Isolation and Characterization of Bovine RVA from Northeast China, 2017–2020
Group A rotaviruses (RVAs) are major enteric pathogens causing infections in calves. To investigate the epidemiological characteristics and genetic diversity of bovine rotavirus (BRV), 233 fecal samples were collected from calves with diarrhea in northeast China. The samples were analyzed for sequen...
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MDPI AG
2021-12-01
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| Online Access: | https://www.mdpi.com/2075-1729/11/12/1389 |
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| author | Xi Cheng Wei Wu Fei Teng Yue Yan Guiwei Li Li Wang Xiaona Wang Ruichong Wang Han Zhou Yanping Jiang Wen Cui Lijie Tang Yijing Li Xinyuan Qiao |
| author_facet | Xi Cheng Wei Wu Fei Teng Yue Yan Guiwei Li Li Wang Xiaona Wang Ruichong Wang Han Zhou Yanping Jiang Wen Cui Lijie Tang Yijing Li Xinyuan Qiao |
| author_sort | Xi Cheng |
| collection | DOAJ |
| description | Group A rotaviruses (RVAs) are major enteric pathogens causing infections in calves. To investigate the epidemiological characteristics and genetic diversity of bovine rotavirus (BRV), 233 fecal samples were collected from calves with diarrhea in northeast China. The samples were analyzed for sequences encoding the inner capsid protein VP6 (subgroup) and the outer capsid proteins VP7 and VP4 (G and P type, respectively) using RT-PCR. Ten of the 233 samples (4.3%) were identified as BRV positive and were used for virus isolation and sequence analysis, revealing that all strains analyzed were of the G6P[1] genotype. The isolates exhibited high VP6 sequence identity to the USA cow RVA NCDV strain (>99% amino acid identity) and were further shown to be closely related to Japanese cow RVA BRV101 and Israelian human RVA G6P[1] strains, with >99% amino acid identity to VP7 and VP4 proteins, respectively. Comparative analyses of genome-predicted amino acid sequences between the isolates and the NCDV strains indicated that the antigenicity and infectivity of the strains isolated had changed. In this study, BRV genotypes and the genetic diversity among vaccinated cattle herds were monitored to provide epidemiological data and references for early diagnosis, allowing for early detection of new, potentially pathogenic RVA strains. |
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| language | English |
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| spelling | doaj.art-1c9072bf5dec456ea8ec1734d7a7e9e02023-11-23T09:14:45ZengMDPI AGLife2075-17292021-12-011112138910.3390/life11121389Isolation and Characterization of Bovine RVA from Northeast China, 2017–2020Xi Cheng0Wei Wu1Fei Teng2Yue Yan3Guiwei Li4Li Wang5Xiaona Wang6Ruichong Wang7Han Zhou8Yanping Jiang9Wen Cui10Lijie Tang11Yijing Li12Xinyuan Qiao13Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive, Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150038, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive, Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150038, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive, Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150038, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive, Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150038, ChinaBranch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161000, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive, Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150038, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive, Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150038, ChinaDepartment for Radiological Protection, Heilongjiang Province Center for Disease Control and Prevention, Harbin 150030, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive, Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150038, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive, Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150038, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive, Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150038, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive, Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150038, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive, Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150038, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive, Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150038, ChinaGroup A rotaviruses (RVAs) are major enteric pathogens causing infections in calves. To investigate the epidemiological characteristics and genetic diversity of bovine rotavirus (BRV), 233 fecal samples were collected from calves with diarrhea in northeast China. The samples were analyzed for sequences encoding the inner capsid protein VP6 (subgroup) and the outer capsid proteins VP7 and VP4 (G and P type, respectively) using RT-PCR. Ten of the 233 samples (4.3%) were identified as BRV positive and were used for virus isolation and sequence analysis, revealing that all strains analyzed were of the G6P[1] genotype. The isolates exhibited high VP6 sequence identity to the USA cow RVA NCDV strain (>99% amino acid identity) and were further shown to be closely related to Japanese cow RVA BRV101 and Israelian human RVA G6P[1] strains, with >99% amino acid identity to VP7 and VP4 proteins, respectively. Comparative analyses of genome-predicted amino acid sequences between the isolates and the NCDV strains indicated that the antigenicity and infectivity of the strains isolated had changed. In this study, BRV genotypes and the genetic diversity among vaccinated cattle herds were monitored to provide epidemiological data and references for early diagnosis, allowing for early detection of new, potentially pathogenic RVA strains.https://www.mdpi.com/2075-1729/11/12/1389bovine rotavirusepidemiological characteristicsvirus isolationsequence analysis |
| spellingShingle | Xi Cheng Wei Wu Fei Teng Yue Yan Guiwei Li Li Wang Xiaona Wang Ruichong Wang Han Zhou Yanping Jiang Wen Cui Lijie Tang Yijing Li Xinyuan Qiao Isolation and Characterization of Bovine RVA from Northeast China, 2017–2020 Life bovine rotavirus epidemiological characteristics virus isolation sequence analysis |
| title | Isolation and Characterization of Bovine RVA from Northeast China, 2017–2020 |
| title_full | Isolation and Characterization of Bovine RVA from Northeast China, 2017–2020 |
| title_fullStr | Isolation and Characterization of Bovine RVA from Northeast China, 2017–2020 |
| title_full_unstemmed | Isolation and Characterization of Bovine RVA from Northeast China, 2017–2020 |
| title_short | Isolation and Characterization of Bovine RVA from Northeast China, 2017–2020 |
| title_sort | isolation and characterization of bovine rva from northeast china 2017 2020 |
| topic | bovine rotavirus epidemiological characteristics virus isolation sequence analysis |
| url | https://www.mdpi.com/2075-1729/11/12/1389 |
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