Development of a Rainbow Trout (<i>Oncorhynchus mykiss</i>) Intestinal <i>In Vitro</i> Platform for Profiling Amino Acid Digestion and Absorption of a Complete Diet
The ever-increasing number and variation of raw materials utilized to provide alternative feed formulations continues to allow for a more sustainable and flexible approach. Testing all these options <i>in vivo</i> is still the most robust and reliable manner to pick the best raw material...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/14/2278 |
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| author | Rolando Pasquariello Radmila Pavlovic Marcelo A. Chacon Federica Camin Nicole Verdile Guro Løkka Sara Panseri Massimo Faustini Amos Tandler David Peggs Trond M. Kortner Amir Bitan Tiziana A. L. Brevini Fulvio Gandolfi |
| author_facet | Rolando Pasquariello Radmila Pavlovic Marcelo A. Chacon Federica Camin Nicole Verdile Guro Løkka Sara Panseri Massimo Faustini Amos Tandler David Peggs Trond M. Kortner Amir Bitan Tiziana A. L. Brevini Fulvio Gandolfi |
| author_sort | Rolando Pasquariello |
| collection | DOAJ |
| description | The ever-increasing number and variation of raw materials utilized to provide alternative feed formulations continues to allow for a more sustainable and flexible approach. Testing all these options <i>in vivo</i> is still the most robust and reliable manner to pick the best raw material candidates, but it requires the use of large numbers of animals and is time-consuming and expensive. Therefore, we are developing an <i>in vitro</i> platform that can provide a reliable evaluation of new ingredients. The main aim of this work was to combine an <i>in vitro</i> digestion protocol of extruded, commercially relevant aquafeeds with the exposure of intestinal epithelial cells to the extracted bio-available fraction (BAF). The results show that 250,000 cells/cm<sup>2</sup> represents the optimal seeding density and that up to 50% BAF concentration for up to 24 h had no negative effects on the epithelial barrier morphology and function. It is possible to determine amino acid digestibility and bioavailability in all the experimental conditions (with and without BSA, at 25% and 50% dilution) and at all time points (0, 6, and 24 h). However, BAF concentration, the medium used for its dilution, and the length of exposure to the different epithelial cell lines can all influence the results and, therefore, must be selected according to the final aim of the experiment. |
| first_indexed | 2024-03-11T01:22:14Z |
| format | Article |
| id | doaj.art-1caf2342ef964d7c9cadf8e51cb9961e |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T01:22:14Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-1caf2342ef964d7c9cadf8e51cb9961e2023-11-18T18:00:04ZengMDPI AGAnimals2076-26152023-07-011314227810.3390/ani13142278Development of a Rainbow Trout (<i>Oncorhynchus mykiss</i>) Intestinal <i>In Vitro</i> Platform for Profiling Amino Acid Digestion and Absorption of a Complete DietRolando Pasquariello0Radmila Pavlovic1Marcelo A. Chacon2Federica Camin3Nicole Verdile4Guro Løkka5Sara Panseri6Massimo Faustini7Amos Tandler8David Peggs9Trond M. Kortner10Amir Bitan11Tiziana A. L. Brevini12Fulvio Gandolfi13Department of Agricultural and Environmental Sciences, University of Milan, 20133 Milan, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, ItalyIsrael Oceanographic and Limnological Research (IOLR), The National Center for Mariculture, Eilat 8800001, IsraelDepartment of Agricultural and Environmental Sciences, University of Milan, 20133 Milan, ItalyDepartment of Agricultural and Environmental Sciences, University of Milan, 20133 Milan, ItalyDepartment of Paraclinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), 1433 Ås, NorwayDepartment of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, ItalyIsrael Oceanographic and Limnological Research (IOLR), The National Center for Mariculture, Eilat 8800001, IsraelSkretting Aquaculture Research Centre, 4016 Stavanger, NorwayDepartment of Paraclinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), 1433 Ås, NorwayIsrael Oceanographic and Limnological Research (IOLR), The National Center for Mariculture, Eilat 8800001, IsraelDepartment of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, ItalyDepartment of Agricultural and Environmental Sciences, University of Milan, 20133 Milan, ItalyThe ever-increasing number and variation of raw materials utilized to provide alternative feed formulations continues to allow for a more sustainable and flexible approach. Testing all these options <i>in vivo</i> is still the most robust and reliable manner to pick the best raw material candidates, but it requires the use of large numbers of animals and is time-consuming and expensive. Therefore, we are developing an <i>in vitro</i> platform that can provide a reliable evaluation of new ingredients. The main aim of this work was to combine an <i>in vitro</i> digestion protocol of extruded, commercially relevant aquafeeds with the exposure of intestinal epithelial cells to the extracted bio-available fraction (BAF). The results show that 250,000 cells/cm<sup>2</sup> represents the optimal seeding density and that up to 50% BAF concentration for up to 24 h had no negative effects on the epithelial barrier morphology and function. It is possible to determine amino acid digestibility and bioavailability in all the experimental conditions (with and without BSA, at 25% and 50% dilution) and at all time points (0, 6, and 24 h). However, BAF concentration, the medium used for its dilution, and the length of exposure to the different epithelial cell lines can all influence the results and, therefore, must be selected according to the final aim of the experiment.https://www.mdpi.com/2076-2615/13/14/2278<i>in vitro</i> digestionintestinedigestibilitybioavailabilityaquaculturesustainable feed |
| spellingShingle | Rolando Pasquariello Radmila Pavlovic Marcelo A. Chacon Federica Camin Nicole Verdile Guro Løkka Sara Panseri Massimo Faustini Amos Tandler David Peggs Trond M. Kortner Amir Bitan Tiziana A. L. Brevini Fulvio Gandolfi Development of a Rainbow Trout (<i>Oncorhynchus mykiss</i>) Intestinal <i>In Vitro</i> Platform for Profiling Amino Acid Digestion and Absorption of a Complete Diet Animals <i>in vitro</i> digestion intestine digestibility bioavailability aquaculture sustainable feed |
| title | Development of a Rainbow Trout (<i>Oncorhynchus mykiss</i>) Intestinal <i>In Vitro</i> Platform for Profiling Amino Acid Digestion and Absorption of a Complete Diet |
| title_full | Development of a Rainbow Trout (<i>Oncorhynchus mykiss</i>) Intestinal <i>In Vitro</i> Platform for Profiling Amino Acid Digestion and Absorption of a Complete Diet |
| title_fullStr | Development of a Rainbow Trout (<i>Oncorhynchus mykiss</i>) Intestinal <i>In Vitro</i> Platform for Profiling Amino Acid Digestion and Absorption of a Complete Diet |
| title_full_unstemmed | Development of a Rainbow Trout (<i>Oncorhynchus mykiss</i>) Intestinal <i>In Vitro</i> Platform for Profiling Amino Acid Digestion and Absorption of a Complete Diet |
| title_short | Development of a Rainbow Trout (<i>Oncorhynchus mykiss</i>) Intestinal <i>In Vitro</i> Platform for Profiling Amino Acid Digestion and Absorption of a Complete Diet |
| title_sort | development of a rainbow trout i oncorhynchus mykiss i intestinal i in vitro i platform for profiling amino acid digestion and absorption of a complete diet |
| topic | <i>in vitro</i> digestion intestine digestibility bioavailability aquaculture sustainable feed |
| url | https://www.mdpi.com/2076-2615/13/14/2278 |
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