HPV DNA/RNA detection in various oral and oropharyngeal biomaterials identifies active HPV infections also in non-neoplastic tonsils
Previous studies describe a correlation between HPV-positivity and non-smoking in TSCC; p16INK4A-expression as surrogate-marker for HPV-DNA/RNA-positivity is discussed controversially. In the present study, these parameters are assessed prospectively. HPV-status of sputum and tonsillar-swabs was ana...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-02-01
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| Series: | Translational Oncology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1936523320304940 |
| _version_ | 1819296308964360192 |
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| author | Elgar Susanne Quabius Silke Tribius Alessa Heinrichs Dirk Haaser André Kühnel Martin Laudien Florian Hoppe Robert Mlynski Petra Ambrosch Markus Hoffmann |
| author_facet | Elgar Susanne Quabius Silke Tribius Alessa Heinrichs Dirk Haaser André Kühnel Martin Laudien Florian Hoppe Robert Mlynski Petra Ambrosch Markus Hoffmann |
| author_sort | Elgar Susanne Quabius |
| collection | DOAJ |
| description | Previous studies describe a correlation between HPV-positivity and non-smoking in TSCC; p16INK4A-expression as surrogate-marker for HPV-DNA/RNA-positivity is discussed controversially. In the present study, these parameters are assessed prospectively. HPV-status of sputum and tonsillar-swabs was analyzed to determine their validity as surrogate-marker for tissue-HPV-status.TSCC- (n = 52) and non-neoplastic tonsillar tissue (n = 163) were analyzed. HPV-DNA- and HPV-RNA-status of total sputum, cellular fraction and supernatants, tonsillar-swabs and -tissue was determined by (RT)-PCR. Immunohistochemistry determined p16INK4A-expression.23/163 (14.2%) non-neoplastic tonsils were HPV-DNA-positive; five patients (3 HPV16, 2 HPV11) had active HPV-infections (HPV-RNA-positive), in all biomaterials. 140/163 (85.9%) patients were either HPV-DNA-positive or HPV-DNA-negative in all samples. 21/52 (40.4%) TSCC-tonsils were HPV-DNA-positive; 17 patients were HPV-RNA-positive (14 HPV16; 4 HPV18). 40/52 (76.9%) TSCC-patients were congruent in all biomaterials. p16INK4A-expression alone would have misclassified the HPV-status of 14/52 (26.2%) TSCC-patients.This prospective study confirms the discrepancy between HPV-status and p16INK4A-expression and the significant correlation between non-smoking and HPV-DNA-positivity. HPV-sputum- and/or swab-results do not consistently match tissue-results, possibly having (detrimental) consequences if those were used to assess tissue-HPV-status. In the 5 patients with active HPV infection in the non-neoplasitic tonsils, tonsillectomy likely prevented subsequent development of TSCC. |
| first_indexed | 2024-12-24T04:56:03Z |
| format | Article |
| id | doaj.art-1c8252b29c84409b829606f77e29bd8b |
| institution | Directory Open Access Journal |
| issn | 1936-5233 |
| language | English |
| last_indexed | 2024-12-24T04:56:03Z |
| publishDate | 2021-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj.art-1c8252b29c84409b829606f77e29bd8b2022-12-21T17:14:24ZengElsevierTranslational Oncology1936-52332021-02-01142101002HPV DNA/RNA detection in various oral and oropharyngeal biomaterials identifies active HPV infections also in non-neoplastic tonsilsElgar Susanne Quabius0Silke Tribius1Alessa Heinrichs2Dirk Haaser3André Kühnel4Martin Laudien5Florian Hoppe6Robert Mlynski7Petra Ambrosch8Markus Hoffmann9Department of Otorhinolaryngology, Head and Neck Surgery, Christian-Albrechts-University Kiel, Arnold-Heller-Str. 3, Building 27, D24105 Kiel, GermanyHermann-Holthusen-Institute for Radiation Oncology, Asklepios Hospital St. Georg, Hamburg, GermanyDepartment of Otorhinolaryngology, Head and Neck Surgery, University of Rostock, GermanyDepartment of Otorhinolaryngology, Head and Neck Surgery, Christian-Albrechts-University Kiel, Arnold-Heller-Str. 3, Building 27, D24105 Kiel, GermanyDepartment of Otorhinolaryngology, Head and Neck Surgery, Asklepios Hospital Harburg, Hamburg, GermanyDepartment of Otorhinolaryngology, Head and Neck Surgery, Christian-Albrechts-University Kiel, Arnold-Heller-Str. 3, Building 27, D24105 Kiel, GermanyDepartment of Otorhinolaryngology-Head and Neck Surgery, Klinikum Oldenburg, Hoppe, GermanyDepartment of Otorhinolaryngology, Head and Neck Surgery, University of Rostock, GermanyDepartment of Otorhinolaryngology, Head and Neck Surgery, Christian-Albrechts-University Kiel, Arnold-Heller-Str. 3, Building 27, D24105 Kiel, GermanyDepartment of Otorhinolaryngology, Head and Neck Surgery, Christian-Albrechts-University Kiel, Arnold-Heller-Str. 3, Building 27, D24105 Kiel, Germany; Corresponding author.Previous studies describe a correlation between HPV-positivity and non-smoking in TSCC; p16INK4A-expression as surrogate-marker for HPV-DNA/RNA-positivity is discussed controversially. In the present study, these parameters are assessed prospectively. HPV-status of sputum and tonsillar-swabs was analyzed to determine their validity as surrogate-marker for tissue-HPV-status.TSCC- (n = 52) and non-neoplastic tonsillar tissue (n = 163) were analyzed. HPV-DNA- and HPV-RNA-status of total sputum, cellular fraction and supernatants, tonsillar-swabs and -tissue was determined by (RT)-PCR. Immunohistochemistry determined p16INK4A-expression.23/163 (14.2%) non-neoplastic tonsils were HPV-DNA-positive; five patients (3 HPV16, 2 HPV11) had active HPV-infections (HPV-RNA-positive), in all biomaterials. 140/163 (85.9%) patients were either HPV-DNA-positive or HPV-DNA-negative in all samples. 21/52 (40.4%) TSCC-tonsils were HPV-DNA-positive; 17 patients were HPV-RNA-positive (14 HPV16; 4 HPV18). 40/52 (76.9%) TSCC-patients were congruent in all biomaterials. p16INK4A-expression alone would have misclassified the HPV-status of 14/52 (26.2%) TSCC-patients.This prospective study confirms the discrepancy between HPV-status and p16INK4A-expression and the significant correlation between non-smoking and HPV-DNA-positivity. HPV-sputum- and/or swab-results do not consistently match tissue-results, possibly having (detrimental) consequences if those were used to assess tissue-HPV-status. In the 5 patients with active HPV infection in the non-neoplasitic tonsils, tonsillectomy likely prevented subsequent development of TSCC.http://www.sciencedirect.com/science/article/pii/S1936523320304940TissueGargleSwabs sputumHPVTSCCTonsils |
| spellingShingle | Elgar Susanne Quabius Silke Tribius Alessa Heinrichs Dirk Haaser André Kühnel Martin Laudien Florian Hoppe Robert Mlynski Petra Ambrosch Markus Hoffmann HPV DNA/RNA detection in various oral and oropharyngeal biomaterials identifies active HPV infections also in non-neoplastic tonsils Translational Oncology Tissue Gargle Swabs sputum HPV TSCC Tonsils |
| title | HPV DNA/RNA detection in various oral and oropharyngeal biomaterials identifies active HPV infections also in non-neoplastic tonsils |
| title_full | HPV DNA/RNA detection in various oral and oropharyngeal biomaterials identifies active HPV infections also in non-neoplastic tonsils |
| title_fullStr | HPV DNA/RNA detection in various oral and oropharyngeal biomaterials identifies active HPV infections also in non-neoplastic tonsils |
| title_full_unstemmed | HPV DNA/RNA detection in various oral and oropharyngeal biomaterials identifies active HPV infections also in non-neoplastic tonsils |
| title_short | HPV DNA/RNA detection in various oral and oropharyngeal biomaterials identifies active HPV infections also in non-neoplastic tonsils |
| title_sort | hpv dna rna detection in various oral and oropharyngeal biomaterials identifies active hpv infections also in non neoplastic tonsils |
| topic | Tissue Gargle Swabs sputum HPV TSCC Tonsils |
| url | http://www.sciencedirect.com/science/article/pii/S1936523320304940 |
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