A Polysaccharide Isolated from the Herb <i>Bletilla striata</i> Combined with Methylcellulose to Form a Hydrogel via Self-Assembly as a Wound Dressing
The <i>Bletilla striata</i> Polysaccharide (BSP), a natural polysaccharide derived from the east Asian terrestrial orchid <i>Bletilla striata</i>, is an anti-inflammatory, antiviral, and antioxidant polysaccharide. Traditionally, it has been used to treat hemostasis and for w...
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| author | Subhaini Jakfar Tzu-Chieh Lin Zhi-Yu Chen I-Hsuan Yang Basri A. Gani Diana Setya Ningsih Hendra Kusuma Chia-Tien Chang Feng-Huei Lin |
| author_facet | Subhaini Jakfar Tzu-Chieh Lin Zhi-Yu Chen I-Hsuan Yang Basri A. Gani Diana Setya Ningsih Hendra Kusuma Chia-Tien Chang Feng-Huei Lin |
| author_sort | Subhaini Jakfar |
| collection | DOAJ |
| description | The <i>Bletilla striata</i> Polysaccharide (BSP), a natural polysaccharide derived from the east Asian terrestrial orchid <i>Bletilla striata</i>, is an anti-inflammatory, antiviral, and antioxidant polysaccharide. Traditionally, it has been used to treat hemostasis and for wound healing. In this study, BSP was blended with methylcellulose (MC) and methylparaben (MP) to create a hydrogel through a self-assembly route as a wound dressing. The developed hydrogels were designed as M2B<i>x</i>, M5B<i>x</i>, and M8B<i>x</i>. M stands for MC, and the number represents a percentage. Whereas the second letter of B stands for BSP, and <i>x</i> refers to the percentage variation of BSP: <i>x</i> = 0.5%, 1%, and 2%. All the developed MB hydrogels contained β-glucopyranosyl and α-mannopyranosyl, and rheology test had a tan δ value ≥ 0.5. The pore sizes of the hydrogels decreased by increasing the MC and BSP content, and they had better properties with respect to water loss and their swelling ratio. Evaluations in vitro and in vivo showed that all of the developed MB hydrogels have good cell viability and wound-healing properties. The M8B2 hydrogel group was found to be superior to the others from within the developed MB hydrogels. Therefore, we believe that the M8B2 hydrogel formulation has a high potential for development as a wound dressing. |
| first_indexed | 2024-03-09T21:36:00Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:36:00Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1cad7e193b75491fa67cef1ea166ebaa2023-11-23T20:43:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123191201910.3390/ijms231912019A Polysaccharide Isolated from the Herb <i>Bletilla striata</i> Combined with Methylcellulose to Form a Hydrogel via Self-Assembly as a Wound DressingSubhaini Jakfar0Tzu-Chieh Lin1Zhi-Yu Chen2I-Hsuan Yang3Basri A. Gani4Diana Setya Ningsih5Hendra Kusuma6Chia-Tien Chang7Feng-Huei Lin8Institute of Biomedical Engineering, National Taiwan University, Taipei 106, TaiwanInstitute of Biomedical Engineering, National Taiwan University, Taipei 106, TaiwanInstitute of Biomedical Engineering, National Taiwan University, Taipei 106, TaiwanInstitute of Biomedical Engineering, National Taiwan University, Taipei 106, TaiwanDentistry Faculty, Syiah Kuala University, Darussalam, Banda Aceh 23111, IndonesiaDentistry Faculty, Syiah Kuala University, Darussalam, Banda Aceh 23111, IndonesiaAgriculture Faculty, Gajah Putih University, Aceh Tengah 24552, IndonesiaInstitute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Miaoli 350, TaiwanInstitute of Biomedical Engineering, National Taiwan University, Taipei 106, TaiwanThe <i>Bletilla striata</i> Polysaccharide (BSP), a natural polysaccharide derived from the east Asian terrestrial orchid <i>Bletilla striata</i>, is an anti-inflammatory, antiviral, and antioxidant polysaccharide. Traditionally, it has been used to treat hemostasis and for wound healing. In this study, BSP was blended with methylcellulose (MC) and methylparaben (MP) to create a hydrogel through a self-assembly route as a wound dressing. The developed hydrogels were designed as M2B<i>x</i>, M5B<i>x</i>, and M8B<i>x</i>. M stands for MC, and the number represents a percentage. Whereas the second letter of B stands for BSP, and <i>x</i> refers to the percentage variation of BSP: <i>x</i> = 0.5%, 1%, and 2%. All the developed MB hydrogels contained β-glucopyranosyl and α-mannopyranosyl, and rheology test had a tan δ value ≥ 0.5. The pore sizes of the hydrogels decreased by increasing the MC and BSP content, and they had better properties with respect to water loss and their swelling ratio. Evaluations in vitro and in vivo showed that all of the developed MB hydrogels have good cell viability and wound-healing properties. The M8B2 hydrogel group was found to be superior to the others from within the developed MB hydrogels. Therefore, we believe that the M8B2 hydrogel formulation has a high potential for development as a wound dressing.https://www.mdpi.com/1422-0067/23/19/12019<i>Bletilla striata</i> polysaccharidemethylcellulosehydrogelswound healingwound dressing |
| spellingShingle | Subhaini Jakfar Tzu-Chieh Lin Zhi-Yu Chen I-Hsuan Yang Basri A. Gani Diana Setya Ningsih Hendra Kusuma Chia-Tien Chang Feng-Huei Lin A Polysaccharide Isolated from the Herb <i>Bletilla striata</i> Combined with Methylcellulose to Form a Hydrogel via Self-Assembly as a Wound Dressing International Journal of Molecular Sciences <i>Bletilla striata</i> polysaccharide methylcellulose hydrogels wound healing wound dressing |
| title | A Polysaccharide Isolated from the Herb <i>Bletilla striata</i> Combined with Methylcellulose to Form a Hydrogel via Self-Assembly as a Wound Dressing |
| title_full | A Polysaccharide Isolated from the Herb <i>Bletilla striata</i> Combined with Methylcellulose to Form a Hydrogel via Self-Assembly as a Wound Dressing |
| title_fullStr | A Polysaccharide Isolated from the Herb <i>Bletilla striata</i> Combined with Methylcellulose to Form a Hydrogel via Self-Assembly as a Wound Dressing |
| title_full_unstemmed | A Polysaccharide Isolated from the Herb <i>Bletilla striata</i> Combined with Methylcellulose to Form a Hydrogel via Self-Assembly as a Wound Dressing |
| title_short | A Polysaccharide Isolated from the Herb <i>Bletilla striata</i> Combined with Methylcellulose to Form a Hydrogel via Self-Assembly as a Wound Dressing |
| title_sort | polysaccharide isolated from the herb i bletilla striata i combined with methylcellulose to form a hydrogel via self assembly as a wound dressing |
| topic | <i>Bletilla striata</i> polysaccharide methylcellulose hydrogels wound healing wound dressing |
| url | https://www.mdpi.com/1422-0067/23/19/12019 |
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