Variation in denitrifying bacterial communities along a primary succession in the Hailuogou Glacier retreat area, China
Background The Hailuogou Glacier is located at the Gongga Mountain on the southeastern edge of the Tibetan Plateau, and has retreated continuously as a result of global warming. The retreat of the Hailuogou Glacier has left behind a primary succession along soil chronosequences. Hailuogou Glacier’s...
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PeerJ Inc.
2019-08-01
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| Online Access: | https://peerj.com/articles/7356.pdf |
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| author | Yan Bai Xiying Huang Xiangrui Zhou Quanju Xiang Ke Zhao Xiumei Yu Qiang Chen Hao Jiang Tashi Nyima Xue Gao Yunfu Gu |
| author_facet | Yan Bai Xiying Huang Xiangrui Zhou Quanju Xiang Ke Zhao Xiumei Yu Qiang Chen Hao Jiang Tashi Nyima Xue Gao Yunfu Gu |
| author_sort | Yan Bai |
| collection | DOAJ |
| description | Background The Hailuogou Glacier is located at the Gongga Mountain on the southeastern edge of the Tibetan Plateau, and has retreated continuously as a result of global warming. The retreat of the Hailuogou Glacier has left behind a primary succession along soil chronosequences. Hailuogou Glacier’s retreated area provides an excellent living environment for the colonization of microbes and plants, making it an ideal model to explore plant successions, microbial communities, and the interaction of plants and microbes during the colonization process. However, to date, the density of the nitrogen cycling microbial communities remain unknown, especially for denitrifiers in the primary succession of the Hailuogou Glacier. Therefore, we investigated the structural succession and its driving factors for denitrifying bacterial communities during the four successional stages (0, 20, 40, and 60 years). Methods The diversity, community composition, and abundance of nosZ-denitrifiers were determined using molecular tools, including terminal restriction fragment length polymorphism and quantitative polymerase chain reactions (qPCR). Results nosZ-denitrifiers were more abundant and diverse in soils from successional years 20–60 compared to 0–5 years, and was highest in Site3 (40 years). The denitrifying bacterial community composition was more complex in older soils (40–60 years) than in younger soils (≤20 years). The terminal restriction fragments (T-RFs) of Azospirillum (90 bp) and Rubrivivax (95 bp) were dominant in soisl during early successional stages (0–20 years) and in the mature phase (40–60 years), respectively. Specific T-RFs of Bradyrhizobium (100 bp) and Pseudomonas (275 bp) were detected only in Site3 and Site4, respectively. Moreover, the unidentified 175 bp T-RFs was detected only in Site3. Of the abiotic factors that were measured in this study, soil available phosphorus, available potassium and denitrifying enzyme activity (DEA) correlated significantly with the community composition of nosZ-denitrifiers (P < 0.05 by Monte Carlo permutation test within RDA analysis). |
| first_indexed | 2024-03-09T07:23:39Z |
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| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:23:39Z |
| publishDate | 2019-08-01 |
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| spelling | doaj.art-1c925c516e5b46989559623237af8d322023-12-03T07:09:18ZengPeerJ Inc.PeerJ2167-83592019-08-017e735610.7717/peerj.7356Variation in denitrifying bacterial communities along a primary succession in the Hailuogou Glacier retreat area, ChinaYan Bai0Xiying Huang1Xiangrui Zhou2Quanju Xiang3Ke Zhao4Xiumei Yu5Qiang Chen6Hao Jiang7Tashi Nyima8Xue Gao9Yunfu Gu10Department of Microbiology/ College of Resources/Sichuan Agricultural University, Sichuan Agricultural University, Chengdu, Sichuan, ChinaDepartment of Microbiology/ College of Resources/Sichuan Agricultural University, Sichuan Agricultural University, Chengdu, Sichuan, ChinaDepartment of Microbiology/ College of Resources/Sichuan Agricultural University, Sichuan Agricultural University, Chengdu, Sichuan, ChinaDepartment of Microbiology/ College of Resources/Sichuan Agricultural University, Sichuan Agricultural University, Chengdu, Sichuan, ChinaDepartment of Microbiology/ College of Resources/Sichuan Agricultural University, Sichuan Agricultural University, Chengdu, Sichuan, ChinaDepartment of Microbiology/ College of Resources/Sichuan Agricultural University, Sichuan Agricultural University, Chengdu, Sichuan, ChinaDepartment of Microbiology/ College of Resources/Sichuan Agricultural University, Sichuan Agricultural University, Chengdu, Sichuan, ChinaInstitute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu, Sichuan, ChinaInstitute of Agricultural Resources and Environmental Science, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, Tibet, ChinaInstitute of Agricultural Resources and Environmental Science, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, Tibet, ChinaDepartment of Microbiology/ College of Resources/Sichuan Agricultural University, Sichuan Agricultural University, Chengdu, Sichuan, ChinaBackground The Hailuogou Glacier is located at the Gongga Mountain on the southeastern edge of the Tibetan Plateau, and has retreated continuously as a result of global warming. The retreat of the Hailuogou Glacier has left behind a primary succession along soil chronosequences. Hailuogou Glacier’s retreated area provides an excellent living environment for the colonization of microbes and plants, making it an ideal model to explore plant successions, microbial communities, and the interaction of plants and microbes during the colonization process. However, to date, the density of the nitrogen cycling microbial communities remain unknown, especially for denitrifiers in the primary succession of the Hailuogou Glacier. Therefore, we investigated the structural succession and its driving factors for denitrifying bacterial communities during the four successional stages (0, 20, 40, and 60 years). Methods The diversity, community composition, and abundance of nosZ-denitrifiers were determined using molecular tools, including terminal restriction fragment length polymorphism and quantitative polymerase chain reactions (qPCR). Results nosZ-denitrifiers were more abundant and diverse in soils from successional years 20–60 compared to 0–5 years, and was highest in Site3 (40 years). The denitrifying bacterial community composition was more complex in older soils (40–60 years) than in younger soils (≤20 years). The terminal restriction fragments (T-RFs) of Azospirillum (90 bp) and Rubrivivax (95 bp) were dominant in soisl during early successional stages (0–20 years) and in the mature phase (40–60 years), respectively. Specific T-RFs of Bradyrhizobium (100 bp) and Pseudomonas (275 bp) were detected only in Site3 and Site4, respectively. Moreover, the unidentified 175 bp T-RFs was detected only in Site3. Of the abiotic factors that were measured in this study, soil available phosphorus, available potassium and denitrifying enzyme activity (DEA) correlated significantly with the community composition of nosZ-denitrifiers (P < 0.05 by Monte Carlo permutation test within RDA analysis).https://peerj.com/articles/7356.pdfHailuogou Glacier retreat areaPrimary successionDenitrifying bacteriaCommunityAbundance |
| spellingShingle | Yan Bai Xiying Huang Xiangrui Zhou Quanju Xiang Ke Zhao Xiumei Yu Qiang Chen Hao Jiang Tashi Nyima Xue Gao Yunfu Gu Variation in denitrifying bacterial communities along a primary succession in the Hailuogou Glacier retreat area, China PeerJ Hailuogou Glacier retreat area Primary succession Denitrifying bacteria Community Abundance |
| title | Variation in denitrifying bacterial communities along a primary succession in the Hailuogou Glacier retreat area, China |
| title_full | Variation in denitrifying bacterial communities along a primary succession in the Hailuogou Glacier retreat area, China |
| title_fullStr | Variation in denitrifying bacterial communities along a primary succession in the Hailuogou Glacier retreat area, China |
| title_full_unstemmed | Variation in denitrifying bacterial communities along a primary succession in the Hailuogou Glacier retreat area, China |
| title_short | Variation in denitrifying bacterial communities along a primary succession in the Hailuogou Glacier retreat area, China |
| title_sort | variation in denitrifying bacterial communities along a primary succession in the hailuogou glacier retreat area china |
| topic | Hailuogou Glacier retreat area Primary succession Denitrifying bacteria Community Abundance |
| url | https://peerj.com/articles/7356.pdf |
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