Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopy
Previous electron microscope studies of ultrastructural events during hepatitis A virus replication in experimentally infected cells have used only ultrathin section techniques. Nevertheless, no important differences were observed between infected and uninfected cells. This study was carried out usi...
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| Format: | Article |
| Language: | English |
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Vicerractoría Investigación
2002-06-01
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| Series: | Revista de Biología Tropical |
| Subjects: | |
| Online Access: | http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442002000200011 |
| _version_ | 1797714407601471488 |
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| author | Francisco Hernández-Chavarría Libertad Alzamora-González Libia Herrero-Uribe |
| author_facet | Francisco Hernández-Chavarría Libertad Alzamora-González Libia Herrero-Uribe |
| author_sort | Francisco Hernández-Chavarría |
| collection | DOAJ |
| description | Previous electron microscope studies of ultrastructural events during hepatitis A virus replication in experimentally infected cells have used only ultrathin section techniques. Nevertheless, no important differences were observed between infected and uninfected cells. This study was carried out using scanning electron microscopy and negative staining of whole LLC-MK2 cells grown directly on grids covered with support membranes, and then infected with an hepatitis A virus strain. Thin sections of infected and unifected controls were also analyzed. An intricate web of projections forming a net between cell interfaces was observed only in infected cells. Some of these projections were more than 700 nm long and had ballooning tips. Nevertheless, HAV particles were not visualized in the infected cells<br>Los estudios previos sobre los eventos ultraestructurales en la replicación del virus de la hepatitis Aen cultivos de células infectadas experimentalmente, se han realizado exclusivamente mediante cortes ultrafinos. No obstante, no se han observado diferencias importantes entre células infectadas y no infectadas. Este estudio fue realizado empleando microscopia electrónica de rastreo, tinción negativa de células (LLC-MK2) cultivadas directamente sobre rejillas para microscopia electrónica de transmisión e infectadas con el virus de la hepatitis A. Adicionalmente se analizaron cortes ultrafinos de células infectadas y no infectadas. Se observó una intrincada red de proyecciones entre las superficies de las células, que apareció solo en las células infectadas. Algunas de esas proyecciones medían más de 700 nm de largo y terminaban en extremos abultados. Sin embargo, no se observaron viriones en las células infectadas |
| first_indexed | 2024-03-12T07:51:26Z |
| format | Article |
| id | doaj.art-1c9820df56b44701a990975a8fdf67fd |
| institution | Directory Open Access Journal |
| issn | 0034-7744 2215-2075 |
| language | English |
| last_indexed | 2024-03-12T07:51:26Z |
| publishDate | 2002-06-01 |
| publisher | Vicerractoría Investigación |
| record_format | Article |
| series | Revista de Biología Tropical |
| spelling | doaj.art-1c9820df56b44701a990975a8fdf67fd2023-09-02T20:31:00ZengVicerractoría InvestigaciónRevista de Biología Tropical0034-77442215-20752002-06-01502519523Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopyFrancisco Hernández-ChavarríaLibertad Alzamora-GonzálezLibia Herrero-UribePrevious electron microscope studies of ultrastructural events during hepatitis A virus replication in experimentally infected cells have used only ultrathin section techniques. Nevertheless, no important differences were observed between infected and uninfected cells. This study was carried out using scanning electron microscopy and negative staining of whole LLC-MK2 cells grown directly on grids covered with support membranes, and then infected with an hepatitis A virus strain. Thin sections of infected and unifected controls were also analyzed. An intricate web of projections forming a net between cell interfaces was observed only in infected cells. Some of these projections were more than 700 nm long and had ballooning tips. Nevertheless, HAV particles were not visualized in the infected cells<br>Los estudios previos sobre los eventos ultraestructurales en la replicación del virus de la hepatitis Aen cultivos de células infectadas experimentalmente, se han realizado exclusivamente mediante cortes ultrafinos. No obstante, no se han observado diferencias importantes entre células infectadas y no infectadas. Este estudio fue realizado empleando microscopia electrónica de rastreo, tinción negativa de células (LLC-MK2) cultivadas directamente sobre rejillas para microscopia electrónica de transmisión e infectadas con el virus de la hepatitis A. Adicionalmente se analizaron cortes ultrafinos de células infectadas y no infectadas. Se observó una intrincada red de proyecciones entre las superficies de las células, que apareció solo en las células infectadas. Algunas de esas proyecciones medían más de 700 nm de largo y terminaban en extremos abultados. Sin embargo, no se observaron viriones en las células infectadashttp://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442002000200011Hepatitis A virusnegative stainingscanning electron microscopythin sectionsultrastructure |
| spellingShingle | Francisco Hernández-Chavarría Libertad Alzamora-González Libia Herrero-Uribe Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopy Revista de Biología Tropical Hepatitis A virus negative staining scanning electron microscopy thin sections ultrastructure |
| title | Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopy |
| title_full | Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopy |
| title_fullStr | Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopy |
| title_full_unstemmed | Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopy |
| title_short | Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopy |
| title_sort | noncytopathic hepatitis a virus induces surface alterations in llc mk2 cells revealed by thin sections negative staining and scanning electron microscopy |
| topic | Hepatitis A virus negative staining scanning electron microscopy thin sections ultrastructure |
| url | http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442002000200011 |
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