittent Serum Starvation Alone and in Various Combinations on Colorectal Cancer Cells
Platinum-derived chemotherapy medications are often combined with other conventional therapies for treating different tumors, including colorectal cancer. However, the development of drug resistance and multiple adverse effects remain common in clinical settings. Thus, there is a necessity to find n...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/19/14743 |
| _version_ | 1797447289129664512 |
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| author | Viktoriia Cherkasova Yaroslav Ilnytskyy Olga Kovalchuk Igor Kovalchuk |
| author_facet | Viktoriia Cherkasova Yaroslav Ilnytskyy Olga Kovalchuk Igor Kovalchuk |
| author_sort | Viktoriia Cherkasova |
| collection | DOAJ |
| description | Platinum-derived chemotherapy medications are often combined with other conventional therapies for treating different tumors, including colorectal cancer. However, the development of drug resistance and multiple adverse effects remain common in clinical settings. Thus, there is a necessity to find novel treatments and drug combinations that could effectively target colorectal cancer cells and lower the probability of disease relapse. To find potential synergistic interaction, we designed multiple different combinations between cisplatin, cannabidiol, and intermittent serum starvation on colorectal cancer cell lines. Based on the cell viability assay, we found that combinations between cannabidiol and intermittent serum starvation, cisplatin and intermittent serum starvation, as well as cisplatin, cannabidiol, and intermittent serum starvation can work in a synergistic fashion on different colorectal cancer cell lines. Furthermore, we analyzed differentially expressed genes and affected pathways in colorectal cancer cell lines to understand further the potential molecular mechanisms behind the treatments and their interactions. We found that synergistic interaction between cannabidiol and intermittent serum starvation can be related to changes in the transcription of genes responsible for cell metabolism and cancer’s stress pathways. Moreover, when we added cisplatin to the treatments, there was a strong enrichment of genes taking part in G2/M cell cycle arrest and apoptosis. |
| first_indexed | 2024-03-09T13:52:44Z |
| format | Article |
| id | doaj.art-1c7014e4d0514dfb85ad8be24dd5af12 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T13:52:44Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1c7014e4d0514dfb85ad8be24dd5af122023-11-30T20:46:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124191474310.3390/ijms241914743Transcriptome Analysis of Cisplatin, Cannabidiol, and Intermittent Serum Starvation Alone and in Various Combinations on Colorectal Cancer CellsViktoriia Cherkasova0Yaroslav Ilnytskyy1Olga Kovalchuk2Igor Kovalchuk3Department of Biological Sciences, University of Lethbridge, Lethbridge, AB T1K 3M4, CanadaDepartment of Biological Sciences, University of Lethbridge, Lethbridge, AB T1K 3M4, CanadaDepartment of Biological Sciences, University of Lethbridge, Lethbridge, AB T1K 3M4, CanadaDepartment of Biological Sciences, University of Lethbridge, Lethbridge, AB T1K 3M4, CanadaPlatinum-derived chemotherapy medications are often combined with other conventional therapies for treating different tumors, including colorectal cancer. However, the development of drug resistance and multiple adverse effects remain common in clinical settings. Thus, there is a necessity to find novel treatments and drug combinations that could effectively target colorectal cancer cells and lower the probability of disease relapse. To find potential synergistic interaction, we designed multiple different combinations between cisplatin, cannabidiol, and intermittent serum starvation on colorectal cancer cell lines. Based on the cell viability assay, we found that combinations between cannabidiol and intermittent serum starvation, cisplatin and intermittent serum starvation, as well as cisplatin, cannabidiol, and intermittent serum starvation can work in a synergistic fashion on different colorectal cancer cell lines. Furthermore, we analyzed differentially expressed genes and affected pathways in colorectal cancer cell lines to understand further the potential molecular mechanisms behind the treatments and their interactions. We found that synergistic interaction between cannabidiol and intermittent serum starvation can be related to changes in the transcription of genes responsible for cell metabolism and cancer’s stress pathways. Moreover, when we added cisplatin to the treatments, there was a strong enrichment of genes taking part in G2/M cell cycle arrest and apoptosis.https://www.mdpi.com/1422-0067/24/19/14743cisplatincannabidiolintermittent fastingserum starvationcolorectal cancer |
| spellingShingle | Viktoriia Cherkasova Yaroslav Ilnytskyy Olga Kovalchuk Igor Kovalchuk Transcriptome Analysis of Cisplatin, Cannabidiol, and Intermittent Serum Starvation Alone and in Various Combinations on Colorectal Cancer Cells International Journal of Molecular Sciences cisplatin cannabidiol intermittent fasting serum starvation colorectal cancer |
| title | Transcriptome Analysis of Cisplatin, Cannabidiol, and Intermittent Serum Starvation Alone and in Various Combinations on Colorectal Cancer Cells |
| title_full | Transcriptome Analysis of Cisplatin, Cannabidiol, and Intermittent Serum Starvation Alone and in Various Combinations on Colorectal Cancer Cells |
| title_fullStr | Transcriptome Analysis of Cisplatin, Cannabidiol, and Intermittent Serum Starvation Alone and in Various Combinations on Colorectal Cancer Cells |
| title_full_unstemmed | Transcriptome Analysis of Cisplatin, Cannabidiol, and Intermittent Serum Starvation Alone and in Various Combinations on Colorectal Cancer Cells |
| title_short | Transcriptome Analysis of Cisplatin, Cannabidiol, and Intermittent Serum Starvation Alone and in Various Combinations on Colorectal Cancer Cells |
| title_sort | transcriptome analysis of cisplatin cannabidiol and intermittent serum starvation alone and in various combinations on colorectal cancer cells |
| topic | cisplatin cannabidiol intermittent fasting serum starvation colorectal cancer |
| url | https://www.mdpi.com/1422-0067/24/19/14743 |
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