Cloning and Phylogenetic Relationships Analysis of an Oxidoreductase Gene in Product Pathway of Ethylene Hormone
1-aminocyclopropane-1-carboxylic acid oxidase (<em>ACO</em>) enzyme is a member of the Fe (II-dependent family of oxidases/ oxygenases) and for activity requires Fe<sup>2+</sup> as cofactors and ascorbate as a co-substrate. This enzyme is the only oxidoreductase in the ethyle...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | fas |
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Shahid Bahonar University of Kerman
2015-11-01
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| Series: | مجله بیوتکنولوژی کشاورزی |
| Subjects: | |
| Online Access: | https://jab.uk.ac.ir/article_1132_0bc776ba55f1c2ed4dbd6dc2f19eb58f.pdf |
| _version_ | 1818304578386395136 |
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| author | Zohreh Jafari Rahim Haddad Ramin Hosseini Qasem Ali Garousi |
| author_facet | Zohreh Jafari Rahim Haddad Ramin Hosseini Qasem Ali Garousi |
| author_sort | Zohreh Jafari |
| collection | DOAJ |
| description | 1-aminocyclopropane-1-carboxylic acid oxidase (<em>ACO</em>) enzyme is a member of the Fe (II-dependent family of oxidases/ oxygenases) and for activity requires Fe<sup>2+</sup> as cofactors and ascorbate as a co-substrate. This enzyme is the only oxidoreductase in the ethylene biosynthesis pathway and catalysis the biosynthesis terminal step. In the present study, total RNA was extracted from tomato fruit (cv, Memory I), and then, coding cDNA of the <em>LeACO1</em> gene was synthesized by reverse transcription polymerase chain reaction (RT-PCR). The PCR product was cloned into a pUC19 plasmid, and then, their biochemical, structural and phylogenetic characteristics were analyzed. LeACO1 amino acid sequence studies in tomato and other plant of such family were indicated that His177, His234 and Asp179 amino acids are formed a triangle catalytically site to play a role in binding Fe<sup>2+</sup> cofactor. Phylogenetic and multiple alignment analysis were illustrated a high degree of identity to those of the <em>ACO1</em> gene from other plants. |
| first_indexed | 2024-12-13T06:12:55Z |
| format | Article |
| id | doaj.art-1c92e25af2da467d9ea6d2afad6ad592 |
| institution | Directory Open Access Journal |
| issn | 2228-6705 2228-6500 |
| language | fas |
| last_indexed | 2024-12-13T06:12:55Z |
| publishDate | 2015-11-01 |
| publisher | Shahid Bahonar University of Kerman |
| record_format | Article |
| series | مجله بیوتکنولوژی کشاورزی |
| spelling | doaj.art-1c92e25af2da467d9ea6d2afad6ad5922022-12-21T23:57:02ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002015-11-0173557410.22103/jab.2015.11321132Cloning and Phylogenetic Relationships Analysis of an Oxidoreductase Gene in Product Pathway of Ethylene HormoneZohreh JafariRahim HaddadRamin HosseiniQasem Ali Garousi1-aminocyclopropane-1-carboxylic acid oxidase (<em>ACO</em>) enzyme is a member of the Fe (II-dependent family of oxidases/ oxygenases) and for activity requires Fe<sup>2+</sup> as cofactors and ascorbate as a co-substrate. This enzyme is the only oxidoreductase in the ethylene biosynthesis pathway and catalysis the biosynthesis terminal step. In the present study, total RNA was extracted from tomato fruit (cv, Memory I), and then, coding cDNA of the <em>LeACO1</em> gene was synthesized by reverse transcription polymerase chain reaction (RT-PCR). The PCR product was cloned into a pUC19 plasmid, and then, their biochemical, structural and phylogenetic characteristics were analyzed. LeACO1 amino acid sequence studies in tomato and other plant of such family were indicated that His177, His234 and Asp179 amino acids are formed a triangle catalytically site to play a role in binding Fe<sup>2+</sup> cofactor. Phylogenetic and multiple alignment analysis were illustrated a high degree of identity to those of the <em>ACO1</em> gene from other plants.https://jab.uk.ac.ir/article_1132_0bc776ba55f1c2ed4dbd6dc2f19eb58f.pdfactive siteaco1cloningoxidoreductasetomato |
| spellingShingle | Zohreh Jafari Rahim Haddad Ramin Hosseini Qasem Ali Garousi Cloning and Phylogenetic Relationships Analysis of an Oxidoreductase Gene in Product Pathway of Ethylene Hormone مجله بیوتکنولوژی کشاورزی active site aco1 cloning oxidoreductase tomato |
| title | Cloning and Phylogenetic Relationships Analysis of an Oxidoreductase Gene in Product Pathway of Ethylene Hormone |
| title_full | Cloning and Phylogenetic Relationships Analysis of an Oxidoreductase Gene in Product Pathway of Ethylene Hormone |
| title_fullStr | Cloning and Phylogenetic Relationships Analysis of an Oxidoreductase Gene in Product Pathway of Ethylene Hormone |
| title_full_unstemmed | Cloning and Phylogenetic Relationships Analysis of an Oxidoreductase Gene in Product Pathway of Ethylene Hormone |
| title_short | Cloning and Phylogenetic Relationships Analysis of an Oxidoreductase Gene in Product Pathway of Ethylene Hormone |
| title_sort | cloning and phylogenetic relationships analysis of an oxidoreductase gene in product pathway of ethylene hormone |
| topic | active site aco1 cloning oxidoreductase tomato |
| url | https://jab.uk.ac.ir/article_1132_0bc776ba55f1c2ed4dbd6dc2f19eb58f.pdf |
| work_keys_str_mv | AT zohrehjafari cloningandphylogeneticrelationshipsanalysisofanoxidoreductasegeneinproductpathwayofethylenehormone AT rahimhaddad cloningandphylogeneticrelationshipsanalysisofanoxidoreductasegeneinproductpathwayofethylenehormone AT raminhosseini cloningandphylogeneticrelationshipsanalysisofanoxidoreductasegeneinproductpathwayofethylenehormone AT qasemaligarousi cloningandphylogeneticrelationshipsanalysisofanoxidoreductasegeneinproductpathwayofethylenehormone |