Protocol to analyze and quantify protein-methylated RNA interactions in mammalian cells with a combination of RNA immunoprecipitation and nucleoside mass spectrometry

Protocol to analyze and quantify protein-methylated RNA interactions in mammalian cells with a combination of RNA immunoprecipitation and nucleoside mass spectrometry

Summary: Cellular RNAs are modified by both physiological factors and exogenous agents, such as methyl methanesulfonate (MMS). However, techniques for analyzing how proteins may interact with these modified RNAs are limited. Here, we provide a protocol combining RNA immunoprecipitation (RIP) with ma...

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Bibliographic Details
Main Authors: Ning Tsao, Jennifer M. Soll, Nima Mosammaparast
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Molecular Biology
Protein Biochemistry
Proteomics
Mass Spectrometry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722001484

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