Na<sup>+</sup>/K<sup>+</sup>- and Mg<sup>2+</sup>-ATPases and Their Interaction with AMPA, NMDA and D<sub>2</sub> Dopamine Receptors in an Animal Model of Febrile Seizures

Febrile seizures (FS) are one of the most common seizure disorders in childhood which are classified into short and prolonged, depending on their duration. Short FS are usually considered as benign. However, epidemiological studies have shown an association between prolonged FS and temporal lobe epi...

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Main Authors: María Crespo, David Agustín León-Navarro, Mairena Martín
Format: Article
Language:English
Published: MDPI AG 2022-11-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/23/23/14638
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author María Crespo
David Agustín León-Navarro
Mairena Martín
author_facet María Crespo
David Agustín León-Navarro
Mairena Martín
author_sort María Crespo
collection DOAJ
description Febrile seizures (FS) are one of the most common seizure disorders in childhood which are classified into short and prolonged, depending on their duration. Short FS are usually considered as benign. However, epidemiological studies have shown an association between prolonged FS and temporal lobe epilepsy. The development of animal models of FS has been very useful to investigate the mechanisms and the consequences of FS. One of the most used, the “hair dryer model”, has revealed that prolonged FS may lead to temporal lobe epilepsy by altering neuronal function. Several pieces of evidence suggest that Na<sup>+</sup>/ K<sup>+</sup>-ATPase and Mg<sup>2+</sup>-ATPase may play a role in this epileptogenic process. In this work, we found that hyperthermia-induced seizures (HIS) significantly increased the activity of Na<sup>+</sup>/ K<sup>+</sup>-ATPase and Mg<sup>2+</sup>-ATPase five and twenty days after hyperthermic insult, respectively. These effects were diminished in response to AMPA, D<sub>2</sub> dopamine A<sub>1</sub> and A<sub>2A</sub> receptors activation, respectively. Furthermore, HIS also significantly increased the protein level of the AMPA subunit GluR1. Altogether, the increased Na<sup>+</sup>/ K<sup>+</sup>-ATPase and Mg<sup>2+</sup>-ATPase agree well with the presence of protective mechanisms. However, the reduction in ATPase activities in the presence of NMDA and AMPA suggest an increased propensity for epileptic events in adults.
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spelling doaj.art-1c8e29800b404734b19cdf7fc9d0cc9b2023-11-24T11:05:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123231463810.3390/ijms232314638Na<sup>+</sup>/K<sup>+</sup>- and Mg<sup>2+</sup>-ATPases and Their Interaction with AMPA, NMDA and D<sub>2</sub> Dopamine Receptors in an Animal Model of Febrile SeizuresMaría Crespo0David Agustín León-Navarro1Mairena Martín2Department of Inorganic, Organic Chemistry and Biochemistry, Faculty of Chemical and Technological Sciences, Regional Centre of Biomedical Research (CRIB), Universidad de Castilla-La Mancha, 13071 Ciudad Real, SpainDepartment of Inorganic, Organic Chemistry and Biochemistry, Faculty of Chemical and Technological Sciences, Regional Centre of Biomedical Research (CRIB), Universidad de Castilla-La Mancha, 13071 Ciudad Real, SpainDepartment of Inorganic, Organic Chemistry and Biochemistry, Faculty of Chemical and Technological Sciences, School of Medicine of Ciudad Real, Regional Centre of Biomedical Research (CRIB), Universidad de Castilla-La Mancha, 13071 Ciudad Real, SpainFebrile seizures (FS) are one of the most common seizure disorders in childhood which are classified into short and prolonged, depending on their duration. Short FS are usually considered as benign. However, epidemiological studies have shown an association between prolonged FS and temporal lobe epilepsy. The development of animal models of FS has been very useful to investigate the mechanisms and the consequences of FS. One of the most used, the “hair dryer model”, has revealed that prolonged FS may lead to temporal lobe epilepsy by altering neuronal function. Several pieces of evidence suggest that Na<sup>+</sup>/ K<sup>+</sup>-ATPase and Mg<sup>2+</sup>-ATPase may play a role in this epileptogenic process. In this work, we found that hyperthermia-induced seizures (HIS) significantly increased the activity of Na<sup>+</sup>/ K<sup>+</sup>-ATPase and Mg<sup>2+</sup>-ATPase five and twenty days after hyperthermic insult, respectively. These effects were diminished in response to AMPA, D<sub>2</sub> dopamine A<sub>1</sub> and A<sub>2A</sub> receptors activation, respectively. Furthermore, HIS also significantly increased the protein level of the AMPA subunit GluR1. Altogether, the increased Na<sup>+</sup>/ K<sup>+</sup>-ATPase and Mg<sup>2+</sup>-ATPase agree well with the presence of protective mechanisms. However, the reduction in ATPase activities in the presence of NMDA and AMPA suggest an increased propensity for epileptic events in adults.https://www.mdpi.com/1422-0067/23/23/14638Na<sup>+</sup>/K<sup>+</sup> ATPaseMg<sup>2+</sup> ATPaseAMPA receptorNMDA receptorfebrile seizureepilepsy
spellingShingle María Crespo
David Agustín León-Navarro
Mairena Martín
Na<sup>+</sup>/K<sup>+</sup>- and Mg<sup>2+</sup>-ATPases and Their Interaction with AMPA, NMDA and D<sub>2</sub> Dopamine Receptors in an Animal Model of Febrile Seizures
International Journal of Molecular Sciences
Na<sup>+</sup>/K<sup>+</sup> ATPase
Mg<sup>2+</sup> ATPase
AMPA receptor
NMDA receptor
febrile seizure
epilepsy
title Na<sup>+</sup>/K<sup>+</sup>- and Mg<sup>2+</sup>-ATPases and Their Interaction with AMPA, NMDA and D<sub>2</sub> Dopamine Receptors in an Animal Model of Febrile Seizures
title_full Na<sup>+</sup>/K<sup>+</sup>- and Mg<sup>2+</sup>-ATPases and Their Interaction with AMPA, NMDA and D<sub>2</sub> Dopamine Receptors in an Animal Model of Febrile Seizures
title_fullStr Na<sup>+</sup>/K<sup>+</sup>- and Mg<sup>2+</sup>-ATPases and Their Interaction with AMPA, NMDA and D<sub>2</sub> Dopamine Receptors in an Animal Model of Febrile Seizures
title_full_unstemmed Na<sup>+</sup>/K<sup>+</sup>- and Mg<sup>2+</sup>-ATPases and Their Interaction with AMPA, NMDA and D<sub>2</sub> Dopamine Receptors in an Animal Model of Febrile Seizures
title_short Na<sup>+</sup>/K<sup>+</sup>- and Mg<sup>2+</sup>-ATPases and Their Interaction with AMPA, NMDA and D<sub>2</sub> Dopamine Receptors in an Animal Model of Febrile Seizures
title_sort na sup sup k sup sup and mg sup 2 sup atpases and their interaction with ampa nmda and d sub 2 sub dopamine receptors in an animal model of febrile seizures
topic Na<sup>+</sup>/K<sup>+</sup> ATPase
Mg<sup>2+</sup> ATPase
AMPA receptor
NMDA receptor
febrile seizure
epilepsy
url https://www.mdpi.com/1422-0067/23/23/14638
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AT davidagustinleonnavarro nasupsupksupsupandmgsup2supatpasesandtheirinteractionwithampanmdaanddsub2subdopaminereceptorsinananimalmodeloffebrileseizures
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