Tetrahydropteridines possess antioxidant roles to guard against glucose-induced oxidative stress in Dictyostelium discoideum
Glucose effects on the vegetative growth of Dictyostelium discoideumAx2 were studied by examining oxidative stress and tetrahydropteridinesynthesis in cells cultured with different concentrations(0.5X, 7.7 g L-1; 1X, 15.4 g L-1; 2X, 30.8 g L-1) ofglucose. The growth rate was optimal in 1X cells (cel...
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Korean Society for Biochemistry and Molecular Biology
2013-02-01
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| Series: | BMB Reports |
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| Online Access: | http://bmbreports.org/jbmb/pdf.php?data=MTMwOTI2MTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0Ni0yJTVEMTMwMjI2MTgzMF8lMjgwODYtMDkxJTI5Qk1CXzEyLTEyOC5wZGY= |
| _version_ | 1817988707276292096 |
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| author | Seon-Ok Park Hye-Lim Kim Soo-Woong Lee Young Shik Park |
| author_facet | Seon-Ok Park Hye-Lim Kim Soo-Woong Lee Young Shik Park |
| author_sort | Seon-Ok Park |
| collection | DOAJ |
| description | Glucose effects on the vegetative growth of Dictyostelium discoideumAx2 were studied by examining oxidative stress and tetrahydropteridinesynthesis in cells cultured with different concentrations(0.5X, 7.7 g L-1; 1X, 15.4 g L-1; 2X, 30.8 g L-1) ofglucose. The growth rate was optimal in 1X cells (cells grown in1X glucose) but was impaired drastically in 2X cells, below thelevel of 0.5X cells. There were glucose-dependent increases inreactive oxygen species (ROS) levels and mitochondrial dysfunctionin parallel with the mRNA copy numbers of the enzymescatalyzing tetrahydropteridine synthesis and regeneration. Onthe other hand, both the specific activities of the enzymes andtetrahydropteridine levels in 2X cells were lower than those in1X cells, but were higher than those in 0.5X cells. Given the antioxidantfunction of tetrahydropteridines and both the beneficialand harmful effects of ROS, the results suggest glucose-inducedoxidative stress in Dictyostelium, a process that might originatefrom aerobic glycolysis, as well as a protective role of tetrahydropteridinesagainst this stress. [BMB Reports 2013; 46(2):86-91] |
| first_indexed | 2024-04-14T00:37:28Z |
| format | Article |
| id | doaj.art-1c8bd58d18714e54b495bf88fa94e32f |
| institution | Directory Open Access Journal |
| issn | 1976-6696 1976-670X |
| language | English |
| last_indexed | 2024-04-14T00:37:28Z |
| publishDate | 2013-02-01 |
| publisher | Korean Society for Biochemistry and Molecular Biology |
| record_format | Article |
| series | BMB Reports |
| spelling | doaj.art-1c8bd58d18714e54b495bf88fa94e32f2022-12-22T02:22:18ZengKorean Society for Biochemistry and Molecular BiologyBMB Reports1976-66961976-670X2013-02-014628691http://dx.doi.org/10.5483/BMBRep.2013.46.2.128Tetrahydropteridines possess antioxidant roles to guard against glucose-induced oxidative stress in Dictyostelium discoideumSeon-Ok ParkHye-Lim KimSoo-Woong LeeYoung Shik ParkGlucose effects on the vegetative growth of Dictyostelium discoideumAx2 were studied by examining oxidative stress and tetrahydropteridinesynthesis in cells cultured with different concentrations(0.5X, 7.7 g L-1; 1X, 15.4 g L-1; 2X, 30.8 g L-1) ofglucose. The growth rate was optimal in 1X cells (cells grown in1X glucose) but was impaired drastically in 2X cells, below thelevel of 0.5X cells. There were glucose-dependent increases inreactive oxygen species (ROS) levels and mitochondrial dysfunctionin parallel with the mRNA copy numbers of the enzymescatalyzing tetrahydropteridine synthesis and regeneration. Onthe other hand, both the specific activities of the enzymes andtetrahydropteridine levels in 2X cells were lower than those in1X cells, but were higher than those in 0.5X cells. Given the antioxidantfunction of tetrahydropteridines and both the beneficialand harmful effects of ROS, the results suggest glucose-inducedoxidative stress in Dictyostelium, a process that might originatefrom aerobic glycolysis, as well as a protective role of tetrahydropteridinesagainst this stress. [BMB Reports 2013; 46(2):86-91]http://bmbreports.org/jbmb/pdf.php?data=MTMwOTI2MTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0Ni0yJTVEMTMwMjI2MTgzMF8lMjgwODYtMDkxJTI5Qk1CXzEyLTEyOC5wZGY=DictyosteliumGlucoseMitochondriaOxidative stressTetrahydropteridines |
| spellingShingle | Seon-Ok Park Hye-Lim Kim Soo-Woong Lee Young Shik Park Tetrahydropteridines possess antioxidant roles to guard against glucose-induced oxidative stress in Dictyostelium discoideum BMB Reports Dictyostelium Glucose Mitochondria Oxidative stress Tetrahydropteridines |
| title | Tetrahydropteridines possess antioxidant roles to guard against glucose-induced oxidative stress in Dictyostelium discoideum |
| title_full | Tetrahydropteridines possess antioxidant roles to guard against glucose-induced oxidative stress in Dictyostelium discoideum |
| title_fullStr | Tetrahydropteridines possess antioxidant roles to guard against glucose-induced oxidative stress in Dictyostelium discoideum |
| title_full_unstemmed | Tetrahydropteridines possess antioxidant roles to guard against glucose-induced oxidative stress in Dictyostelium discoideum |
| title_short | Tetrahydropteridines possess antioxidant roles to guard against glucose-induced oxidative stress in Dictyostelium discoideum |
| title_sort | tetrahydropteridines possess antioxidant roles to guard against glucose induced oxidative stress in dictyostelium discoideum |
| topic | Dictyostelium Glucose Mitochondria Oxidative stress Tetrahydropteridines |
| url | http://bmbreports.org/jbmb/pdf.php?data=MTMwOTI2MTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0Ni0yJTVEMTMwMjI2MTgzMF8lMjgwODYtMDkxJTI5Qk1CXzEyLTEyOC5wZGY= |
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