LOW-COST AND EASY ACCESS MATERIALS FOR A LABORATORY CLASS: A PROPOSAL OF LIQUID CHROMATOGRAPHY FOR FOOD COLORING SEPARATION AS AN EXPERIENCE OF MEANINGFUL LEARNING

The experimental activities for undergraduate chemistry students play an important role to develop skills and expertise in that science thus, the practice-experimental classes are so important to achieve this purpose. This work deals with a demonstrative experience for dye separation from food color...

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Main Authors: Bruna Costa Cerqueira, Gerônimo Lopes Lima, Artur José Santos Mascarenhas, Heloysa Martins Carvalho Andrade, Rodrigo De Paula
Format: Article
Language:English
Published: Sociedade Brasileira de Química 2023-06-01
Series:Química Nova
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422023000500482&tlng=en
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author Bruna Costa Cerqueira
Gerônimo Lopes Lima
Artur José Santos Mascarenhas
Heloysa Martins Carvalho Andrade
Rodrigo De Paula
author_facet Bruna Costa Cerqueira
Gerônimo Lopes Lima
Artur José Santos Mascarenhas
Heloysa Martins Carvalho Andrade
Rodrigo De Paula
author_sort Bruna Costa Cerqueira
collection DOAJ
description The experimental activities for undergraduate chemistry students play an important role to develop skills and expertise in that science thus, the practice-experimental classes are so important to achieve this purpose. This work deals with a demonstrative experience for dye separation from food coloring sample using liquid column chromatography. Beyond the experimental practice and the facility in visualizing the separation, this demonstration shows how a simple experiment involving alternative and easy access materials can promote a meaningful learning once it is possible to handle the acquired data, trace and analyze the results themselves, thus developing skills rather than the operational procedure. This proposal could be implemented in initial chemistry classes in Brazilian universities. Sea and river sands were used as stationary phases and aqueous ethanolic solutions were used as mobile phases. A food coloring (a mixture of 3 dyes) was chosen as sample to be studied. Chromatographic separation was followed by spectrophotometric measurements. A set of analyses were carried out to discuss the solubility of the food coloring, granulometric studies of the stationary phases used and electronic microscopy images of the sands studied here. As alternative materials were used in this work, a chromatographic column was also constructed with low-cost materials.
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spelling doaj.art-1c72b87c22f64f97a26b1a4c96fb9a8f2023-06-30T10:59:45ZengSociedade Brasileira de QuímicaQuímica Nova1678-70642023-06-0146548249010.21577/0100-4042.20230027LOW-COST AND EASY ACCESS MATERIALS FOR A LABORATORY CLASS: A PROPOSAL OF LIQUID CHROMATOGRAPHY FOR FOOD COLORING SEPARATION AS AN EXPERIENCE OF MEANINGFUL LEARNINGBruna Costa CerqueiraGerônimo Lopes LimaArtur José Santos MascarenhasHeloysa Martins Carvalho AndradeRodrigo De Paulahttps://orcid.org/0000-0001-8675-3385The experimental activities for undergraduate chemistry students play an important role to develop skills and expertise in that science thus, the practice-experimental classes are so important to achieve this purpose. This work deals with a demonstrative experience for dye separation from food coloring sample using liquid column chromatography. Beyond the experimental practice and the facility in visualizing the separation, this demonstration shows how a simple experiment involving alternative and easy access materials can promote a meaningful learning once it is possible to handle the acquired data, trace and analyze the results themselves, thus developing skills rather than the operational procedure. This proposal could be implemented in initial chemistry classes in Brazilian universities. Sea and river sands were used as stationary phases and aqueous ethanolic solutions were used as mobile phases. A food coloring (a mixture of 3 dyes) was chosen as sample to be studied. Chromatographic separation was followed by spectrophotometric measurements. A set of analyses were carried out to discuss the solubility of the food coloring, granulometric studies of the stationary phases used and electronic microscopy images of the sands studied here. As alternative materials were used in this work, a chromatographic column was also constructed with low-cost materials.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422023000500482&tlng=en
spellingShingle Bruna Costa Cerqueira
Gerônimo Lopes Lima
Artur José Santos Mascarenhas
Heloysa Martins Carvalho Andrade
Rodrigo De Paula
LOW-COST AND EASY ACCESS MATERIALS FOR A LABORATORY CLASS: A PROPOSAL OF LIQUID CHROMATOGRAPHY FOR FOOD COLORING SEPARATION AS AN EXPERIENCE OF MEANINGFUL LEARNING
Química Nova
title LOW-COST AND EASY ACCESS MATERIALS FOR A LABORATORY CLASS: A PROPOSAL OF LIQUID CHROMATOGRAPHY FOR FOOD COLORING SEPARATION AS AN EXPERIENCE OF MEANINGFUL LEARNING
title_full LOW-COST AND EASY ACCESS MATERIALS FOR A LABORATORY CLASS: A PROPOSAL OF LIQUID CHROMATOGRAPHY FOR FOOD COLORING SEPARATION AS AN EXPERIENCE OF MEANINGFUL LEARNING
title_fullStr LOW-COST AND EASY ACCESS MATERIALS FOR A LABORATORY CLASS: A PROPOSAL OF LIQUID CHROMATOGRAPHY FOR FOOD COLORING SEPARATION AS AN EXPERIENCE OF MEANINGFUL LEARNING
title_full_unstemmed LOW-COST AND EASY ACCESS MATERIALS FOR A LABORATORY CLASS: A PROPOSAL OF LIQUID CHROMATOGRAPHY FOR FOOD COLORING SEPARATION AS AN EXPERIENCE OF MEANINGFUL LEARNING
title_short LOW-COST AND EASY ACCESS MATERIALS FOR A LABORATORY CLASS: A PROPOSAL OF LIQUID CHROMATOGRAPHY FOR FOOD COLORING SEPARATION AS AN EXPERIENCE OF MEANINGFUL LEARNING
title_sort low cost and easy access materials for a laboratory class a proposal of liquid chromatography for food coloring separation as an experience of meaningful learning
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422023000500482&tlng=en
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