Genetically Modified Soybean Detection Using a Biosensor Electrode with a Self-Assembled Monolayer of Gold Nanoparticles
In this study, we proposed a genosensor that can qualitatively and quantitatively detect genetically modified soybeans using a simple electrode with evenly distributed single layer gold nanoparticles. The DNA sensing electrode is made by sputtering a gold film on the substrate, and then sequentially...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/4/207 |
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| author | Cheng-Chi Chou Ying-Ting Lin Iren Kuznetsova Gou-Jen Wang |
| author_facet | Cheng-Chi Chou Ying-Ting Lin Iren Kuznetsova Gou-Jen Wang |
| author_sort | Cheng-Chi Chou |
| collection | DOAJ |
| description | In this study, we proposed a genosensor that can qualitatively and quantitatively detect genetically modified soybeans using a simple electrode with evenly distributed single layer gold nanoparticles. The DNA sensing electrode is made by sputtering a gold film on the substrate, and then sequentially depositing 1,6-hexanedithiol and gold nanoparticles with sulfur groups on the substrate. Then, the complementary to the CaMV 35S promoter (P35S) was used as the capture probe. The target DNA directly extracted from the genetically modified soybeans rather than the synthesized DNA segments was used to construct the detection standard curve. The experimental results showed that our genosensor could directly detect genetically modified genes extracted from soybeans. We obtained two percentage calibration curves. The calibration curve corresponding to the lower percentage range (1–6%) exhibits a sensitivity of 2.36 Ω/% with R<sup>2</sup> = 0.9983, while the calibration curve corresponding to the higher percentage range (6–40%) possesses a sensitivity of 0.1 Ω/% with R<sup>2</sup> = 0.9928. The limit of detection would be 1%. The recovery rates for the 4% and 5.7% GMS DNA were measured to be 104.1% and 102.49% with RSD at 6.24% and 2.54%. The gold nanoparticle sensing electrode developed in this research is suitable for qualitative and quantitative detection of genetically modified soybeans and can be further applied to the detection of other genetically modified crops in the future. |
| first_indexed | 2024-03-09T11:05:51Z |
| format | Article |
| id | doaj.art-1c84df571b9248dfbeb72dc21cec3f63 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T11:05:51Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-1c84df571b9248dfbeb72dc21cec3f632023-12-01T00:57:52ZengMDPI AGBiosensors2079-63742022-03-0112420710.3390/bios12040207Genetically Modified Soybean Detection Using a Biosensor Electrode with a Self-Assembled Monolayer of Gold NanoparticlesCheng-Chi Chou0Ying-Ting Lin1Iren Kuznetsova2Gou-Jen Wang3Department of Mechanical Engineering, National Chung-Hsing University, Taichung 40227, TaiwanProgram in Tissue Engineering and Regenerative Medicine, National Chung-Hsing University, Taichung 40227, TaiwanKotelnikov Institute of Radio Engineering and Electronics, Russian Academy of Science, 125009 Moscow, RussiaDepartment of Mechanical Engineering, National Chung-Hsing University, Taichung 40227, TaiwanIn this study, we proposed a genosensor that can qualitatively and quantitatively detect genetically modified soybeans using a simple electrode with evenly distributed single layer gold nanoparticles. The DNA sensing electrode is made by sputtering a gold film on the substrate, and then sequentially depositing 1,6-hexanedithiol and gold nanoparticles with sulfur groups on the substrate. Then, the complementary to the CaMV 35S promoter (P35S) was used as the capture probe. The target DNA directly extracted from the genetically modified soybeans rather than the synthesized DNA segments was used to construct the detection standard curve. The experimental results showed that our genosensor could directly detect genetically modified genes extracted from soybeans. We obtained two percentage calibration curves. The calibration curve corresponding to the lower percentage range (1–6%) exhibits a sensitivity of 2.36 Ω/% with R<sup>2</sup> = 0.9983, while the calibration curve corresponding to the higher percentage range (6–40%) possesses a sensitivity of 0.1 Ω/% with R<sup>2</sup> = 0.9928. The limit of detection would be 1%. The recovery rates for the 4% and 5.7% GMS DNA were measured to be 104.1% and 102.49% with RSD at 6.24% and 2.54%. The gold nanoparticle sensing electrode developed in this research is suitable for qualitative and quantitative detection of genetically modified soybeans and can be further applied to the detection of other genetically modified crops in the future.https://www.mdpi.com/2079-6374/12/4/207monolayer of gold nanoparticle sensing electrodeself-assembled monolayerelectrochemical impedance spectroscopygenetically modified soybeanslabel-free detection |
| spellingShingle | Cheng-Chi Chou Ying-Ting Lin Iren Kuznetsova Gou-Jen Wang Genetically Modified Soybean Detection Using a Biosensor Electrode with a Self-Assembled Monolayer of Gold Nanoparticles Biosensors monolayer of gold nanoparticle sensing electrode self-assembled monolayer electrochemical impedance spectroscopy genetically modified soybeans label-free detection |
| title | Genetically Modified Soybean Detection Using a Biosensor Electrode with a Self-Assembled Monolayer of Gold Nanoparticles |
| title_full | Genetically Modified Soybean Detection Using a Biosensor Electrode with a Self-Assembled Monolayer of Gold Nanoparticles |
| title_fullStr | Genetically Modified Soybean Detection Using a Biosensor Electrode with a Self-Assembled Monolayer of Gold Nanoparticles |
| title_full_unstemmed | Genetically Modified Soybean Detection Using a Biosensor Electrode with a Self-Assembled Monolayer of Gold Nanoparticles |
| title_short | Genetically Modified Soybean Detection Using a Biosensor Electrode with a Self-Assembled Monolayer of Gold Nanoparticles |
| title_sort | genetically modified soybean detection using a biosensor electrode with a self assembled monolayer of gold nanoparticles |
| topic | monolayer of gold nanoparticle sensing electrode self-assembled monolayer electrochemical impedance spectroscopy genetically modified soybeans label-free detection |
| url | https://www.mdpi.com/2079-6374/12/4/207 |
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