BoMyrosinase plays an essential role in sulforaphane accumulation in response to selenite treatment in broccoli
Sulforaphane, a naturally specialized metabolite, plays significant roles in human disease prevention and plant defense. Myrosinase (MY) is a key gene responsible for the catalysis of sulforaphane formation, but the molecular mechanisms through which MY regulates sulforaphane biosynthesis in plants...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-03-01
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| Series: | Horticultural Plant Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014123000663 |
| _version_ | 1797271303117340672 |
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| author | Qiuyun Wu Qi Wu Yuxiao Tian Chunyan Zhou Shuxiang Mao Junwei Wang Ke Huang |
| author_facet | Qiuyun Wu Qi Wu Yuxiao Tian Chunyan Zhou Shuxiang Mao Junwei Wang Ke Huang |
| author_sort | Qiuyun Wu |
| collection | DOAJ |
| description | Sulforaphane, a naturally specialized metabolite, plays significant roles in human disease prevention and plant defense. Myrosinase (MY) is a key gene responsible for the catalysis of sulforaphane formation, but the molecular mechanisms through which MY regulates sulforaphane biosynthesis in plants remains largely unknown. Here, we discovered that the change of sulforaphane content in broccoli sprouts caused by exogenous selenite treatments is positively related to BoMY expression. BoMY overexpression in the Arabidopsis thaliana tgg1 mutants could dramatically increase myrosinase activity and sulforaphane content in the rosette leaves of 35S::BoMY/tgg1 and rescue its phenotypes. Moreover, an obvious increase of myrosinase activity and sulforaphane content was displayed in transgenic BoMY-overexpressed broccoli lines. In addition, a 2 033 bp promoter fragment of BoMY was isolated. Yeast one-hybrid (Y1H) library screening experiment uncovered that one bHLH transcription factor, BoFAMA, could directly bind to BoMY promoter to activate its expression, which was further evidenced by Y1H assay and dual-luciferase reporter assay. BoFAMA is a selenite-responsive transcription factor that is highly expressed in broccoli leaves; its protein is solely localized to nucleus. Additionally, genetic evidence suggested that the knockdown of FAMA gene in Arabidopsis thaliana could significantly decrease sulforaphane yield by inhibiting the expression of myrosinase genes. Interestingly, exogenous selenite supply could partially restore the low level of sulforaphane content in transgenic Arabidopsis FAMA-silencing plants. Our findings uncover a novel function of FAMA-MY module in the regulation of selenite-mediated sulforaphane synthesis and provide a new insights into the molecular mechanism by which selenite regulates the accumulation of sulforaphane in plants. |
| first_indexed | 2024-03-07T14:00:46Z |
| format | Article |
| id | doaj.art-1c94383fb4744d518f61e193a77b65a4 |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-03-07T14:00:46Z |
| publishDate | 2024-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj.art-1c94383fb4744d518f61e193a77b65a42024-03-07T05:28:47ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412024-03-01102488502BoMyrosinase plays an essential role in sulforaphane accumulation in response to selenite treatment in broccoliQiuyun Wu0Qi Wu1Yuxiao Tian2Chunyan Zhou3Shuxiang Mao4Junwei Wang5Ke Huang6College of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China; Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Changsha, Hunan 410128, China; Key Laboratory for Vegetable Biology of Hunan Province, Changsha, Hunan 410128, China; Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Changsha, Hunan 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China; Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Changsha, Hunan 410128, China; Key Laboratory for Vegetable Biology of Hunan Province, Changsha, Hunan 410128, China; Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Changsha, Hunan 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China; Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Changsha, Hunan 410128, China; Key Laboratory for Vegetable Biology of Hunan Province, Changsha, Hunan 410128, China; Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Changsha, Hunan 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China; Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Changsha, Hunan 410128, China; Key Laboratory for Vegetable Biology of Hunan Province, Changsha, Hunan 410128, China; Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Changsha, Hunan 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China; Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Changsha, Hunan 410128, China; Key Laboratory for Vegetable Biology of Hunan Province, Changsha, Hunan 410128, China; Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Changsha, Hunan 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China; Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Changsha, Hunan 410128, China; Key Laboratory for Vegetable Biology of Hunan Province, Changsha, Hunan 410128, China; Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Changsha, Hunan 410128, China; Corresponding authors.College of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China; Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Changsha, Hunan 410128, China; Key Laboratory for Vegetable Biology of Hunan Province, Changsha, Hunan 410128, China; Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Changsha, Hunan 410128, China; Corresponding authors.Sulforaphane, a naturally specialized metabolite, plays significant roles in human disease prevention and plant defense. Myrosinase (MY) is a key gene responsible for the catalysis of sulforaphane formation, but the molecular mechanisms through which MY regulates sulforaphane biosynthesis in plants remains largely unknown. Here, we discovered that the change of sulforaphane content in broccoli sprouts caused by exogenous selenite treatments is positively related to BoMY expression. BoMY overexpression in the Arabidopsis thaliana tgg1 mutants could dramatically increase myrosinase activity and sulforaphane content in the rosette leaves of 35S::BoMY/tgg1 and rescue its phenotypes. Moreover, an obvious increase of myrosinase activity and sulforaphane content was displayed in transgenic BoMY-overexpressed broccoli lines. In addition, a 2 033 bp promoter fragment of BoMY was isolated. Yeast one-hybrid (Y1H) library screening experiment uncovered that one bHLH transcription factor, BoFAMA, could directly bind to BoMY promoter to activate its expression, which was further evidenced by Y1H assay and dual-luciferase reporter assay. BoFAMA is a selenite-responsive transcription factor that is highly expressed in broccoli leaves; its protein is solely localized to nucleus. Additionally, genetic evidence suggested that the knockdown of FAMA gene in Arabidopsis thaliana could significantly decrease sulforaphane yield by inhibiting the expression of myrosinase genes. Interestingly, exogenous selenite supply could partially restore the low level of sulforaphane content in transgenic Arabidopsis FAMA-silencing plants. Our findings uncover a novel function of FAMA-MY module in the regulation of selenite-mediated sulforaphane synthesis and provide a new insights into the molecular mechanism by which selenite regulates the accumulation of sulforaphane in plants.http://www.sciencedirect.com/science/article/pii/S2468014123000663Brassica oleracea var. italicaMyrosinaseBoMYSulforaphaneSelenite treatment |
| spellingShingle | Qiuyun Wu Qi Wu Yuxiao Tian Chunyan Zhou Shuxiang Mao Junwei Wang Ke Huang BoMyrosinase plays an essential role in sulforaphane accumulation in response to selenite treatment in broccoli Horticultural Plant Journal Brassica oleracea var. italica Myrosinase BoMY Sulforaphane Selenite treatment |
| title | BoMyrosinase plays an essential role in sulforaphane accumulation in response to selenite treatment in broccoli |
| title_full | BoMyrosinase plays an essential role in sulforaphane accumulation in response to selenite treatment in broccoli |
| title_fullStr | BoMyrosinase plays an essential role in sulforaphane accumulation in response to selenite treatment in broccoli |
| title_full_unstemmed | BoMyrosinase plays an essential role in sulforaphane accumulation in response to selenite treatment in broccoli |
| title_short | BoMyrosinase plays an essential role in sulforaphane accumulation in response to selenite treatment in broccoli |
| title_sort | bomyrosinase plays an essential role in sulforaphane accumulation in response to selenite treatment in broccoli |
| topic | Brassica oleracea var. italica Myrosinase BoMY Sulforaphane Selenite treatment |
| url | http://www.sciencedirect.com/science/article/pii/S2468014123000663 |
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