Investigating the association between the tissue expression of miRNA‐101, JAK2/STAT3 with TNF‐α, IL‐6, IL‐1β, and IL‐10 cytokines in the ulcerative colitis patients
Abstract Background Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by numerous factors, such as immune system dysfunction and genetic factors. MicroRNAs (miRNAs) play a crucial role in UC pathogenesis, particularly via the JAK‐STAT pathway. Our aim was to investigate the asso...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-03-01
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| Series: | Immunity, Inflammation and Disease |
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| Online Access: | https://doi.org/10.1002/iid3.1224 |
| _version_ | 1797242527205556224 |
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| author | Qazaleh Voshagh Amir Anoshiravani Amin Karimpour Golnaz Goodarzi Sadra Samavarchi Tehrani Ozra Tabatabaei‐Malazy Ghodratollah Panahi |
| author_facet | Qazaleh Voshagh Amir Anoshiravani Amin Karimpour Golnaz Goodarzi Sadra Samavarchi Tehrani Ozra Tabatabaei‐Malazy Ghodratollah Panahi |
| author_sort | Qazaleh Voshagh |
| collection | DOAJ |
| description | Abstract Background Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by numerous factors, such as immune system dysfunction and genetic factors. MicroRNAs (miRNAs) play a crucial role in UC pathogenesis, particularly via the JAK‐STAT pathway. Our aim was to investigate the association between miRNA‐101 and JAK2‐STAT3 signaling pathway with inflammatory cytokines in UC patients. Methods We enrolled 35 UC patients and 35 healthy individuals as the control group, referred to Shariati Hospital, Tehran, Iran. Patients were diagnosed based on clinical, laboratory, histological, and colonoscopy criteria. RNA and protein extracted from tissue samples. Real‐time PCR was used to assess the expression levels of miRNA‐101, interleukin (IL)‐1β, IL‐6, tumor necrosis factor (TNF)‐α, and IL‐10 genes, while western blot was employed to measure levels of P‐STAT3, total STAT3, and JAK2 proteins. Results Expression of pro‐inflammatory cytokines TNF‐α, IL‐1β, and IL‐6 significantly increased, while the expression of IL‐10 significantly decreased in the case group versus controls. Additionally, miRNA‐101 expression was significantly higher in UC patients. A significant correlation between miRNA‐101 and IL‐6 expression was observed, indicating their relationship and possible impact on cell signaling pathways, JAK2‐STAT3. No significant changes were observed in phosphorylated and total STAT3 and JAK2 protein expression. Conclusion This study provides evidence of increased miRNA‐101 expression in UC tissue, suggesting a potential correlation between miRNA‐101 and IL‐6 expression and their involvement in the JAK2‐STAT3 pathway. The study confirms alterations in UC patients' pro‐inflammatory cytokines and anti‐inflammatory IL‐10. However, further investigations are needed to understand the exact role of miRNA‐101 in UC pathogenesis fully. |
| first_indexed | 2024-04-24T18:40:38Z |
| format | Article |
| id | doaj.art-1c9741eec43e4555b079fa065019e02b |
| institution | Directory Open Access Journal |
| issn | 2050-4527 |
| language | English |
| last_indexed | 2024-04-24T18:40:38Z |
| publishDate | 2024-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Immunity, Inflammation and Disease |
| spelling | doaj.art-1c9741eec43e4555b079fa065019e02b2024-03-27T12:16:38ZengWileyImmunity, Inflammation and Disease2050-45272024-03-01123n/an/a10.1002/iid3.1224Investigating the association between the tissue expression of miRNA‐101, JAK2/STAT3 with TNF‐α, IL‐6, IL‐1β, and IL‐10 cytokines in the ulcerative colitis patientsQazaleh Voshagh0Amir Anoshiravani1Amin Karimpour2Golnaz Goodarzi3Sadra Samavarchi Tehrani4Ozra Tabatabaei‐Malazy5Ghodratollah Panahi6Department of Clinical Biochemistry, School of Medicine Tehran University of Medical Sciences Tehran IranDigestive Disease Research Center, Digestive Disease Research Institute Tehran University of Medical Sciences Tehran IranDepartment of Clinical Biochemistry, School of Medicine Tehran University of Medical Sciences Tehran IranDepartment of Pathobiology and Laboratory Sciences, School of Medicine North Khorasan University of Medical Sciences Bojnurd IranEndocrine Research Center, Institute of Endocrinology and Metabolism Iran University of Medical Science Tehran IranNon‐Communicable Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute Tehran University of Medical Sciences Tehran IranDepartment of Clinical Biochemistry, School of Medicine Tehran University of Medical Sciences Tehran IranAbstract Background Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by numerous factors, such as immune system dysfunction and genetic factors. MicroRNAs (miRNAs) play a crucial role in UC pathogenesis, particularly via the JAK‐STAT pathway. Our aim was to investigate the association between miRNA‐101 and JAK2‐STAT3 signaling pathway with inflammatory cytokines in UC patients. Methods We enrolled 35 UC patients and 35 healthy individuals as the control group, referred to Shariati Hospital, Tehran, Iran. Patients were diagnosed based on clinical, laboratory, histological, and colonoscopy criteria. RNA and protein extracted from tissue samples. Real‐time PCR was used to assess the expression levels of miRNA‐101, interleukin (IL)‐1β, IL‐6, tumor necrosis factor (TNF)‐α, and IL‐10 genes, while western blot was employed to measure levels of P‐STAT3, total STAT3, and JAK2 proteins. Results Expression of pro‐inflammatory cytokines TNF‐α, IL‐1β, and IL‐6 significantly increased, while the expression of IL‐10 significantly decreased in the case group versus controls. Additionally, miRNA‐101 expression was significantly higher in UC patients. A significant correlation between miRNA‐101 and IL‐6 expression was observed, indicating their relationship and possible impact on cell signaling pathways, JAK2‐STAT3. No significant changes were observed in phosphorylated and total STAT3 and JAK2 protein expression. Conclusion This study provides evidence of increased miRNA‐101 expression in UC tissue, suggesting a potential correlation between miRNA‐101 and IL‐6 expression and their involvement in the JAK2‐STAT3 pathway. The study confirms alterations in UC patients' pro‐inflammatory cytokines and anti‐inflammatory IL‐10. However, further investigations are needed to understand the exact role of miRNA‐101 in UC pathogenesis fully.https://doi.org/10.1002/iid3.1224IBDinflammationJAK2‐STAT3miRNA‐101ulcerative colitis |
| spellingShingle | Qazaleh Voshagh Amir Anoshiravani Amin Karimpour Golnaz Goodarzi Sadra Samavarchi Tehrani Ozra Tabatabaei‐Malazy Ghodratollah Panahi Investigating the association between the tissue expression of miRNA‐101, JAK2/STAT3 with TNF‐α, IL‐6, IL‐1β, and IL‐10 cytokines in the ulcerative colitis patients Immunity, Inflammation and Disease IBD inflammation JAK2‐STAT3 miRNA‐101 ulcerative colitis |
| title | Investigating the association between the tissue expression of miRNA‐101, JAK2/STAT3 with TNF‐α, IL‐6, IL‐1β, and IL‐10 cytokines in the ulcerative colitis patients |
| title_full | Investigating the association between the tissue expression of miRNA‐101, JAK2/STAT3 with TNF‐α, IL‐6, IL‐1β, and IL‐10 cytokines in the ulcerative colitis patients |
| title_fullStr | Investigating the association between the tissue expression of miRNA‐101, JAK2/STAT3 with TNF‐α, IL‐6, IL‐1β, and IL‐10 cytokines in the ulcerative colitis patients |
| title_full_unstemmed | Investigating the association between the tissue expression of miRNA‐101, JAK2/STAT3 with TNF‐α, IL‐6, IL‐1β, and IL‐10 cytokines in the ulcerative colitis patients |
| title_short | Investigating the association between the tissue expression of miRNA‐101, JAK2/STAT3 with TNF‐α, IL‐6, IL‐1β, and IL‐10 cytokines in the ulcerative colitis patients |
| title_sort | investigating the association between the tissue expression of mirna 101 jak2 stat3 with tnf α il 6 il 1β and il 10 cytokines in the ulcerative colitis patients |
| topic | IBD inflammation JAK2‐STAT3 miRNA‐101 ulcerative colitis |
| url | https://doi.org/10.1002/iid3.1224 |
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