SIP30 involvement in vesicle exocytosis from PC12 cells
SNAP25 (synaptosome-associated protein of 25 kDa) is a core SNARE (soluble N-ethylmaleimide-sensitive factor attachment receptor) protein; and the interaction between SNAP25 and other SNARE proteins is essential for synaptic vesicle exocytosis. Identified as a SNAP25 interacting protein, SIP30 (SNAP...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Biochemistry and Biophysics Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580823001954 |
| _version_ | 1827353617075535872 |
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| author | Ning Guo Lei Yu |
| author_facet | Ning Guo Lei Yu |
| author_sort | Ning Guo |
| collection | DOAJ |
| description | SNAP25 (synaptosome-associated protein of 25 kDa) is a core SNARE (soluble N-ethylmaleimide-sensitive factor attachment receptor) protein; and the interaction between SNAP25 and other SNARE proteins is essential for synaptic vesicle exocytosis. Identified as a SNAP25 interacting protein, SIP30 (SNAP25 interacting protein at 30 kDa) has been shown to modulate neuropathic pain behavior, and is potentially involved in the cellular process of vesicle exocytosis. Previous study demonstrated that using a vesicle secretion assay in PC12 cells, anti-SIP30 siRNA reduced vesicle exocytosis. We investigated vesicle exocytosis from PC12 cells with FM1-43 fluorescence dye, and demonstrated that anti-SIP30 siRNA reduced the pool of releasable vesicles and the rate of vesicle exocytosis, without affecting the endocytosis and recycling of the exocytosed vesicles. The results show that SIP30 is involved in vesicle exocytosis, suggesting a potential mechanism of SIP30 modulation of neuropathic pain. |
| first_indexed | 2024-03-08T03:30:01Z |
| format | Article |
| id | doaj.art-1c9100fcdadd463fb0fca6f616a073b8 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-03-08T03:30:01Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-1c9100fcdadd463fb0fca6f616a073b82024-02-11T05:11:15ZengElsevierBiochemistry and Biophysics Reports2405-58082024-03-0137101614SIP30 involvement in vesicle exocytosis from PC12 cellsNing Guo0Lei Yu1Department of Genetics, and Center of Alcohol & Substance Use Studies, Rutgers University, Piscataway, NJ, 08854, USACorresponding author.; Department of Genetics, and Center of Alcohol & Substance Use Studies, Rutgers University, Piscataway, NJ, 08854, USASNAP25 (synaptosome-associated protein of 25 kDa) is a core SNARE (soluble N-ethylmaleimide-sensitive factor attachment receptor) protein; and the interaction between SNAP25 and other SNARE proteins is essential for synaptic vesicle exocytosis. Identified as a SNAP25 interacting protein, SIP30 (SNAP25 interacting protein at 30 kDa) has been shown to modulate neuropathic pain behavior, and is potentially involved in the cellular process of vesicle exocytosis. Previous study demonstrated that using a vesicle secretion assay in PC12 cells, anti-SIP30 siRNA reduced vesicle exocytosis. We investigated vesicle exocytosis from PC12 cells with FM1-43 fluorescence dye, and demonstrated that anti-SIP30 siRNA reduced the pool of releasable vesicles and the rate of vesicle exocytosis, without affecting the endocytosis and recycling of the exocytosed vesicles. The results show that SIP30 is involved in vesicle exocytosis, suggesting a potential mechanism of SIP30 modulation of neuropathic pain.http://www.sciencedirect.com/science/article/pii/S2405580823001954SIP30ExocytosisSynaptic vesiclesPC12 cellsNeuropathic pain |
| spellingShingle | Ning Guo Lei Yu SIP30 involvement in vesicle exocytosis from PC12 cells Biochemistry and Biophysics Reports SIP30 Exocytosis Synaptic vesicles PC12 cells Neuropathic pain |
| title | SIP30 involvement in vesicle exocytosis from PC12 cells |
| title_full | SIP30 involvement in vesicle exocytosis from PC12 cells |
| title_fullStr | SIP30 involvement in vesicle exocytosis from PC12 cells |
| title_full_unstemmed | SIP30 involvement in vesicle exocytosis from PC12 cells |
| title_short | SIP30 involvement in vesicle exocytosis from PC12 cells |
| title_sort | sip30 involvement in vesicle exocytosis from pc12 cells |
| topic | SIP30 Exocytosis Synaptic vesicles PC12 cells Neuropathic pain |
| url | http://www.sciencedirect.com/science/article/pii/S2405580823001954 |
| work_keys_str_mv | AT ningguo sip30involvementinvesicleexocytosisfrompc12cells AT leiyu sip30involvementinvesicleexocytosisfrompc12cells |