Ultrasound-mediated gene delivery into suspended plant cells using polyethyleneimine-coated mesoporous silica nanoparticles
Sonoporation, ultrasound-mediated membrane perforation can potentially puncture plasma membrane and rigid cell wall on presumably reversible basis which benefit gene transfection and plant biotechnology. Herein, positively charged poly-ethyleneimine (PEI)-coated mesoporous silica nanoparticles (MSNs...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-05-01
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| Series: | Ultrasonics Sonochemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417721000493 |
| _version_ | 1818955851067555840 |
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| author | Maryam Zolghadrnasab Amir Mousavi Abbas Farmany Ayyoob Arpanaei |
| author_facet | Maryam Zolghadrnasab Amir Mousavi Abbas Farmany Ayyoob Arpanaei |
| author_sort | Maryam Zolghadrnasab |
| collection | DOAJ |
| description | Sonoporation, ultrasound-mediated membrane perforation can potentially puncture plasma membrane and rigid cell wall on presumably reversible basis which benefit gene transfection and plant biotechnology. Herein, positively charged poly-ethyleneimine (PEI)-coated mesoporous silica nanoparticles (MSNs) with an average diameter of 100 ± 8.7 nm was synthesized for GUS-encoding plasmid delivery into the suspended tobacco cells using the ultrasound treatment. The overall potential of PEI-MSN for DNA adsorption was measured at 43.43 μg DNA mg−1 PEI-MSNs. It was shown that high level of sonoporation may adversely upset the cell viability. Optimal conditions of ultrasonic treatment are obtained as 8 min at 3 various intensities of 160, 320 and 640 W. Histochemical staining assay was used to follow the protein expression. It was shown that PEI-coated MSNs efficiently transfer the GUS-encoding plasmid DNA into the tobacco cells. The results of this study showed that ultrasonic treatment provides an economical and straightforward approach for gene transferring into the plant cells without any need to complicated devices and concerns about safety issues. |
| first_indexed | 2024-12-20T10:44:37Z |
| format | Article |
| id | doaj.art-1ca3dc77f951439088e476311b853a7d |
| institution | Directory Open Access Journal |
| issn | 1350-4177 |
| language | English |
| last_indexed | 2024-12-20T10:44:37Z |
| publishDate | 2021-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj.art-1ca3dc77f951439088e476311b853a7d2022-12-21T19:43:26ZengElsevierUltrasonics Sonochemistry1350-41772021-05-0173105507Ultrasound-mediated gene delivery into suspended plant cells using polyethyleneimine-coated mesoporous silica nanoparticlesMaryam Zolghadrnasab0Amir Mousavi1Abbas Farmany2Ayyoob Arpanaei3Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P. O. BOX 1417863171, Tehran, IranDepartment of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P. O. BOX 1417863171, Tehran, IranDental Research Center, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, IranDepartment of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P. O. BOX 1417863171, Tehran, Iran; Corresponding author.Sonoporation, ultrasound-mediated membrane perforation can potentially puncture plasma membrane and rigid cell wall on presumably reversible basis which benefit gene transfection and plant biotechnology. Herein, positively charged poly-ethyleneimine (PEI)-coated mesoporous silica nanoparticles (MSNs) with an average diameter of 100 ± 8.7 nm was synthesized for GUS-encoding plasmid delivery into the suspended tobacco cells using the ultrasound treatment. The overall potential of PEI-MSN for DNA adsorption was measured at 43.43 μg DNA mg−1 PEI-MSNs. It was shown that high level of sonoporation may adversely upset the cell viability. Optimal conditions of ultrasonic treatment are obtained as 8 min at 3 various intensities of 160, 320 and 640 W. Histochemical staining assay was used to follow the protein expression. It was shown that PEI-coated MSNs efficiently transfer the GUS-encoding plasmid DNA into the tobacco cells. The results of this study showed that ultrasonic treatment provides an economical and straightforward approach for gene transferring into the plant cells without any need to complicated devices and concerns about safety issues.http://www.sciencedirect.com/science/article/pii/S1350417721000493Ultrasonic treatmentGene transfectionGUS-encoding plasmid DNAMesoporous silica nanoparticles |
| spellingShingle | Maryam Zolghadrnasab Amir Mousavi Abbas Farmany Ayyoob Arpanaei Ultrasound-mediated gene delivery into suspended plant cells using polyethyleneimine-coated mesoporous silica nanoparticles Ultrasonics Sonochemistry Ultrasonic treatment Gene transfection GUS-encoding plasmid DNA Mesoporous silica nanoparticles |
| title | Ultrasound-mediated gene delivery into suspended plant cells using polyethyleneimine-coated mesoporous silica nanoparticles |
| title_full | Ultrasound-mediated gene delivery into suspended plant cells using polyethyleneimine-coated mesoporous silica nanoparticles |
| title_fullStr | Ultrasound-mediated gene delivery into suspended plant cells using polyethyleneimine-coated mesoporous silica nanoparticles |
| title_full_unstemmed | Ultrasound-mediated gene delivery into suspended plant cells using polyethyleneimine-coated mesoporous silica nanoparticles |
| title_short | Ultrasound-mediated gene delivery into suspended plant cells using polyethyleneimine-coated mesoporous silica nanoparticles |
| title_sort | ultrasound mediated gene delivery into suspended plant cells using polyethyleneimine coated mesoporous silica nanoparticles |
| topic | Ultrasonic treatment Gene transfection GUS-encoding plasmid DNA Mesoporous silica nanoparticles |
| url | http://www.sciencedirect.com/science/article/pii/S1350417721000493 |
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