Characterization of transitional memory CD4+ and CD8+ T-cell mobilization during and after an acute bout of exercise
T-cell subsets, including naïve (NA), central memory (CM), transitional memory (TM), effector memory (EM), and RA + effector memory (EMRA), differ in phenotype and function. T-cells are mobilized by exercise, with differences in the magnitude of mobilization between subsets. However, the response of...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Sports and Active Living |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fspor.2023.1120454/full |
| _version_ | 1827965881994444800 |
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| author | Rebekah M. Hunt Mahmoud T. Elzayat Melissa M. Markofski Mitzi Laughlin Emily C. LaVoy |
| author_facet | Rebekah M. Hunt Mahmoud T. Elzayat Melissa M. Markofski Mitzi Laughlin Emily C. LaVoy |
| author_sort | Rebekah M. Hunt |
| collection | DOAJ |
| description | T-cell subsets, including naïve (NA), central memory (CM), transitional memory (TM), effector memory (EM), and RA + effector memory (EMRA), differ in phenotype and function. T-cells are mobilized by exercise, with differences in the magnitude of mobilization between subsets. However, the response of TM T-cells to exercise has not yet been described. Further, T-cells expressing the late differentiation marker CD57 are known to be highly responsive to exercise, but the relative response of CD57 + and CD57- within T-cell subsets is unknown. We therefore aimed to characterize the exercise-induced mobilization of TM T-cells, as well as to compare the exercise response of CD57 + and CD57- cells within T-cell subsets.MethodsSeventeen participants (7 female; aged 18–40 years) cycled 30 min at 80% of their estimated maximum heart rate. Venous blood obtained pre, post, and 1H post-exercise was analyzed by flow cytometry. CD45RA, CCR7, and CD28 expression within CD4 + and CD8+ T-cells identified NA, CM, TM, EM, and EMRA subsets. CD57 expression within EM, EMRA, and CD28+ T-cells was also quantified. The relative mobilization of each subset was compared by calculating fold change in cell concentration during (ingress, post/pre) and after exercise (egress,1H post/post). Cytomegalovirus (CMV) serostatus was determined by ELISA and was considered in models.ResultsTM CD8+ T-cell concentration was greater post-exercise than pre-exercise (138.59 ± 56.42 cells/µl vs. 98.51 ± 39.68 cells/µl, p < 0.05), and the proportion of CD8 + with a TM phenotype was elevated 1H post-exercise (1H: 32.44 ± 10.38% vs. Pre: 30.15 ± 8.77%, p < 0.05). The relative mobilization during and after exercise of TM T-cells did not differ from NA and CM but was less than EM and EMRA subsets. Similar results were observed within CD4+ T-cells. CD57 + subsets of CD28+ T-cells and of EM and EMRA CD8+ T-cells exhibited a greater relative mobilization than CD57- subsets (all p < 0.05).ConclusionThese results indicate TM CD4 + and CD8+ T-cells are transiently mobilized into the blood with exercise, but not to as great of an extent as later differentiated EM and EMRA T-cells. Results also indicate CD57 identifies highly exercise responsive cells within CD8+ T-cell subsets. |
| first_indexed | 2024-04-09T17:41:24Z |
| format | Article |
| id | doaj.art-1c91cb2c4a4c4380a61355adf23c1daa |
| institution | Directory Open Access Journal |
| issn | 2624-9367 |
| language | English |
| last_indexed | 2024-04-09T17:41:24Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Sports and Active Living |
| spelling | doaj.art-1c91cb2c4a4c4380a61355adf23c1daa2023-04-17T04:50:09ZengFrontiers Media S.A.Frontiers in Sports and Active Living2624-93672023-04-01510.3389/fspor.2023.11204541120454Characterization of transitional memory CD4+ and CD8+ T-cell mobilization during and after an acute bout of exerciseRebekah M. Hunt0Mahmoud T. Elzayat1Melissa M. Markofski2Mitzi Laughlin3Emily C. LaVoy4Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, Houston, TX, United StatesLaboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, Houston, TX, United StatesLaboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, Houston, TX, United StatesHouston Methodist Orthopedics and Sports Medicine, Houston Methodist, Houston, TX, United StatesLaboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, Houston, TX, United StatesT-cell subsets, including naïve (NA), central memory (CM), transitional memory (TM), effector memory (EM), and RA + effector memory (EMRA), differ in phenotype and function. T-cells are mobilized by exercise, with differences in the magnitude of mobilization between subsets. However, the response of TM T-cells to exercise has not yet been described. Further, T-cells expressing the late differentiation marker CD57 are known to be highly responsive to exercise, but the relative response of CD57 + and CD57- within T-cell subsets is unknown. We therefore aimed to characterize the exercise-induced mobilization of TM T-cells, as well as to compare the exercise response of CD57 + and CD57- cells within T-cell subsets.MethodsSeventeen participants (7 female; aged 18–40 years) cycled 30 min at 80% of their estimated maximum heart rate. Venous blood obtained pre, post, and 1H post-exercise was analyzed by flow cytometry. CD45RA, CCR7, and CD28 expression within CD4 + and CD8+ T-cells identified NA, CM, TM, EM, and EMRA subsets. CD57 expression within EM, EMRA, and CD28+ T-cells was also quantified. The relative mobilization of each subset was compared by calculating fold change in cell concentration during (ingress, post/pre) and after exercise (egress,1H post/post). Cytomegalovirus (CMV) serostatus was determined by ELISA and was considered in models.ResultsTM CD8+ T-cell concentration was greater post-exercise than pre-exercise (138.59 ± 56.42 cells/µl vs. 98.51 ± 39.68 cells/µl, p < 0.05), and the proportion of CD8 + with a TM phenotype was elevated 1H post-exercise (1H: 32.44 ± 10.38% vs. Pre: 30.15 ± 8.77%, p < 0.05). The relative mobilization during and after exercise of TM T-cells did not differ from NA and CM but was less than EM and EMRA subsets. Similar results were observed within CD4+ T-cells. CD57 + subsets of CD28+ T-cells and of EM and EMRA CD8+ T-cells exhibited a greater relative mobilization than CD57- subsets (all p < 0.05).ConclusionThese results indicate TM CD4 + and CD8+ T-cells are transiently mobilized into the blood with exercise, but not to as great of an extent as later differentiated EM and EMRA T-cells. Results also indicate CD57 identifies highly exercise responsive cells within CD8+ T-cell subsets.https://www.frontiersin.org/articles/10.3389/fspor.2023.1120454/fulllymphocytesphysical activityleukocyte differentiationexercise responseimmunity |
| spellingShingle | Rebekah M. Hunt Mahmoud T. Elzayat Melissa M. Markofski Mitzi Laughlin Emily C. LaVoy Characterization of transitional memory CD4+ and CD8+ T-cell mobilization during and after an acute bout of exercise Frontiers in Sports and Active Living lymphocytes physical activity leukocyte differentiation exercise response immunity |
| title | Characterization of transitional memory CD4+ and CD8+ T-cell mobilization during and after an acute bout of exercise |
| title_full | Characterization of transitional memory CD4+ and CD8+ T-cell mobilization during and after an acute bout of exercise |
| title_fullStr | Characterization of transitional memory CD4+ and CD8+ T-cell mobilization during and after an acute bout of exercise |
| title_full_unstemmed | Characterization of transitional memory CD4+ and CD8+ T-cell mobilization during and after an acute bout of exercise |
| title_short | Characterization of transitional memory CD4+ and CD8+ T-cell mobilization during and after an acute bout of exercise |
| title_sort | characterization of transitional memory cd4 and cd8 t cell mobilization during and after an acute bout of exercise |
| topic | lymphocytes physical activity leukocyte differentiation exercise response immunity |
| url | https://www.frontiersin.org/articles/10.3389/fspor.2023.1120454/full |
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