Gentisyl Alcohol Inhibits Proliferation and Induces Apoptosis via Mitochondrial Dysfunction and Regulation of MAPK and PI3K/AKT Pathways in Epithelial Ovarian Cancer Cells
Ovarian cancer is one of the prevalent gynecological cancers occurring in women. In particular, the efficiency of standard therapeutic methods decreases when recurrence and chemoresistance ensue. To assist standard anti-cancer agents in the cure of ovarian cancer, development and application of new...
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MDPI AG
2019-06-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/17/6/331 |
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| author | Jiyeon Ham Whasun Lim Kyeongwon Kim Young Mok Heo Seung Mok Ryu Dongho Lee Jae-Jin Kim Gwonhwa Song |
| author_facet | Jiyeon Ham Whasun Lim Kyeongwon Kim Young Mok Heo Seung Mok Ryu Dongho Lee Jae-Jin Kim Gwonhwa Song |
| author_sort | Jiyeon Ham |
| collection | DOAJ |
| description | Ovarian cancer is one of the prevalent gynecological cancers occurring in women. In particular, the efficiency of standard therapeutic methods decreases when recurrence and chemoresistance ensue. To assist standard anti-cancer agents in the cure of ovarian cancer, development and application of new compounds such as small molecules or natural products are required. Gentisyl alcohol is one of the secondary metabolites that can be obtained by purification from bacteria or fungi and is known to have antibacterial, antifungal, antiviral, and anti-cancer effects. In the present study, we verified the effect of gentisyl alcohol derived from marine <i>Arthrinium</i> sp. on suppressing proliferation and inducing apoptosis via DNA fragmentation in human ovarian cancers cells (ES2 and OV90 cells). We also confirmed that there was an accumulation of sub-G1 cells and a loss of mitochondrial membrane potential with calcium dysregulation in gentisyl alcohol-treated ovarian cancer cells. Moreover, gentisyl alcohol up-regulated signal transduction of MAPK and PI3K/AKT pathways. Collectively, our results demonstrated the possibility of gentisyl alcohol as a novel therapeutic agent for human ovarian cancer. |
| first_indexed | 2024-12-10T07:22:23Z |
| format | Article |
| id | doaj.art-1c853902ca6240c29c8bfe8e3747461c |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-12-10T07:22:23Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-1c853902ca6240c29c8bfe8e3747461c2022-12-22T01:57:46ZengMDPI AGMarine Drugs1660-33972019-06-0117633110.3390/md17060331md17060331Gentisyl Alcohol Inhibits Proliferation and Induces Apoptosis via Mitochondrial Dysfunction and Regulation of MAPK and PI3K/AKT Pathways in Epithelial Ovarian Cancer CellsJiyeon Ham0Whasun Lim1Kyeongwon Kim2Young Mok Heo3Seung Mok Ryu4Dongho Lee5Jae-Jin Kim6Gwonhwa Song7Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, KoreaDepartment of Food and Nutrition, College of Science and Technology, Kookmin University, Seoul 02707, KoreaDivision of Environmental Science & Ecological Engineering, College of Life Sciences & Biotechnology, Korea University, Seoul 02841, KoreaDivision of Environmental Science & Ecological Engineering, College of Life Sciences & Biotechnology, Korea University, Seoul 02841, KoreaHerbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju-si, Jeollanam-do 58245, KoreaDepartment of Biosystem and Biotechnology, College of Life Sciences & Biotechnology, Korea University, Seoul 02841, KoreaDivision of Environmental Science & Ecological Engineering, College of Life Sciences & Biotechnology, Korea University, Seoul 02841, KoreaDepartment of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, KoreaOvarian cancer is one of the prevalent gynecological cancers occurring in women. In particular, the efficiency of standard therapeutic methods decreases when recurrence and chemoresistance ensue. To assist standard anti-cancer agents in the cure of ovarian cancer, development and application of new compounds such as small molecules or natural products are required. Gentisyl alcohol is one of the secondary metabolites that can be obtained by purification from bacteria or fungi and is known to have antibacterial, antifungal, antiviral, and anti-cancer effects. In the present study, we verified the effect of gentisyl alcohol derived from marine <i>Arthrinium</i> sp. on suppressing proliferation and inducing apoptosis via DNA fragmentation in human ovarian cancers cells (ES2 and OV90 cells). We also confirmed that there was an accumulation of sub-G1 cells and a loss of mitochondrial membrane potential with calcium dysregulation in gentisyl alcohol-treated ovarian cancer cells. Moreover, gentisyl alcohol up-regulated signal transduction of MAPK and PI3K/AKT pathways. Collectively, our results demonstrated the possibility of gentisyl alcohol as a novel therapeutic agent for human ovarian cancer.https://www.mdpi.com/1660-3397/17/6/331ovarian cancergentisyl alcoholmitochondrial dysfunctioncalcium dysregulation |
| spellingShingle | Jiyeon Ham Whasun Lim Kyeongwon Kim Young Mok Heo Seung Mok Ryu Dongho Lee Jae-Jin Kim Gwonhwa Song Gentisyl Alcohol Inhibits Proliferation and Induces Apoptosis via Mitochondrial Dysfunction and Regulation of MAPK and PI3K/AKT Pathways in Epithelial Ovarian Cancer Cells Marine Drugs ovarian cancer gentisyl alcohol mitochondrial dysfunction calcium dysregulation |
| title | Gentisyl Alcohol Inhibits Proliferation and Induces Apoptosis via Mitochondrial Dysfunction and Regulation of MAPK and PI3K/AKT Pathways in Epithelial Ovarian Cancer Cells |
| title_full | Gentisyl Alcohol Inhibits Proliferation and Induces Apoptosis via Mitochondrial Dysfunction and Regulation of MAPK and PI3K/AKT Pathways in Epithelial Ovarian Cancer Cells |
| title_fullStr | Gentisyl Alcohol Inhibits Proliferation and Induces Apoptosis via Mitochondrial Dysfunction and Regulation of MAPK and PI3K/AKT Pathways in Epithelial Ovarian Cancer Cells |
| title_full_unstemmed | Gentisyl Alcohol Inhibits Proliferation and Induces Apoptosis via Mitochondrial Dysfunction and Regulation of MAPK and PI3K/AKT Pathways in Epithelial Ovarian Cancer Cells |
| title_short | Gentisyl Alcohol Inhibits Proliferation and Induces Apoptosis via Mitochondrial Dysfunction and Regulation of MAPK and PI3K/AKT Pathways in Epithelial Ovarian Cancer Cells |
| title_sort | gentisyl alcohol inhibits proliferation and induces apoptosis via mitochondrial dysfunction and regulation of mapk and pi3k akt pathways in epithelial ovarian cancer cells |
| topic | ovarian cancer gentisyl alcohol mitochondrial dysfunction calcium dysregulation |
| url | https://www.mdpi.com/1660-3397/17/6/331 |
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