Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi
Abstract Borrelia miyamotoi, a member of the tick-borne relapsing fever spirochetes, shows a serum-resistant phenotype in vitro. This ability of B. miyamotoi may contribute to bacterial evasion of the host innate immune system. To investigate the molecular mechanism of serum-resistance, we construct...
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Nature Portfolio
2021-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-85069-w |
| _version_ | 1818425634056044544 |
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| author | Kozue Sato Yumi Kumagai Tsuyoshi Sekizuka Makoto Kuroda Tetsuya Hayashi Ai Takano Gaowa Kyle R. Taylor Makoto Ohnishi Hiroki Kawabata |
| author_facet | Kozue Sato Yumi Kumagai Tsuyoshi Sekizuka Makoto Kuroda Tetsuya Hayashi Ai Takano Gaowa Kyle R. Taylor Makoto Ohnishi Hiroki Kawabata |
| author_sort | Kozue Sato |
| collection | DOAJ |
| description | Abstract Borrelia miyamotoi, a member of the tick-borne relapsing fever spirochetes, shows a serum-resistant phenotype in vitro. This ability of B. miyamotoi may contribute to bacterial evasion of the host innate immune system. To investigate the molecular mechanism of serum-resistance, we constructed a membrane protein-encoding gene library of B. miyamotoi using Borrelia garinii strain HT59G, which shows a transformable and serum-susceptible phenotype. By screening the library, we found that bom1093 and bom1515 of B. miyamotoi provided a serum-resistant phenotype to the recipient B. garinii. These B. miyamotoi genes are predicted to encode P35-like antigen genes and are conserved among relapsing fever borreliae. Functional analysis revealed that BOM1093 bound to serum vitronectin and that the C-terminal region of BOM1093 was involved in the vitronectin-binding property. Importantly, the B. garinii transformant was not serum-resistant when the C terminus-truncated BOM1093 was expressed. We also observed that the depletion of vitronectin from human serum enhances the bactericidal activity of BOM1093 expressing B. garinii, and the survival rate of BOM1093 expressing B. garinii in vitronectin-depleted serum is enhanced by the addition of purified vitronectin. Our data suggests that B. miyamotoi utilize BOM1093-mediated binding to vitronectin as a mechanism of serum resistance. |
| first_indexed | 2024-12-14T14:17:03Z |
| format | Article |
| id | doaj.art-1ca93b4dd78d42f4a47f6c2bc832432d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T14:17:03Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1ca93b4dd78d42f4a47f6c2bc832432d2022-12-21T22:58:11ZengNature PortfolioScientific Reports2045-23222021-03-0111111310.1038/s41598-021-85069-wVitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoiKozue Sato0Yumi Kumagai1Tsuyoshi Sekizuka2Makoto Kuroda3Tetsuya Hayashi4Ai Takano5Gaowa6Kyle R. Taylor7Makoto Ohnishi8Hiroki Kawabata9Department of Bacteriology-I, National Institute of Infectious DiseaseDepartment of Bacteriology-I, National Institute of Infectious DiseasePathogen Genomics Center, National Institute of Infectious DiseasePathogen Genomics Center, National Institute of Infectious DiseaseDepartment of Bacteriology, Faculty of Medical Sciences, Kyushu UniversityLaboratory of Veterinary Epidemiology, Joint Faculty of Veterinary Medicine, Yamaguchi UniversityInner Mongolia Key Laboratory of Tick-Borne Zoonotic Infectious Disease, Department of Medicine, College of HetaoCollege of Veterinary Medicine, Washington State UniversityDepartment of Bacteriology-I, National Institute of Infectious DiseaseDepartment of Bacteriology-I, National Institute of Infectious DiseaseAbstract Borrelia miyamotoi, a member of the tick-borne relapsing fever spirochetes, shows a serum-resistant phenotype in vitro. This ability of B. miyamotoi may contribute to bacterial evasion of the host innate immune system. To investigate the molecular mechanism of serum-resistance, we constructed a membrane protein-encoding gene library of B. miyamotoi using Borrelia garinii strain HT59G, which shows a transformable and serum-susceptible phenotype. By screening the library, we found that bom1093 and bom1515 of B. miyamotoi provided a serum-resistant phenotype to the recipient B. garinii. These B. miyamotoi genes are predicted to encode P35-like antigen genes and are conserved among relapsing fever borreliae. Functional analysis revealed that BOM1093 bound to serum vitronectin and that the C-terminal region of BOM1093 was involved in the vitronectin-binding property. Importantly, the B. garinii transformant was not serum-resistant when the C terminus-truncated BOM1093 was expressed. We also observed that the depletion of vitronectin from human serum enhances the bactericidal activity of BOM1093 expressing B. garinii, and the survival rate of BOM1093 expressing B. garinii in vitronectin-depleted serum is enhanced by the addition of purified vitronectin. Our data suggests that B. miyamotoi utilize BOM1093-mediated binding to vitronectin as a mechanism of serum resistance.https://doi.org/10.1038/s41598-021-85069-w |
| spellingShingle | Kozue Sato Yumi Kumagai Tsuyoshi Sekizuka Makoto Kuroda Tetsuya Hayashi Ai Takano Gaowa Kyle R. Taylor Makoto Ohnishi Hiroki Kawabata Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi Scientific Reports |
| title | Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi |
| title_full | Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi |
| title_fullStr | Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi |
| title_full_unstemmed | Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi |
| title_short | Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi |
| title_sort | vitronectin binding protein bom1093 confers serum resistance on borrelia miyamotoi |
| url | https://doi.org/10.1038/s41598-021-85069-w |
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