Long-Term Survey Is Necessary to Reveal Various Shifts of Microbial Composition in Corals
The coral holobiont is the assemblage of coral host and its microbial symbionts, which functions as a unit and is responsive to host species and environmental factors. Although monitoring surveys have been done to determine bacteria associated with coral, none have persisted for >1 year. Ther...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01094/full |
| _version_ | 1818036641253556224 |
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| author | Shan-Hua Yang Ching-Hung Tseng Chang-Rung Huang Chung-Pin Chen Kshitij Tandon Kshitij Tandon Kshitij Tandon Sonny T. M. Lee Pei-Wen Chiang Jia-Ho Shiu Jia-Ho Shiu Jia-Ho Shiu Chaolun A. Chen Sen-Lin Tang |
| author_facet | Shan-Hua Yang Ching-Hung Tseng Chang-Rung Huang Chung-Pin Chen Kshitij Tandon Kshitij Tandon Kshitij Tandon Sonny T. M. Lee Pei-Wen Chiang Jia-Ho Shiu Jia-Ho Shiu Jia-Ho Shiu Chaolun A. Chen Sen-Lin Tang |
| author_sort | Shan-Hua Yang |
| collection | DOAJ |
| description | The coral holobiont is the assemblage of coral host and its microbial symbionts, which functions as a unit and is responsive to host species and environmental factors. Although monitoring surveys have been done to determine bacteria associated with coral, none have persisted for >1 year. Therefore, potential variations in minor or dominant community members that occur over extended intervals have not been characterized. In this study, 16S rRNA gene amplicon pyrosequencing was used to investigate the relationship between bacterial communities in healthy Stylophora pistillata in tropical and subtropical Taiwan over 2 years, apparently one of the longest surveys of coral-associated microbes. Dominant bacterial genera in S. pistillata had disparate changes in different geographical setups, whereas the constitution of minor bacteria fluctuated in abundance over time. We concluded that dominant bacteria (Acinetobacter, Propionibacterium, and Pseudomonas) were stable in composition, regardless of seasonal and geographical variations, whereas Endozoicomonas had a geographical preference. In addition, by combining current data with previous studies, we concluded that a minor bacteria symbiont, Ralstonia, was a keystone species in coral. Finally, we concluded that long-term surveys for coral microbial communities were necessary to detect compositional shifts, especially for minor bacterial members in corals. |
| first_indexed | 2024-12-10T07:14:11Z |
| format | Article |
| id | doaj.art-1c9ecc0da9594a81a1e445b71369a47f |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T07:14:11Z |
| publishDate | 2017-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-1c9ecc0da9594a81a1e445b71369a47f2022-12-22T01:57:58ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-06-01810.3389/fmicb.2017.01094265131Long-Term Survey Is Necessary to Reveal Various Shifts of Microbial Composition in CoralsShan-Hua Yang0Ching-Hung Tseng1Chang-Rung Huang2Chung-Pin Chen3Kshitij Tandon4Kshitij Tandon5Kshitij Tandon6Sonny T. M. Lee7Pei-Wen Chiang8Jia-Ho Shiu9Jia-Ho Shiu10Jia-Ho Shiu11Chaolun A. Chen12Sen-Lin Tang13Biodiversity Research Center, Academia SinicaTaipei, TaiwanGermark Biotechnology Co., Ltd.Taichung, TaiwanBiodiversity Research Center, Academia SinicaTaipei, TaiwanYourgene BioscienceNew Taipei City, TaiwanBiodiversity Research Center, Academia SinicaTaipei, TaiwanBioinformatics Program, Institute of Information Science, Taiwan International Graduate Program, Academia SinicaTaipei, TaiwanInstitute of Bioinformatics and Structural Biology, National Tsing Hua UniversityHsinchu, TaiwanSection of Gastroenterology, Hepatology and Nutrition, Department of Medicine, University of Chicago Medicine, ChicagoIL, United StatesBiodiversity Research Center, Academia SinicaTaipei, TaiwanBiodiversity Research Center, Academia SinicaTaipei, TaiwanMolecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia SinicaTaipei, TaiwanGraduate Institute of Biotechnology, National Chung Hsing UniversityTaichung, TaiwanBiodiversity Research Center, Academia SinicaTaipei, TaiwanBiodiversity Research Center, Academia SinicaTaipei, TaiwanThe coral holobiont is the assemblage of coral host and its microbial symbionts, which functions as a unit and is responsive to host species and environmental factors. Although monitoring surveys have been done to determine bacteria associated with coral, none have persisted for >1 year. Therefore, potential variations in minor or dominant community members that occur over extended intervals have not been characterized. In this study, 16S rRNA gene amplicon pyrosequencing was used to investigate the relationship between bacterial communities in healthy Stylophora pistillata in tropical and subtropical Taiwan over 2 years, apparently one of the longest surveys of coral-associated microbes. Dominant bacterial genera in S. pistillata had disparate changes in different geographical setups, whereas the constitution of minor bacteria fluctuated in abundance over time. We concluded that dominant bacteria (Acinetobacter, Propionibacterium, and Pseudomonas) were stable in composition, regardless of seasonal and geographical variations, whereas Endozoicomonas had a geographical preference. In addition, by combining current data with previous studies, we concluded that a minor bacteria symbiont, Ralstonia, was a keystone species in coral. Finally, we concluded that long-term surveys for coral microbial communities were necessary to detect compositional shifts, especially for minor bacterial members in corals.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01094/fulllong-term surveymicrobial compositioncoralsStylophora pistillatashifts |
| spellingShingle | Shan-Hua Yang Ching-Hung Tseng Chang-Rung Huang Chung-Pin Chen Kshitij Tandon Kshitij Tandon Kshitij Tandon Sonny T. M. Lee Pei-Wen Chiang Jia-Ho Shiu Jia-Ho Shiu Jia-Ho Shiu Chaolun A. Chen Sen-Lin Tang Long-Term Survey Is Necessary to Reveal Various Shifts of Microbial Composition in Corals Frontiers in Microbiology long-term survey microbial composition corals Stylophora pistillata shifts |
| title | Long-Term Survey Is Necessary to Reveal Various Shifts of Microbial Composition in Corals |
| title_full | Long-Term Survey Is Necessary to Reveal Various Shifts of Microbial Composition in Corals |
| title_fullStr | Long-Term Survey Is Necessary to Reveal Various Shifts of Microbial Composition in Corals |
| title_full_unstemmed | Long-Term Survey Is Necessary to Reveal Various Shifts of Microbial Composition in Corals |
| title_short | Long-Term Survey Is Necessary to Reveal Various Shifts of Microbial Composition in Corals |
| title_sort | long term survey is necessary to reveal various shifts of microbial composition in corals |
| topic | long-term survey microbial composition corals Stylophora pistillata shifts |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01094/full |
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