O6 Cardiac mitochondrial dysfunction time course in Type 1 Diabetes
Introduction: Results from our laboratory (Bombicino et al., 2016, 2017) have shown that sustained hyperglycemia for 25 days leads to generalized cardiac mitochondrial dysfunction that includes reduction of tissue and mitochondrial O2 consumption, complexes I-III, II-III and IV activities, ATP produ...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | Spanish |
| Published: |
Sello Editorial Lugones
2020-11-01
|
| Series: | Revista de la Sociedad Argentina de Diabetes |
| Subjects: | |
| Online Access: | https://revistasad.com/index.php/diabetes/article/view/367 |
| _version_ | 1811217058300428288 |
|---|---|
| author | Ivana Rukavina Mikusic Micaela Rey Manuela Martinefski Valeria Trípodi Laura Beatriz Valdez |
| author_facet | Ivana Rukavina Mikusic Micaela Rey Manuela Martinefski Valeria Trípodi Laura Beatriz Valdez |
| author_sort | Ivana Rukavina Mikusic |
| collection | DOAJ |
| description | Introduction: Results from our laboratory (Bombicino et al., 2016, 2017) have shown that sustained hyperglycemia for 25 days leads to generalized cardiac mitochondrial dysfunction that includes reduction of tissue and mitochondrial O2 consumption, complexes I-III, II-III and IV activities, ATP production, ADP/O, and Mn-SOD activity; accompanied by enhancement of H2O2, NO and ONOO- generation rates, in addition to the triggering of mitochondrial biogenesis, although the "new" cardiac mitochondria of diabetic animals show structural alterations. This mitochondrial dysfunction occurs in the absence of hypertrophy and of changes in resting cardiac performance, but with cardiac compromise after a work overload, suggesting that mitochondrial dysfunction precedes myocardial failure in diabetic patients.
Aim: To study the early events and to analyze the cardiac mitochondrial dysfunction time course in a type 1 Diabetes Mellitus model.
Materials and methods: Diabetes was induced by Streptozotocin (STZ, single ip dose, 60 mg/kg) in male Wistar rats. Glycemia was measured at 72 h (C: 127 ± 5, DM: 415 ± 23 mg/dl). Animals were sacrificed 7, 10 or 14 days after STZ-injection (4, 7 or 11 days of hyperglycemia) and hearts were removed. Mitochondrial function and biogenesis, reactive oxygen and nitrogen species generation and redox state were determined |
| first_indexed | 2024-04-12T06:47:48Z |
| format | Article |
| id | doaj.art-1c9dff77db694da9a8739bb2edbe79ff |
| institution | Directory Open Access Journal |
| issn | 0325-5247 2346-9420 |
| language | Spanish |
| last_indexed | 2024-04-12T06:47:48Z |
| publishDate | 2020-11-01 |
| publisher | Sello Editorial Lugones |
| record_format | Article |
| series | Revista de la Sociedad Argentina de Diabetes |
| spelling | doaj.art-1c9dff77db694da9a8739bb2edbe79ff2022-12-22T03:43:27ZspaSello Editorial LugonesRevista de la Sociedad Argentina de Diabetes0325-52472346-94202020-11-01543Sup919110.47196/diab.v54i3Sup.367301O6 Cardiac mitochondrial dysfunction time course in Type 1 DiabetesIvana Rukavina Mikusic0Micaela Rey1Manuela Martinefski2Valeria Trípodi3Laura Beatriz Valdez4UNIVERSIDAD DE BUENOS AIRES, FACULTAD DE FARMACIA Y BIOQUÍMICA, FISICOQUÍMICA,INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR (IBIMOL), UNIVERSIDAD DE BUENOS AIRES, CONSEJO NACIONAL DE INVESTIGACIONES CIENTÍFICAS Y TÉCNICAS (UBA-CONICET), CIUDAD AUTÓNOMA DE BUENOS AIRES, ARGENTINAUNIVERSIDAD DE BUENOS AIRES, FACULTAD DE FARMACIA Y BIOQUÍMICA, FISICOQUÍMICA,CIUDAD AUTÓNOMA DE BUENOS AIRES, ARGENTINAUNIVERSIDAD DE BUENOS AIRES, FACULTAD DE FARMACIA Y BIOQUÍMICA, TECNOLOGÍA FARMACÉUTICA,CIUDAD AUTÓNOMA DE BUENOS AIRES, ARGENTINAUNIVERSIDAD DE BUENOS AIRES, FACULTAD DE FARMACIA Y BIOQUÍMICA, TECNOLOGÍA FARMACÉUTICA,CIUDAD AUTÓNOMA DE BUENOS AIRES, ARGENTINAUNIVERSIDAD DE BUENOS AIRES, FACULTAD DE FARMACIA Y BIOQUÍMICA, FISICOQUÍMICA,INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR (IBIMOL), UNIVERSIDAD DE BUENOS AIRES, CONSEJO NACIONAL DE INVESTIGACIONES CIENTÍFICAS Y TÉCNICAS (UBA-CONICET), CIUDAD AUTÓNOMA DE BUENOS AIRES, ARGENTINAIntroduction: Results from our laboratory (Bombicino et al., 2016, 2017) have shown that sustained hyperglycemia for 25 days leads to generalized cardiac mitochondrial dysfunction that includes reduction of tissue and mitochondrial O2 consumption, complexes I-III, II-III and IV activities, ATP production, ADP/O, and Mn-SOD activity; accompanied by enhancement of H2O2, NO and ONOO- generation rates, in addition to the triggering of mitochondrial biogenesis, although the "new" cardiac mitochondria of diabetic animals show structural alterations. This mitochondrial dysfunction occurs in the absence of hypertrophy and of changes in resting cardiac performance, but with cardiac compromise after a work overload, suggesting that mitochondrial dysfunction precedes myocardial failure in diabetic patients. Aim: To study the early events and to analyze the cardiac mitochondrial dysfunction time course in a type 1 Diabetes Mellitus model. Materials and methods: Diabetes was induced by Streptozotocin (STZ, single ip dose, 60 mg/kg) in male Wistar rats. Glycemia was measured at 72 h (C: 127 ± 5, DM: 415 ± 23 mg/dl). Animals were sacrificed 7, 10 or 14 days after STZ-injection (4, 7 or 11 days of hyperglycemia) and hearts were removed. Mitochondrial function and biogenesis, reactive oxygen and nitrogen species generation and redox state were determinedhttps://revistasad.com/index.php/diabetes/article/view/367disfunción mitocondrial cardíacaevolucióndiabetes tipo 1 |
| spellingShingle | Ivana Rukavina Mikusic Micaela Rey Manuela Martinefski Valeria Trípodi Laura Beatriz Valdez O6 Cardiac mitochondrial dysfunction time course in Type 1 Diabetes Revista de la Sociedad Argentina de Diabetes disfunción mitocondrial cardíaca evolución diabetes tipo 1 |
| title | O6 Cardiac mitochondrial dysfunction time course in Type 1 Diabetes |
| title_full | O6 Cardiac mitochondrial dysfunction time course in Type 1 Diabetes |
| title_fullStr | O6 Cardiac mitochondrial dysfunction time course in Type 1 Diabetes |
| title_full_unstemmed | O6 Cardiac mitochondrial dysfunction time course in Type 1 Diabetes |
| title_short | O6 Cardiac mitochondrial dysfunction time course in Type 1 Diabetes |
| title_sort | o6 cardiac mitochondrial dysfunction time course in type 1 diabetes |
| topic | disfunción mitocondrial cardíaca evolución diabetes tipo 1 |
| url | https://revistasad.com/index.php/diabetes/article/view/367 |
| work_keys_str_mv | AT ivanarukavinamikusic o6cardiacmitochondrialdysfunctiontimecourseintype1diabetes AT micaelarey o6cardiacmitochondrialdysfunctiontimecourseintype1diabetes AT manuelamartinefski o6cardiacmitochondrialdysfunctiontimecourseintype1diabetes AT valeriatripodi o6cardiacmitochondrialdysfunctiontimecourseintype1diabetes AT laurabeatrizvaldez o6cardiacmitochondrialdysfunctiontimecourseintype1diabetes |