Comparison of Three Lateral Flow Immunoassay Formats for the Detection of Antibodies against the SARS-CoV-2 Antigen
Reliable detection of specific antibodies against pathogens by lateral flow immunoassay (LFIA) greatly depends on the composition of the detectable complex and the order of its assembly. We compared three LFIA formats for revealing anti-SARS-CoV-2 antibodies in sera with the following detected compl...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/7/750 |
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| author | Dmitriy V. Sotnikov Nadezhda A. Byzova Anatoly V. Zherdev Youchun Xu Boris B. Dzantiev |
| author_facet | Dmitriy V. Sotnikov Nadezhda A. Byzova Anatoly V. Zherdev Youchun Xu Boris B. Dzantiev |
| author_sort | Dmitriy V. Sotnikov |
| collection | DOAJ |
| description | Reliable detection of specific antibodies against pathogens by lateral flow immunoassay (LFIA) greatly depends on the composition of the detectable complex and the order of its assembly. We compared three LFIA formats for revealing anti-SARS-CoV-2 antibodies in sera with the following detected complexes in the analytical zone of the strip: antigen–antibodies–labeled immunoglobulin-binding protein (Scheme A); antigen–antibodies–labeled antigen (Scheme B); and immunoglobulin-binding protein–antibodies–labeled antigen (Scheme C). The lowest detection limit was observed for Scheme C, and was equal to 10 ng/mL of specific humanized monoclonal antibodies. When working with pooled positive sera, Scheme C had a detection limit 15 times lower than Scheme B and 255 times lower than Scheme A. Due to the high sensitivity of Scheme C, its application for the panel of human sera (n = 22) demonstrated 100% diagnostic specificity and sensitivity. These consistent results be useful for designing the format of LFIA serodiagnosis for other diseases. |
| first_indexed | 2024-03-11T01:14:18Z |
| format | Article |
| id | doaj.art-1c9b98e684194a02b5a8d9ce40c9b67d |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T01:14:18Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-1c9b98e684194a02b5a8d9ce40c9b67d2023-11-18T18:33:29ZengMDPI AGBiosensors2079-63742023-07-0113775010.3390/bios13070750Comparison of Three Lateral Flow Immunoassay Formats for the Detection of Antibodies against the SARS-CoV-2 AntigenDmitriy V. Sotnikov0Nadezhda A. Byzova1Anatoly V. Zherdev2Youchun Xu3Boris B. Dzantiev4A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaState Key Laboratory of Membrane Biology, Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, ChinaA.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaReliable detection of specific antibodies against pathogens by lateral flow immunoassay (LFIA) greatly depends on the composition of the detectable complex and the order of its assembly. We compared three LFIA formats for revealing anti-SARS-CoV-2 antibodies in sera with the following detected complexes in the analytical zone of the strip: antigen–antibodies–labeled immunoglobulin-binding protein (Scheme A); antigen–antibodies–labeled antigen (Scheme B); and immunoglobulin-binding protein–antibodies–labeled antigen (Scheme C). The lowest detection limit was observed for Scheme C, and was equal to 10 ng/mL of specific humanized monoclonal antibodies. When working with pooled positive sera, Scheme C had a detection limit 15 times lower than Scheme B and 255 times lower than Scheme A. Due to the high sensitivity of Scheme C, its application for the panel of human sera (n = 22) demonstrated 100% diagnostic specificity and sensitivity. These consistent results be useful for designing the format of LFIA serodiagnosis for other diseases.https://www.mdpi.com/2079-6374/13/7/750immunochromatographyCOVID-19serodiagnosticsimmune complexesserum testing |
| spellingShingle | Dmitriy V. Sotnikov Nadezhda A. Byzova Anatoly V. Zherdev Youchun Xu Boris B. Dzantiev Comparison of Three Lateral Flow Immunoassay Formats for the Detection of Antibodies against the SARS-CoV-2 Antigen Biosensors immunochromatography COVID-19 serodiagnostics immune complexes serum testing |
| title | Comparison of Three Lateral Flow Immunoassay Formats for the Detection of Antibodies against the SARS-CoV-2 Antigen |
| title_full | Comparison of Three Lateral Flow Immunoassay Formats for the Detection of Antibodies against the SARS-CoV-2 Antigen |
| title_fullStr | Comparison of Three Lateral Flow Immunoassay Formats for the Detection of Antibodies against the SARS-CoV-2 Antigen |
| title_full_unstemmed | Comparison of Three Lateral Flow Immunoassay Formats for the Detection of Antibodies against the SARS-CoV-2 Antigen |
| title_short | Comparison of Three Lateral Flow Immunoassay Formats for the Detection of Antibodies against the SARS-CoV-2 Antigen |
| title_sort | comparison of three lateral flow immunoassay formats for the detection of antibodies against the sars cov 2 antigen |
| topic | immunochromatography COVID-19 serodiagnostics immune complexes serum testing |
| url | https://www.mdpi.com/2079-6374/13/7/750 |
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