Comparison of protective effects of electroacupuncture and moxibustion at Zusanli (ST 36) on perinatal nicotine exposure-induced lung phenotype in rat offspring
Objective: To compare the effects of electroacupuncture (EA) and moxibustion at Zusanli (ST 36) on the lung phenotype of rat offspring exposed to nicotine during the perinatal period. Methods: Pregnant Sprague-Dawley rats were randomly divided into 4 groups: the control group (saline only), the mode...
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Elsevier
2023-07-01
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| Series: | Journal of Traditional Chinese Medical Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095754823000364 |
| _version_ | 1797771545055068160 |
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| author | Yunpeng Ge Yitian Liu Guozhen Zhao Reiko Sakurai Yana Xie Tianyu Shi Yang Fang Jiajia Wang Virender K. Rehan Bo Ji |
| author_facet | Yunpeng Ge Yitian Liu Guozhen Zhao Reiko Sakurai Yana Xie Tianyu Shi Yang Fang Jiajia Wang Virender K. Rehan Bo Ji |
| author_sort | Yunpeng Ge |
| collection | DOAJ |
| description | Objective: To compare the effects of electroacupuncture (EA) and moxibustion at Zusanli (ST 36) on the lung phenotype of rat offspring exposed to nicotine during the perinatal period. Methods: Pregnant Sprague-Dawley rats were randomly divided into 4 groups: the control group (saline only), the model group (nicotine only), the EA group (nicotine + EA at ST 36 acupoints bilaterally), and the moxibustion group (nicotine + moxibustion at ST 36 acupoints bilaterally). n = 6 rats per group. On postnatal day 21, the body weight, lung weight, and pulmonary function were determined and lung morphometry was performed. Peroxisome proliferator-activated receptor gamma and β-catenin levels in the lung tissue of offspring were also determined. Results: Perinatal nicotine exposure (PNE) results in decreased body and lung weights of offspring rats, abnormal lung tissue morphology, and significantly altered pulmonary function, showing an increase in total airway resistance and a decrease in tidal volume, minute ventilation, total airway compliance, and peak expiratory flow. Bilateral EA at ST 36 acupoints could block all of these perinatal nicotine-induced effects. Although moxibustion also had protective effects in nicotine-induced offspring lungs, some of these effects did not reach statistical significance, e.g., protection against the upregulation of β-catenin, the downregulation of PPARγ signaling, and the increase in peak expiratory flow. Conclusion: Maternal EA at ST 36 blocked the PNE-induced changes in key developmental signaling pathways, prevented the PNE-induced changes in lung morphology, and protected pulmonary function. Moxibustion at ST 36 showed similar but weaker protective effects against the PNE-induced changes in the exposed offspring. It is important to note that the mechanism underlying the protective effects of moxibustion at ST 36 may be different from those of EA at ST 36, and further research is needed to understand these differences. |
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| institution | Directory Open Access Journal |
| issn | 2095-7548 |
| language | English |
| last_indexed | 2024-03-12T21:39:10Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
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| series | Journal of Traditional Chinese Medical Sciences |
| spelling | doaj.art-1c8f4301a10c40c0a80af3ac63f0b28c2023-07-27T05:55:43ZengElsevierJournal of Traditional Chinese Medical Sciences2095-75482023-07-01103362369Comparison of protective effects of electroacupuncture and moxibustion at Zusanli (ST 36) on perinatal nicotine exposure-induced lung phenotype in rat offspringYunpeng Ge0Yitian Liu1Guozhen Zhao2Reiko Sakurai3Yana Xie4Tianyu Shi5Yang Fang6Jiajia Wang7Virender K. Rehan8Bo Ji9School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 102488, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 102488, ChinaSchool of Clinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaLundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, David Geffen School of Medicine at UCLA, Los Angeles 90502, USASchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 102488, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 102488, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 102488, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 102488, ChinaLundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, David Geffen School of Medicine at UCLA, Los Angeles 90502, USASchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 102488, China; Corresponding author.Objective: To compare the effects of electroacupuncture (EA) and moxibustion at Zusanli (ST 36) on the lung phenotype of rat offspring exposed to nicotine during the perinatal period. Methods: Pregnant Sprague-Dawley rats were randomly divided into 4 groups: the control group (saline only), the model group (nicotine only), the EA group (nicotine + EA at ST 36 acupoints bilaterally), and the moxibustion group (nicotine + moxibustion at ST 36 acupoints bilaterally). n = 6 rats per group. On postnatal day 21, the body weight, lung weight, and pulmonary function were determined and lung morphometry was performed. Peroxisome proliferator-activated receptor gamma and β-catenin levels in the lung tissue of offspring were also determined. Results: Perinatal nicotine exposure (PNE) results in decreased body and lung weights of offspring rats, abnormal lung tissue morphology, and significantly altered pulmonary function, showing an increase in total airway resistance and a decrease in tidal volume, minute ventilation, total airway compliance, and peak expiratory flow. Bilateral EA at ST 36 acupoints could block all of these perinatal nicotine-induced effects. Although moxibustion also had protective effects in nicotine-induced offspring lungs, some of these effects did not reach statistical significance, e.g., protection against the upregulation of β-catenin, the downregulation of PPARγ signaling, and the increase in peak expiratory flow. Conclusion: Maternal EA at ST 36 blocked the PNE-induced changes in key developmental signaling pathways, prevented the PNE-induced changes in lung morphology, and protected pulmonary function. Moxibustion at ST 36 showed similar but weaker protective effects against the PNE-induced changes in the exposed offspring. It is important to note that the mechanism underlying the protective effects of moxibustion at ST 36 may be different from those of EA at ST 36, and further research is needed to understand these differences.http://www.sciencedirect.com/science/article/pii/S2095754823000364ElectroacupunctureMoxibustionPerinatal periodNicotine exposureLung developmentZusanli (ST 36) |
| spellingShingle | Yunpeng Ge Yitian Liu Guozhen Zhao Reiko Sakurai Yana Xie Tianyu Shi Yang Fang Jiajia Wang Virender K. Rehan Bo Ji Comparison of protective effects of electroacupuncture and moxibustion at Zusanli (ST 36) on perinatal nicotine exposure-induced lung phenotype in rat offspring Journal of Traditional Chinese Medical Sciences Electroacupuncture Moxibustion Perinatal period Nicotine exposure Lung development Zusanli (ST 36) |
| title | Comparison of protective effects of electroacupuncture and moxibustion at Zusanli (ST 36) on perinatal nicotine exposure-induced lung phenotype in rat offspring |
| title_full | Comparison of protective effects of electroacupuncture and moxibustion at Zusanli (ST 36) on perinatal nicotine exposure-induced lung phenotype in rat offspring |
| title_fullStr | Comparison of protective effects of electroacupuncture and moxibustion at Zusanli (ST 36) on perinatal nicotine exposure-induced lung phenotype in rat offspring |
| title_full_unstemmed | Comparison of protective effects of electroacupuncture and moxibustion at Zusanli (ST 36) on perinatal nicotine exposure-induced lung phenotype in rat offspring |
| title_short | Comparison of protective effects of electroacupuncture and moxibustion at Zusanli (ST 36) on perinatal nicotine exposure-induced lung phenotype in rat offspring |
| title_sort | comparison of protective effects of electroacupuncture and moxibustion at zusanli st 36 on perinatal nicotine exposure induced lung phenotype in rat offspring |
| topic | Electroacupuncture Moxibustion Perinatal period Nicotine exposure Lung development Zusanli (ST 36) |
| url | http://www.sciencedirect.com/science/article/pii/S2095754823000364 |
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