A Hands-on Activity for V(D)J Recombination in an Undergraduate Immunology Class
ABSTRACT This learning activity teaches the difficult concept of V(D)J recombination as it occurs in B cells. Following the traditional lecture, this hands-on activity uses pipe cleaners of various colors representing variable, joining, and diversity gene segments and recombination signal sequences....
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2023-04-01
|
| Series: | Journal of Microbiology & Biology Education |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/jmbe.00145-22 |
| _version_ | 1827963878683705344 |
|---|---|
| author | Kelly N. Hallstrom Gwendowlyn S. Knapp |
| author_facet | Kelly N. Hallstrom Gwendowlyn S. Knapp |
| author_sort | Kelly N. Hallstrom |
| collection | DOAJ |
| description | ABSTRACT This learning activity teaches the difficult concept of V(D)J recombination as it occurs in B cells. Following the traditional lecture, this hands-on activity uses pipe cleaners of various colors representing variable, joining, and diversity gene segments and recombination signal sequences. Students are provided with instructions for using the pipe cleaners to assemble specific light and heavy immunoglobulin chains. Students each assemble their own light and heavy chains and compare the products made by classmates. This activity uses materials that are easy and affordable to acquire and provides a tactile approach to reinforcing concepts that students often struggle to visualize and master from lecture and textbook material alone. |
| first_indexed | 2024-04-09T17:06:04Z |
| format | Article |
| id | doaj.art-1c82d442da8e42e3b3e414fdb509c314 |
| institution | Directory Open Access Journal |
| issn | 1935-7877 1935-7885 |
| language | English |
| last_indexed | 2024-04-09T17:06:04Z |
| publishDate | 2023-04-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Journal of Microbiology & Biology Education |
| spelling | doaj.art-1c82d442da8e42e3b3e414fdb509c3142023-04-20T13:01:01ZengAmerican Society for MicrobiologyJournal of Microbiology & Biology Education1935-78771935-78852023-04-0124110.1128/jmbe.00145-22A Hands-on Activity for V(D)J Recombination in an Undergraduate Immunology ClassKelly N. Hallstrom0Gwendowlyn S. Knapp1Department of Physical and Biological Sciences, College of St. Rose, Albany, New York, USADepartment of Biology, Illinois College, Jacksonville, Illinois, USAABSTRACT This learning activity teaches the difficult concept of V(D)J recombination as it occurs in B cells. Following the traditional lecture, this hands-on activity uses pipe cleaners of various colors representing variable, joining, and diversity gene segments and recombination signal sequences. Students are provided with instructions for using the pipe cleaners to assemble specific light and heavy immunoglobulin chains. Students each assemble their own light and heavy chains and compare the products made by classmates. This activity uses materials that are easy and affordable to acquire and provides a tactile approach to reinforcing concepts that students often struggle to visualize and master from lecture and textbook material alone.https://journals.asm.org/doi/10.1128/jmbe.00145-22immunology laboratoryremote learning activitiestactile learningundergraduate immunologyV(D)J recombination |
| spellingShingle | Kelly N. Hallstrom Gwendowlyn S. Knapp A Hands-on Activity for V(D)J Recombination in an Undergraduate Immunology Class Journal of Microbiology & Biology Education immunology laboratory remote learning activities tactile learning undergraduate immunology V(D)J recombination |
| title | A Hands-on Activity for V(D)J Recombination in an Undergraduate Immunology Class |
| title_full | A Hands-on Activity for V(D)J Recombination in an Undergraduate Immunology Class |
| title_fullStr | A Hands-on Activity for V(D)J Recombination in an Undergraduate Immunology Class |
| title_full_unstemmed | A Hands-on Activity for V(D)J Recombination in an Undergraduate Immunology Class |
| title_short | A Hands-on Activity for V(D)J Recombination in an Undergraduate Immunology Class |
| title_sort | hands on activity for v d j recombination in an undergraduate immunology class |
| topic | immunology laboratory remote learning activities tactile learning undergraduate immunology V(D)J recombination |
| url | https://journals.asm.org/doi/10.1128/jmbe.00145-22 |
| work_keys_str_mv | AT kellynhallstrom ahandsonactivityforvdjrecombinationinanundergraduateimmunologyclass AT gwendowlynsknapp ahandsonactivityforvdjrecombinationinanundergraduateimmunologyclass AT kellynhallstrom handsonactivityforvdjrecombinationinanundergraduateimmunologyclass AT gwendowlynsknapp handsonactivityforvdjrecombinationinanundergraduateimmunologyclass |