Bilayer vascular grafts with on-demand NO and H2S release capabilities
Nitric oxide (NO) and hydrogen sulfide (H2S) gasotransmitters exhibit potential therapeutic effects in the cardiovascular system. Herein, biomimicking multilayer structures of biological blood vessels, bilayer small-diameter vascular grafts (SDVGs) with on-demand NO and H2S release capabilities, wer...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2024-01-01
|
| Series: | Bioactive Materials |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X23002335 |
| _version_ | 1797749085107650560 |
|---|---|
| author | Pengfei Li Fubang Liang Lijuan Wang Dawei Jin Yushuang Shang Xu Liu Yanjun Pan Jiang Yuan Jian Shen Meng Yin |
| author_facet | Pengfei Li Fubang Liang Lijuan Wang Dawei Jin Yushuang Shang Xu Liu Yanjun Pan Jiang Yuan Jian Shen Meng Yin |
| author_sort | Pengfei Li |
| collection | DOAJ |
| description | Nitric oxide (NO) and hydrogen sulfide (H2S) gasotransmitters exhibit potential therapeutic effects in the cardiovascular system. Herein, biomimicking multilayer structures of biological blood vessels, bilayer small-diameter vascular grafts (SDVGs) with on-demand NO and H2S release capabilities, were designed and fabricated. The keratin-based H2S donor (KTC) with good biocompatibility and high stability was first synthesized and then electrospun with poly (l-lactide-co-caprolactone) (PLCL) to be used as the outer layer of grafts. The electrospun poly (ε-caprolactone) (PCL) mats were aminolyzed and further chelated with copper (II) ions to construct glutathione peroxidase (GPx)-like structural surfaces for the catalytic generation of NO, which acted as the inner layer of grafts. The on-demand release of NO and H2S selectively and synergistically promoted the proliferation and migration of human umbilical vein endothelial cells (HUVECs) while inhibiting the proliferation and migration of human umbilical artery smooth muscle cells (HUASMCs). Dual releases of NO and H2S gasotransmitters could enhance their respective production, resulting in enhanced promotion of HUVECs and inhibition of HUASMCs owing to their combined actions. In addition, the bilayer grafts were conducive to forming endothelial cell layers under flow shear stress. In rat abdominal aorta replacement models, the grafts remained patency for 6 months. These grafts were capable of facilitating rapid endothelialization and alleviating neointimal hyperplasia without obvious injury, inflammation, or thrombosis. More importantly, the grafts were expected to avoid calcification with the degradation of the grafts. Taken together, these bilayer grafts will be greatly promising candidates for SDVGs with rapid endothelialization and anti-calcification properties. |
| first_indexed | 2024-03-12T16:15:11Z |
| format | Article |
| id | doaj.art-1c9527f9a77745e8b954abd5a5e19f87 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-03-12T16:15:11Z |
| publishDate | 2024-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-1c9527f9a77745e8b954abd5a5e19f872023-08-09T04:33:25ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2024-01-01313852Bilayer vascular grafts with on-demand NO and H2S release capabilitiesPengfei Li0Fubang Liang1Lijuan Wang2Dawei Jin3Yushuang Shang4Xu Liu5Yanjun Pan6Jiang Yuan7Jian Shen8Meng Yin9Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Bio-functional Materials, Department of Materials Science and Engineering, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, PR ChinaDepartment of Cardiothoracic Surgery, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, 1678 Dong Fang Road, Shanghai, 200127, PR ChinaJiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Bio-functional Materials, Department of Materials Science and Engineering, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, PR ChinaDepartment of Cardiothoracic Surgery, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, 1678 Dong Fang Road, Shanghai, 200127, PR ChinaJiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Bio-functional Materials, Department of Materials Science and Engineering, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, PR ChinaJiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Bio-functional Materials, Department of Materials Science and Engineering, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, PR ChinaDepartment of Cardiothoracic Surgery, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, 1678 Dong Fang Road, Shanghai, 200127, PR ChinaJiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Bio-functional Materials, Department of Materials Science and Engineering, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, PR China; Corresponding author.Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Bio-functional Materials, Department of Materials Science and Engineering, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, PR China; Jiangsu Engineering Research Center of Interfacial Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, PR China; Corresponding author. Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Bio-functional Materials, Department of Materials Science and Engineering, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, PR China.Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, 1678 Dong Fang Road, Shanghai, 200127, PR China; Corresponding author.Nitric oxide (NO) and hydrogen sulfide (H2S) gasotransmitters exhibit potential therapeutic effects in the cardiovascular system. Herein, biomimicking multilayer structures of biological blood vessels, bilayer small-diameter vascular grafts (SDVGs) with on-demand NO and H2S release capabilities, were designed and fabricated. The keratin-based H2S donor (KTC) with good biocompatibility and high stability was first synthesized and then electrospun with poly (l-lactide-co-caprolactone) (PLCL) to be used as the outer layer of grafts. The electrospun poly (ε-caprolactone) (PCL) mats were aminolyzed and further chelated with copper (II) ions to construct glutathione peroxidase (GPx)-like structural surfaces for the catalytic generation of NO, which acted as the inner layer of grafts. The on-demand release of NO and H2S selectively and synergistically promoted the proliferation and migration of human umbilical vein endothelial cells (HUVECs) while inhibiting the proliferation and migration of human umbilical artery smooth muscle cells (HUASMCs). Dual releases of NO and H2S gasotransmitters could enhance their respective production, resulting in enhanced promotion of HUVECs and inhibition of HUASMCs owing to their combined actions. In addition, the bilayer grafts were conducive to forming endothelial cell layers under flow shear stress. In rat abdominal aorta replacement models, the grafts remained patency for 6 months. These grafts were capable of facilitating rapid endothelialization and alleviating neointimal hyperplasia without obvious injury, inflammation, or thrombosis. More importantly, the grafts were expected to avoid calcification with the degradation of the grafts. Taken together, these bilayer grafts will be greatly promising candidates for SDVGs with rapid endothelialization and anti-calcification properties.http://www.sciencedirect.com/science/article/pii/S2452199X23002335Small-diameter vascular graftsNitric oxideHydrogen sulfideKeratinEndothelialization |
| spellingShingle | Pengfei Li Fubang Liang Lijuan Wang Dawei Jin Yushuang Shang Xu Liu Yanjun Pan Jiang Yuan Jian Shen Meng Yin Bilayer vascular grafts with on-demand NO and H2S release capabilities Bioactive Materials Small-diameter vascular grafts Nitric oxide Hydrogen sulfide Keratin Endothelialization |
| title | Bilayer vascular grafts with on-demand NO and H2S release capabilities |
| title_full | Bilayer vascular grafts with on-demand NO and H2S release capabilities |
| title_fullStr | Bilayer vascular grafts with on-demand NO and H2S release capabilities |
| title_full_unstemmed | Bilayer vascular grafts with on-demand NO and H2S release capabilities |
| title_short | Bilayer vascular grafts with on-demand NO and H2S release capabilities |
| title_sort | bilayer vascular grafts with on demand no and h2s release capabilities |
| topic | Small-diameter vascular grafts Nitric oxide Hydrogen sulfide Keratin Endothelialization |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X23002335 |
| work_keys_str_mv | AT pengfeili bilayervasculargraftswithondemandnoandh2sreleasecapabilities AT fubangliang bilayervasculargraftswithondemandnoandh2sreleasecapabilities AT lijuanwang bilayervasculargraftswithondemandnoandh2sreleasecapabilities AT daweijin bilayervasculargraftswithondemandnoandh2sreleasecapabilities AT yushuangshang bilayervasculargraftswithondemandnoandh2sreleasecapabilities AT xuliu bilayervasculargraftswithondemandnoandh2sreleasecapabilities AT yanjunpan bilayervasculargraftswithondemandnoandh2sreleasecapabilities AT jiangyuan bilayervasculargraftswithondemandnoandh2sreleasecapabilities AT jianshen bilayervasculargraftswithondemandnoandh2sreleasecapabilities AT mengyin bilayervasculargraftswithondemandnoandh2sreleasecapabilities |