hsa_circ_0111707 Is Associated With Risk of Stress-Related Type 2 Diabetes via Sponging miR-144-3p
IntroductionChronic stress plays an important role in the development of type 2 diabetes (T2D). Circular RNAs (circRNAs) play significant roles in regulating the pathogenesis of diseases by regulating gene expression. The aim of the present study was to identify the association between hsa_circ_0111...
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2021.790591/full |
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| author | Yu-Xiang Yan Yu-Xiang Yan Huan-Bo Xiao Ya-Ke Lu Ya-Ke Lu Yue Sun Shuo Wang Jing Dong Li-Juan Wu Li-Juan Wu |
| author_facet | Yu-Xiang Yan Yu-Xiang Yan Huan-Bo Xiao Ya-Ke Lu Ya-Ke Lu Yue Sun Shuo Wang Jing Dong Li-Juan Wu Li-Juan Wu |
| author_sort | Yu-Xiang Yan |
| collection | DOAJ |
| description | IntroductionChronic stress plays an important role in the development of type 2 diabetes (T2D). Circular RNAs (circRNAs) play significant roles in regulating the pathogenesis of diseases by regulating gene expression. The aim of the present study was to identify the association between hsa_circ_0111707 and stress-related T2D.MethodsThe present study was performed based on a three-part design. The association between hsa_circ_0111707 in peripheral blood mononuclear cells (PBMCs) and T2D and stress-related variables were assessed in a cross-sectional study. The causal relationship of hsa_circ_0111707 on T2D was further investigated in a nested case-control study. miR-144-3p as the miRNA target of hsa_circ_0111707 was verified by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay.ResultsThe relative expression of hsa_circ_0111707 was significantly lower in the T2D and impaired fasting glucose (IFG) cases in comparison with controls. The hsa_circ_0111707 expression was significantly negatively correlated with miR-144-3p expression and plasma cortisol concentration and positively correlated with NR3C1 expression. In addition, hsa_circ_0111707 expression was negatively correlated with scores of “demands at work” and “insecurity at work” of Copenhagen Psychosocial Questionnaire (COPSOQ). Decreased hsa_circ_0111707 expression was associated with increased risk of T2D development. Functional analysis demonstrated that hsa_circ_0111707 functions as a sponge for miR-144-3p.Conclusionhsa_circ_0111707 is associated with risk of T2D development via sponging miR-144-3p. hsa_circ_0111707 in PBMCs can be considered a potential biomarker of stress-related T2D. |
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| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-20T19:16:24Z |
| publishDate | 2022-01-01 |
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| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-1c7cdf6c00d74f57bc0b02ab23e073802022-12-21T19:29:05ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-01-011210.3389/fendo.2021.790591790591hsa_circ_0111707 Is Associated With Risk of Stress-Related Type 2 Diabetes via Sponging miR-144-3pYu-Xiang Yan0Yu-Xiang Yan1Huan-Bo Xiao2Ya-Ke Lu3Ya-Ke Lu4Yue Sun5Shuo Wang6Jing Dong7Li-Juan Wu8Li-Juan Wu9Department of Epidemiology and Biostatistics, School of Public Health, Capital Medical University, Beijing, ChinaMunicipal Key Laboratory of Clinical Epidemiology, Beijing, ChinaDepartment of Preventive Medicine, Yanjing Medical College, Capital Medical University, Beijing, ChinaDepartment of Epidemiology and Biostatistics, School of Public Health, Capital Medical University, Beijing, ChinaMunicipal Key Laboratory of Clinical Epidemiology, Beijing, ChinaDepartment of Epidemiology and Biostatistics, School of Public Health, Capital Medical University, Beijing, ChinaDepartment of Epidemiology and Biostatistics, School of Public Health, Capital Medical University, Beijing, ChinaHealth Management Center, Xuanwu Hospital, Capital Medical University, Beijing, ChinaDepartment of Epidemiology and Biostatistics, School of Public Health, Capital Medical University, Beijing, ChinaMunicipal Key Laboratory of Clinical Epidemiology, Beijing, ChinaIntroductionChronic stress plays an important role in the development of type 2 diabetes (T2D). Circular RNAs (circRNAs) play significant roles in regulating the pathogenesis of diseases by regulating gene expression. The aim of the present study was to identify the association between hsa_circ_0111707 and stress-related T2D.MethodsThe present study was performed based on a three-part design. The association between hsa_circ_0111707 in peripheral blood mononuclear cells (PBMCs) and T2D and stress-related variables were assessed in a cross-sectional study. The causal relationship of hsa_circ_0111707 on T2D was further investigated in a nested case-control study. miR-144-3p as the miRNA target of hsa_circ_0111707 was verified by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay.ResultsThe relative expression of hsa_circ_0111707 was significantly lower in the T2D and impaired fasting glucose (IFG) cases in comparison with controls. The hsa_circ_0111707 expression was significantly negatively correlated with miR-144-3p expression and plasma cortisol concentration and positively correlated with NR3C1 expression. In addition, hsa_circ_0111707 expression was negatively correlated with scores of “demands at work” and “insecurity at work” of Copenhagen Psychosocial Questionnaire (COPSOQ). Decreased hsa_circ_0111707 expression was associated with increased risk of T2D development. Functional analysis demonstrated that hsa_circ_0111707 functions as a sponge for miR-144-3p.Conclusionhsa_circ_0111707 is associated with risk of T2D development via sponging miR-144-3p. hsa_circ_0111707 in PBMCs can be considered a potential biomarker of stress-related T2D.https://www.frontiersin.org/articles/10.3389/fendo.2021.790591/fullcircRNAtype 2 diabetesbiomarkerneuroendocrinestress response |
| spellingShingle | Yu-Xiang Yan Yu-Xiang Yan Huan-Bo Xiao Ya-Ke Lu Ya-Ke Lu Yue Sun Shuo Wang Jing Dong Li-Juan Wu Li-Juan Wu hsa_circ_0111707 Is Associated With Risk of Stress-Related Type 2 Diabetes via Sponging miR-144-3p Frontiers in Endocrinology circRNA type 2 diabetes biomarker neuroendocrine stress response |
| title | hsa_circ_0111707 Is Associated With Risk of Stress-Related Type 2 Diabetes via Sponging miR-144-3p |
| title_full | hsa_circ_0111707 Is Associated With Risk of Stress-Related Type 2 Diabetes via Sponging miR-144-3p |
| title_fullStr | hsa_circ_0111707 Is Associated With Risk of Stress-Related Type 2 Diabetes via Sponging miR-144-3p |
| title_full_unstemmed | hsa_circ_0111707 Is Associated With Risk of Stress-Related Type 2 Diabetes via Sponging miR-144-3p |
| title_short | hsa_circ_0111707 Is Associated With Risk of Stress-Related Type 2 Diabetes via Sponging miR-144-3p |
| title_sort | hsa circ 0111707 is associated with risk of stress related type 2 diabetes via sponging mir 144 3p |
| topic | circRNA type 2 diabetes biomarker neuroendocrine stress response |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2021.790591/full |
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