Symbiont modulates expression of specific gene categories in Angomonas deanei
Trypanosomatids are parasites that cause disease in humans, animals, and plants. Most are non-pathogenic and some harbor a symbiotic bacterium. Endosymbiosis is part of the evolutionary process of vital cell functions such as respiration and photosynthesis. Angomonas deanei is an example of a symbio...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016005019105&lng=en&tlng=en |
| _version_ | 1797715827373375488 |
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| author | Luciana Loureiro Penha Luísa Hoffmann Silvanna Sant’Anna de Souza Allan Cézar de Azevedo Martins Thayane Bottaro Francisco Prosdocimi Débora Souza Faffe Maria Cristina Machado Motta Turán Péter Ürményi Rosane Silva |
| author_facet | Luciana Loureiro Penha Luísa Hoffmann Silvanna Sant’Anna de Souza Allan Cézar de Azevedo Martins Thayane Bottaro Francisco Prosdocimi Débora Souza Faffe Maria Cristina Machado Motta Turán Péter Ürményi Rosane Silva |
| author_sort | Luciana Loureiro Penha |
| collection | DOAJ |
| description | Trypanosomatids are parasites that cause disease in humans, animals, and plants. Most are non-pathogenic and some harbor a symbiotic bacterium. Endosymbiosis is part of the evolutionary process of vital cell functions such as respiration and photosynthesis. Angomonas deanei is an example of a symbiont-containing trypanosomatid. In this paper, we sought to investigate how symbionts influence host cells by characterising and comparing the transcriptomes of the symbiont-containing A. deanei (wild type) and the symbiont-free aposymbiotic strains. The comparison revealed that the presence of the symbiont modulates several differentially expressed genes. Empirical analysis of differential gene expression showed that 216 of the 7625 modulated genes were significantly changed. Finally, gene set enrichment analysis revealed that the largest categories of genes that downregulated in the absence of the symbiont were those involved in oxidation-reduction process, ATP hydrolysis coupled proton transport and glycolysis. In contrast, among the upregulated gene categories were those involved in proteolysis, microtubule-based movement, and cellular metabolic process. Our results provide valuable information for dissecting the mechanism of endosymbiosis in A. deanei. |
| first_indexed | 2024-03-12T08:12:32Z |
| format | Article |
| id | doaj.art-1c717ffd478f48179fd539c2ab2f396e |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:12:32Z |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-1c717ffd478f48179fd539c2ab2f396e2023-09-02T19:06:31ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-8060010.1590/0074-02760160228S0074-02762016005019105Symbiont modulates expression of specific gene categories in Angomonas deaneiLuciana Loureiro PenhaLuísa HoffmannSilvanna Sant’Anna de SouzaAllan Cézar de Azevedo MartinsThayane BottaroFrancisco ProsdocimiDébora Souza FaffeMaria Cristina Machado MottaTurán Péter ÜrményiRosane SilvaTrypanosomatids are parasites that cause disease in humans, animals, and plants. Most are non-pathogenic and some harbor a symbiotic bacterium. Endosymbiosis is part of the evolutionary process of vital cell functions such as respiration and photosynthesis. Angomonas deanei is an example of a symbiont-containing trypanosomatid. In this paper, we sought to investigate how symbionts influence host cells by characterising and comparing the transcriptomes of the symbiont-containing A. deanei (wild type) and the symbiont-free aposymbiotic strains. The comparison revealed that the presence of the symbiont modulates several differentially expressed genes. Empirical analysis of differential gene expression showed that 216 of the 7625 modulated genes were significantly changed. Finally, gene set enrichment analysis revealed that the largest categories of genes that downregulated in the absence of the symbiont were those involved in oxidation-reduction process, ATP hydrolysis coupled proton transport and glycolysis. In contrast, among the upregulated gene categories were those involved in proteolysis, microtubule-based movement, and cellular metabolic process. Our results provide valuable information for dissecting the mechanism of endosymbiosis in A. deanei.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016005019105&lng=en&tlng=entrypanosomatid RNA-seqgene expressionaposymbiotic strainendosymbiosis |
| spellingShingle | Luciana Loureiro Penha Luísa Hoffmann Silvanna Sant’Anna de Souza Allan Cézar de Azevedo Martins Thayane Bottaro Francisco Prosdocimi Débora Souza Faffe Maria Cristina Machado Motta Turán Péter Ürményi Rosane Silva Symbiont modulates expression of specific gene categories in Angomonas deanei Memorias do Instituto Oswaldo Cruz trypanosomatid RNA-seq gene expression aposymbiotic strain endosymbiosis |
| title | Symbiont modulates expression of specific gene categories in Angomonas deanei |
| title_full | Symbiont modulates expression of specific gene categories in Angomonas deanei |
| title_fullStr | Symbiont modulates expression of specific gene categories in Angomonas deanei |
| title_full_unstemmed | Symbiont modulates expression of specific gene categories in Angomonas deanei |
| title_short | Symbiont modulates expression of specific gene categories in Angomonas deanei |
| title_sort | symbiont modulates expression of specific gene categories in angomonas deanei |
| topic | trypanosomatid RNA-seq gene expression aposymbiotic strain endosymbiosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016005019105&lng=en&tlng=en |
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