The H-NS Regulator Plays a Role in the Stress Induced by Carbapenemase Expression in <named-content content-type="genus-species">Acinetobacter baumannii</named-content>
ABSTRACT Disruption of the histone-like nucleoid structuring protein (H-NS) was shown to affect the ability of Gram-negative bacteria to regulate genes associated with virulence, persistence, stress response, quorum sensing, biosynthesis pathways, and cell adhesion. Here, we used the expression of m...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2020-08-01
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| Series: | mSphere |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/mSphere.00793-20 |
| _version_ | 1818932210784272384 |
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| author | Fanny Huang Noelle Fitchett Chelsea Razo-Gutierrez Casin Le Jasmine Martinez Grace Ra Carolina Lopez Lisandro J. Gonzalez Rodrigo Sieira Alejandro J. Vila Robert A. Bonomo Maria Soledad Ramirez |
| author_facet | Fanny Huang Noelle Fitchett Chelsea Razo-Gutierrez Casin Le Jasmine Martinez Grace Ra Carolina Lopez Lisandro J. Gonzalez Rodrigo Sieira Alejandro J. Vila Robert A. Bonomo Maria Soledad Ramirez |
| author_sort | Fanny Huang |
| collection | DOAJ |
| description | ABSTRACT Disruption of the histone-like nucleoid structuring protein (H-NS) was shown to affect the ability of Gram-negative bacteria to regulate genes associated with virulence, persistence, stress response, quorum sensing, biosynthesis pathways, and cell adhesion. Here, we used the expression of metallo-β-lactamases (MBLs), known to elicit envelope stress by the accumulation of toxic precursors in the periplasm, to interrogate the role of H-NS in Acinetobacter baumannii, together with other stressors. Using a multidrug-resistant A. baumannii strain, we observed that H-NS plays a role in alleviating the stress triggered by MBL toxic precursors and counteracts the effect of DNA-damaging agents, supporting its role in stress response. IMPORTANCE Carbapenem-resistant A. baumannii (CRAB) is recognized as one of the most threatening Gram-negative bacilli. H-NS is known to play a role in controlling the transcription of a variety of different genes, including those associated with the stress response, persistence, and virulence. In the present work, we uncovered a link between the role of H-NS in the A. baumannii stress response and its relationship with the envelope stress response and resistance to DNA-damaging agents. Overall, we posit a new role of H-NS, showing that H-NS serves to endure envelope stress and could also be a mechanism that alleviates the stress induced by MBL expression in A. baumannii. This could be an evolutionary advantage to further resist the action of carbapenems. |
| first_indexed | 2024-12-20T04:28:52Z |
| format | Article |
| id | doaj.art-1c7b862b9b994dc2a5e97af9c858d8fb |
| institution | Directory Open Access Journal |
| issn | 2379-5042 |
| language | English |
| last_indexed | 2024-12-20T04:28:52Z |
| publishDate | 2020-08-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj.art-1c7b862b9b994dc2a5e97af9c858d8fb2022-12-21T19:53:27ZengAmerican Society for MicrobiologymSphere2379-50422020-08-015410.1128/mSphere.00793-20The H-NS Regulator Plays a Role in the Stress Induced by Carbapenemase Expression in <named-content content-type="genus-species">Acinetobacter baumannii</named-content>Fanny Huang0Noelle Fitchett1Chelsea Razo-Gutierrez2Casin Le3Jasmine Martinez4Grace Ra5Carolina Lopez6Lisandro J. Gonzalez7Rodrigo Sieira8Alejandro J. Vila9Robert A. Bonomo10Maria Soledad Ramirez11Center for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University, Fullerton, Fullerton, California, USACenter for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University, Fullerton, Fullerton, California, USACenter for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University, Fullerton, Fullerton, California, USACenter for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University, Fullerton, Fullerton, California, USACenter for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University, Fullerton, Fullerton, California, USACenter for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University, Fullerton, Fullerton, California, USAInstituto de Biología Molecular y Celular de Rosario (IBR, CONICET-UNR), Rosario, ArgentinaInstituto de Biología Molecular y Celular de Rosario (IBR, CONICET-UNR), Rosario, ArgentinaFundación Instituto Leloir—IIBBA CONICET, Buenos Aires, ArgentinaInstituto de Biología Molecular y Celular de Rosario (IBR, CONICET-UNR), Rosario, ArgentinaResearch Service and GRECC, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USACenter for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University, Fullerton, Fullerton, California, USAABSTRACT Disruption of the histone-like nucleoid structuring protein (H-NS) was shown to affect the ability of Gram-negative bacteria to regulate genes associated with virulence, persistence, stress response, quorum sensing, biosynthesis pathways, and cell adhesion. Here, we used the expression of metallo-β-lactamases (MBLs), known to elicit envelope stress by the accumulation of toxic precursors in the periplasm, to interrogate the role of H-NS in Acinetobacter baumannii, together with other stressors. Using a multidrug-resistant A. baumannii strain, we observed that H-NS plays a role in alleviating the stress triggered by MBL toxic precursors and counteracts the effect of DNA-damaging agents, supporting its role in stress response. IMPORTANCE Carbapenem-resistant A. baumannii (CRAB) is recognized as one of the most threatening Gram-negative bacilli. H-NS is known to play a role in controlling the transcription of a variety of different genes, including those associated with the stress response, persistence, and virulence. In the present work, we uncovered a link between the role of H-NS in the A. baumannii stress response and its relationship with the envelope stress response and resistance to DNA-damaging agents. Overall, we posit a new role of H-NS, showing that H-NS serves to endure envelope stress and could also be a mechanism that alleviates the stress induced by MBL expression in A. baumannii. This could be an evolutionary advantage to further resist the action of carbapenems.https://journals.asm.org/doi/10.1128/mSphere.00793-20Acinetobacter baumanniiH-NSstresscarbapenemasesAcinetobactercarbapenems |
| spellingShingle | Fanny Huang Noelle Fitchett Chelsea Razo-Gutierrez Casin Le Jasmine Martinez Grace Ra Carolina Lopez Lisandro J. Gonzalez Rodrigo Sieira Alejandro J. Vila Robert A. Bonomo Maria Soledad Ramirez The H-NS Regulator Plays a Role in the Stress Induced by Carbapenemase Expression in <named-content content-type="genus-species">Acinetobacter baumannii</named-content> mSphere Acinetobacter baumannii H-NS stress carbapenemases Acinetobacter carbapenems |
| title | The H-NS Regulator Plays a Role in the Stress Induced by Carbapenemase Expression in <named-content content-type="genus-species">Acinetobacter baumannii</named-content> |
| title_full | The H-NS Regulator Plays a Role in the Stress Induced by Carbapenemase Expression in <named-content content-type="genus-species">Acinetobacter baumannii</named-content> |
| title_fullStr | The H-NS Regulator Plays a Role in the Stress Induced by Carbapenemase Expression in <named-content content-type="genus-species">Acinetobacter baumannii</named-content> |
| title_full_unstemmed | The H-NS Regulator Plays a Role in the Stress Induced by Carbapenemase Expression in <named-content content-type="genus-species">Acinetobacter baumannii</named-content> |
| title_short | The H-NS Regulator Plays a Role in the Stress Induced by Carbapenemase Expression in <named-content content-type="genus-species">Acinetobacter baumannii</named-content> |
| title_sort | h ns regulator plays a role in the stress induced by carbapenemase expression in named content content type genus species acinetobacter baumannii named content |
| topic | Acinetobacter baumannii H-NS stress carbapenemases Acinetobacter carbapenems |
| url | https://journals.asm.org/doi/10.1128/mSphere.00793-20 |
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