Biosensor-Coupled In Vivo Mutagenesis and Omics Analysis Reveals Reduced Lysine and Arginine Synthesis To Improve Malonyl-Coenzyme A Flux in Saccharomyces cerevisiae

Biosensor-Coupled In Vivo Mutagenesis and Omics Analysis Reveals Reduced Lysine and Arginine Synthesis To Improve Malonyl-Coenzyme A Flux in Saccharomyces cerevisiae

ABSTRACT Malonyl-coenzyme A (malonyl-CoA) is an important precursor for producing various chemicals, but its low availability limits the synthesis of downstream products in Saccharomyces cerevisiae. Owing to the complexity of metabolism, evolutionary engineering is required for developing strains wi...

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Bibliographic Details
Main Authors: Chenxi Qiu, Mingtao Huang, Yishan Hou, Huilin Tao, Jianzhi Zhao, Yu Shen, Xiaoming Bao, Qingsheng Qi, Jin Hou
Format: Article
Language:English
Published: American Society for Microbiology 2022-04-01
Series:mSystems
Subjects:
malonyl-CoA biosensor
growth-based screening
in vivo mutagenesis
omics analysis
Saccharomyces cerevisiae
Online Access:https://journals.asm.org/doi/10.1128/msystems.01366-21

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