miRNA patterns in male LUSC patients - the 3-way mirror: Tissue, plasma and exosomes
Lung cancer remains one of the leading causes of cancer-related deaths worldwide. It is classified into two main histological groups: non-small cell lung cancer (NSCLC) and small cell lung cancer. Improving the outcome of cancer patients could be possible by enhancing the early diagnosis. In the cur...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-06-01
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| Series: | Translational Oncology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1936523324000780 |
| _version_ | 1797226674570395648 |
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| author | Cecilia Bica Ancuta Jurj Antonia Harangus Cristina Ciocan Alin Moldovan Oana Zanoaga Claudia Burz Manuela Ferracin Lajos Raduly Ioana Berindan-Neagoe |
| author_facet | Cecilia Bica Ancuta Jurj Antonia Harangus Cristina Ciocan Alin Moldovan Oana Zanoaga Claudia Burz Manuela Ferracin Lajos Raduly Ioana Berindan-Neagoe |
| author_sort | Cecilia Bica |
| collection | DOAJ |
| description | Lung cancer remains one of the leading causes of cancer-related deaths worldwide. It is classified into two main histological groups: non-small cell lung cancer (NSCLC) and small cell lung cancer. Improving the outcome of cancer patients could be possible by enhancing the early diagnosis. In the current study, we evaluated the levels of three microRNAs - miR-21-5p, miR-155-5p, and miR-181a-5p in tumor (TT) vs adjacent normal tissue (NT), as well as their expression levels in plasma and extracellular vesicles (EVs) from plasma in lung squamous cell carcinoma (LUSC) male patients vs healthy individuals as means to identify a panel of miRNAs that could serve as novel biomarkers for the diagnosis of LUSC in male patients. Matched paired tissue samples from male LUSC (n=40) patients were used for miRNA expression analysis. MiR-21-5p and miR-155-5p in tumor tissue were overexpressed, while underexpression of miR-181a-5p was observed in LUSC TT vs NT. These results were further validated in the TCGA LUSC dataset, considering 279 male samples. These alterations of miR-21-5p, miR-181a-5p, and miR-155-5p in tumor tissue are also present in plasma and plasma extracellular vesicles in LUSC male patients. In addition, ROC curves were performed to assess the sensitivity and specificity of different combinations of these miRNAs, confirming a high diagnostic accuracy for LUSC of up to 88 % in male subjects. The expression levels in tissue samples and the abundance in plasma and plasma EVs of the three miRNAs combined - miR-21-5p, miR-155-5p and miR-181a-5p – could be considered for further studies on biomarkers for the early detection of LUSC in male subjects. |
| first_indexed | 2024-04-24T14:28:40Z |
| format | Article |
| id | doaj.art-1c8e711548ee438e9c9b3b2f6fefc341 |
| institution | Directory Open Access Journal |
| issn | 1936-5233 |
| language | English |
| last_indexed | 2024-04-24T14:28:40Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj.art-1c8e711548ee438e9c9b3b2f6fefc3412024-04-03T04:26:22ZengElsevierTranslational Oncology1936-52332024-06-0144101951miRNA patterns in male LUSC patients - the 3-way mirror: Tissue, plasma and exosomesCecilia Bica0Ancuta Jurj1Antonia Harangus2Cristina Ciocan3Alin Moldovan4Oana Zanoaga5Claudia Burz6Manuela Ferracin7Lajos Raduly8Ioana Berindan-Neagoe9Research Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 400337, Romania; Research Center for Advanced Medicine-MedFUTURE, Department of Translational Medicine, ''Iuliu Haţieganu'' University of Medicine and Pharmacy, 400347 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 400337, RomaniaLeon Daniello Pulmonology Hospital, Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 400337, RomaniaLeon Daniello Pulmonology Hospital, Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 400337, RomaniaDepartment of Immunology and Allergology, Faculty of Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania; Department of Medical Oncology, The Oncology Institute ''Prof. Dr. Ion Chiricuţă'', 400015 Cluj-Napoca, RomaniaDepartment of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, ItalyResearch Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 400337, Romania; Corresponding author.Research Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, 400337, RomaniaLung cancer remains one of the leading causes of cancer-related deaths worldwide. It is classified into two main histological groups: non-small cell lung cancer (NSCLC) and small cell lung cancer. Improving the outcome of cancer patients could be possible by enhancing the early diagnosis. In the current study, we evaluated the levels of three microRNAs - miR-21-5p, miR-155-5p, and miR-181a-5p in tumor (TT) vs adjacent normal tissue (NT), as well as their expression levels in plasma and extracellular vesicles (EVs) from plasma in lung squamous cell carcinoma (LUSC) male patients vs healthy individuals as means to identify a panel of miRNAs that could serve as novel biomarkers for the diagnosis of LUSC in male patients. Matched paired tissue samples from male LUSC (n=40) patients were used for miRNA expression analysis. MiR-21-5p and miR-155-5p in tumor tissue were overexpressed, while underexpression of miR-181a-5p was observed in LUSC TT vs NT. These results were further validated in the TCGA LUSC dataset, considering 279 male samples. These alterations of miR-21-5p, miR-181a-5p, and miR-155-5p in tumor tissue are also present in plasma and plasma extracellular vesicles in LUSC male patients. In addition, ROC curves were performed to assess the sensitivity and specificity of different combinations of these miRNAs, confirming a high diagnostic accuracy for LUSC of up to 88 % in male subjects. The expression levels in tissue samples and the abundance in plasma and plasma EVs of the three miRNAs combined - miR-21-5p, miR-155-5p and miR-181a-5p – could be considered for further studies on biomarkers for the early detection of LUSC in male subjects.http://www.sciencedirect.com/science/article/pii/S1936523324000780Lung cancerBiomarkersExtracellular vesiclesmicroRNAs |
| spellingShingle | Cecilia Bica Ancuta Jurj Antonia Harangus Cristina Ciocan Alin Moldovan Oana Zanoaga Claudia Burz Manuela Ferracin Lajos Raduly Ioana Berindan-Neagoe miRNA patterns in male LUSC patients - the 3-way mirror: Tissue, plasma and exosomes Translational Oncology Lung cancer Biomarkers Extracellular vesicles microRNAs |
| title | miRNA patterns in male LUSC patients - the 3-way mirror: Tissue, plasma and exosomes |
| title_full | miRNA patterns in male LUSC patients - the 3-way mirror: Tissue, plasma and exosomes |
| title_fullStr | miRNA patterns in male LUSC patients - the 3-way mirror: Tissue, plasma and exosomes |
| title_full_unstemmed | miRNA patterns in male LUSC patients - the 3-way mirror: Tissue, plasma and exosomes |
| title_short | miRNA patterns in male LUSC patients - the 3-way mirror: Tissue, plasma and exosomes |
| title_sort | mirna patterns in male lusc patients the 3 way mirror tissue plasma and exosomes |
| topic | Lung cancer Biomarkers Extracellular vesicles microRNAs |
| url | http://www.sciencedirect.com/science/article/pii/S1936523324000780 |
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