Molecular cloning, expression and adhesion analysis of silent slpB of Lactobacillus acidophilus NCFM

Molecular cloning, expression and adhesion analysis of silent slpB of Lactobacillus acidophilus NCFM

Abstract The slpB gene of Lactobacillus acidophilus NCFM, which differs from the slpA gene and is silent under normal conditions, was successfully amplified and ligated to the corresponding available sites on a recombinant pET-28a vector. Then the pET-28a-slpB vector was transformed into Escherichia...

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Bibliographic Details
Main Authors: Yuxing Guo, Xiangyue Li, Yao Yang, Zhen Wu, Xiaoqun Zeng, Fawze Nadari, Daodong Pan
Format: Article
Language:English
Published: SpringerOpen 2018-06-01
Series:AMB Express
Subjects:
Adhesion
Lactobacillus acidophilus NCFM
S-layer protein
Caco-2 cell
pET-28a-slpB
His-slpB fusion
Online Access:http://link.springer.com/article/10.1186/s13568-018-0631-2

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