Day-Night Variations in the Concentration of Neurotransmitters in the Rat Lumbar Spinal Cord
The purpose of this study was to analyze the light-dark variations in the concentrations of several neurotransmitters in the lumbar spinal cord of rats. Six groups of male Wistar rats were exposed to a 12 h light-12 h dark cycle for 70 days. At different time points of the experimental day (8, 12, 1...
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| Format: | Article |
| Language: | English |
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Ubiquity Press
2021-07-01
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| Series: | Journal of Circadian Rhythms |
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| Online Access: | https://www.jcircadianrhythms.com/articles/215 |
| _version_ | 1818726179922771968 |
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| author | Beatriz Shantal Jiménez-Zárate Celia Piña-Leyva Marina Rodríguez-Sánchez Benjamín Florán-Garduño Luis Antonio Jiménez-Zamudio Ismael Jiménez-Estrada |
| author_facet | Beatriz Shantal Jiménez-Zárate Celia Piña-Leyva Marina Rodríguez-Sánchez Benjamín Florán-Garduño Luis Antonio Jiménez-Zamudio Ismael Jiménez-Estrada |
| author_sort | Beatriz Shantal Jiménez-Zárate |
| collection | DOAJ |
| description | The purpose of this study was to analyze the light-dark variations in the concentrations of several neurotransmitters in the lumbar spinal cord of rats. Six groups of male Wistar rats were exposed to a 12 h light-12 h dark cycle for 70 days. At different time points of the experimental day (8, 12, 16, 20, 24 and 4 h), one of the groups of rats was randomly selected to be sacrificed, and the spinal cords were removed. The gamma-aminobutyric acid (GABA), glutamate (GLU), dopamine, serotonin, epinephrine (E), and norepinephrine (NE) levels in each extracted spinal cord were measured with high-pressure liquid chromatography (HPLC)-EQ and HPLC-fluorescence systems. Our results indicate that the spinal concentrations of GABA and GLU showed sinusoidal variation in a 24 h cycle, with the highest peak in the dark period (~20 h). Dopamine and serotonin also fluctuated in concentration but peaked in the light period (between 8 and 12 h), while E and NE concentrations showed no significant fluctuations. The possible relationship between neurotransmitter spinal concentration and sensitivity to pain and locomotor activity is discussed. It was concluded that most of the neurotransmitter levels in the lumbar spinal cord showed circadian fluctuations coupled to a light-dark cycle. |
| first_indexed | 2024-12-17T21:54:06Z |
| format | Article |
| id | doaj.art-1c7bb111c8a942dd96d30a0d6fbac7ea |
| institution | Directory Open Access Journal |
| issn | 1740-3391 |
| language | English |
| last_indexed | 2024-12-17T21:54:06Z |
| publishDate | 2021-07-01 |
| publisher | Ubiquity Press |
| record_format | Article |
| series | Journal of Circadian Rhythms |
| spelling | doaj.art-1c7bb111c8a942dd96d30a0d6fbac7ea2022-12-21T21:31:10ZengUbiquity PressJournal of Circadian Rhythms1740-33912021-07-0119110.5334/jcr.215177Day-Night Variations in the Concentration of Neurotransmitters in the Rat Lumbar Spinal CordBeatriz Shantal Jiménez-Zárate0Celia Piña-Leyva1Marina Rodríguez-Sánchez2Benjamín Florán-Garduño3Luis Antonio Jiménez-Zamudio4Ismael Jiménez-Estrada5Department of Physiology, Biophysics and Neurosciences, CINVESTAV, IPN; Department of Physiology, ENCB, IPN, México CityDepartment of Physiology, Biophysics and Neurosciences, CINVESTAV, IPNDepartment of Pharmacology, CINVESTAV, IPNDepartment of Physiology, Biophysics and Neurosciences, CINVESTAV, IPNDepartment of Immunology, ENCB, IPN, México CityDepartment of Physiology, Biophysics and Neurosciences, CINVESTAV, IPNThe purpose of this study was to analyze the light-dark variations in the concentrations of several neurotransmitters in the lumbar spinal cord of rats. Six groups of male Wistar rats were exposed to a 12 h light-12 h dark cycle for 70 days. At different time points of the experimental day (8, 12, 16, 20, 24 and 4 h), one of the groups of rats was randomly selected to be sacrificed, and the spinal cords were removed. The gamma-aminobutyric acid (GABA), glutamate (GLU), dopamine, serotonin, epinephrine (E), and norepinephrine (NE) levels in each extracted spinal cord were measured with high-pressure liquid chromatography (HPLC)-EQ and HPLC-fluorescence systems. Our results indicate that the spinal concentrations of GABA and GLU showed sinusoidal variation in a 24 h cycle, with the highest peak in the dark period (~20 h). Dopamine and serotonin also fluctuated in concentration but peaked in the light period (between 8 and 12 h), while E and NE concentrations showed no significant fluctuations. The possible relationship between neurotransmitter spinal concentration and sensitivity to pain and locomotor activity is discussed. It was concluded that most of the neurotransmitter levels in the lumbar spinal cord showed circadian fluctuations coupled to a light-dark cycle.https://www.jcircadianrhythms.com/articles/215neurotransmitter concentrationspinal cordcircadian rhythmslumbar |
| spellingShingle | Beatriz Shantal Jiménez-Zárate Celia Piña-Leyva Marina Rodríguez-Sánchez Benjamín Florán-Garduño Luis Antonio Jiménez-Zamudio Ismael Jiménez-Estrada Day-Night Variations in the Concentration of Neurotransmitters in the Rat Lumbar Spinal Cord Journal of Circadian Rhythms neurotransmitter concentration spinal cord circadian rhythms lumbar |
| title | Day-Night Variations in the Concentration of Neurotransmitters in the Rat Lumbar Spinal Cord |
| title_full | Day-Night Variations in the Concentration of Neurotransmitters in the Rat Lumbar Spinal Cord |
| title_fullStr | Day-Night Variations in the Concentration of Neurotransmitters in the Rat Lumbar Spinal Cord |
| title_full_unstemmed | Day-Night Variations in the Concentration of Neurotransmitters in the Rat Lumbar Spinal Cord |
| title_short | Day-Night Variations in the Concentration of Neurotransmitters in the Rat Lumbar Spinal Cord |
| title_sort | day night variations in the concentration of neurotransmitters in the rat lumbar spinal cord |
| topic | neurotransmitter concentration spinal cord circadian rhythms lumbar |
| url | https://www.jcircadianrhythms.com/articles/215 |
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