Plasmodium yoelii: identification of a gene encoding a putative ADP-ribosylation factor-1 GTPase-activating Protein, PyAG1
The PyAG1 gene, identified by the screening of a Plasmodium yoelii genomic DNA library with a rhoptry-specific Mab, encodes a protein with a zinc finger structure immediately followed by the consensus sequence of the Arf GAP catalytic site. The serum of mice immunized with the recombinant protein re...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2000-06-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000300009 |
| _version_ | 1827879324134408192 |
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| author | Rémi Hienne Alain Rico Daniel Parzy Jean-Claude Doury |
| author_facet | Rémi Hienne Alain Rico Daniel Parzy Jean-Claude Doury |
| author_sort | Rémi Hienne |
| collection | DOAJ |
| description | The PyAG1 gene, identified by the screening of a Plasmodium yoelii genomic DNA library with a rhoptry-specific Mab, encodes a protein with a zinc finger structure immediately followed by the consensus sequence of the Arf GAP catalytic site. The serum of mice immunized with the recombinant protein recognized specifically the rhoptries of the late infected erythrocytic stages. Blast analysis using the Genbank database gave the highest scores with four proteins presenting an Arf1 GAP activity. If presenting also this activity, the PyAG1 protein could be involved in the regulation of the secreted protein vesicular transport and, consequently, in the rhoptry biogenesis. |
| first_indexed | 2024-03-12T18:05:08Z |
| format | Article |
| id | doaj.art-1ca6c83bb4d745b2aabb43c1be47c77e |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:05:08Z |
| publishDate | 2000-06-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-1ca6c83bb4d745b2aabb43c1be47c77e2023-08-02T09:29:06ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602000-06-0195334535210.1590/S0074-02762000000300009Plasmodium yoelii: identification of a gene encoding a putative ADP-ribosylation factor-1 GTPase-activating Protein, PyAG1Rémi HienneAlain RicoDaniel ParzyJean-Claude DouryThe PyAG1 gene, identified by the screening of a Plasmodium yoelii genomic DNA library with a rhoptry-specific Mab, encodes a protein with a zinc finger structure immediately followed by the consensus sequence of the Arf GAP catalytic site. The serum of mice immunized with the recombinant protein recognized specifically the rhoptries of the late infected erythrocytic stages. Blast analysis using the Genbank database gave the highest scores with four proteins presenting an Arf1 GAP activity. If presenting also this activity, the PyAG1 protein could be involved in the regulation of the secreted protein vesicular transport and, consequently, in the rhoptry biogenesis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000300009Plasmodium yoeliigene PyAG1immature rhoptriesvesicular transport |
| spellingShingle | Rémi Hienne Alain Rico Daniel Parzy Jean-Claude Doury Plasmodium yoelii: identification of a gene encoding a putative ADP-ribosylation factor-1 GTPase-activating Protein, PyAG1 Memorias do Instituto Oswaldo Cruz Plasmodium yoelii gene PyAG1 immature rhoptries vesicular transport |
| title | Plasmodium yoelii: identification of a gene encoding a putative ADP-ribosylation factor-1 GTPase-activating Protein, PyAG1 |
| title_full | Plasmodium yoelii: identification of a gene encoding a putative ADP-ribosylation factor-1 GTPase-activating Protein, PyAG1 |
| title_fullStr | Plasmodium yoelii: identification of a gene encoding a putative ADP-ribosylation factor-1 GTPase-activating Protein, PyAG1 |
| title_full_unstemmed | Plasmodium yoelii: identification of a gene encoding a putative ADP-ribosylation factor-1 GTPase-activating Protein, PyAG1 |
| title_short | Plasmodium yoelii: identification of a gene encoding a putative ADP-ribosylation factor-1 GTPase-activating Protein, PyAG1 |
| title_sort | plasmodium yoelii identification of a gene encoding a putative adp ribosylation factor 1 gtpase activating protein pyag1 |
| topic | Plasmodium yoelii gene PyAG1 immature rhoptries vesicular transport |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000300009 |
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