Calorie Restriction Curbs Proinflammation That Accompanies Arterial Aging, Preserving a Youthful Phenotype
Background Aging exponentially increases the incidence of morbidity and mortality of quintessential cardiovascular disease mainly due to arterial proinflammatory shifts at the molecular, cellular, and tissue levels within the arterial wall. Calorie restriction (CR) in rats improves arterial function...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-09-01
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| Series: | Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease |
| Subjects: | |
| Online Access: | https://www.ahajournals.org/doi/10.1161/JAHA.118.009112 |
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| author | Mingyi Wang Li Zhang Wanqu Zhu Jing Zhang Soo Hyuk Kim Yushi Wang Leng Ni Richard Telljohann Robert E. Monticone Kimberly McGraw Lijuan Liu Rafael de Cabo Edward G. Lakatta |
| author_facet | Mingyi Wang Li Zhang Wanqu Zhu Jing Zhang Soo Hyuk Kim Yushi Wang Leng Ni Richard Telljohann Robert E. Monticone Kimberly McGraw Lijuan Liu Rafael de Cabo Edward G. Lakatta |
| author_sort | Mingyi Wang |
| collection | DOAJ |
| description | Background Aging exponentially increases the incidence of morbidity and mortality of quintessential cardiovascular disease mainly due to arterial proinflammatory shifts at the molecular, cellular, and tissue levels within the arterial wall. Calorie restriction (CR) in rats improves arterial function and extends both health span and life span. How CR affects the proinflammatory landscape of molecular, cellular, and tissue phenotypic shifts within the arterial wall in rats, however, remains to be elucidated. Methods and Results Aortae were harvested from young (6‐month‐old) and old (24‐month‐old) Fischer 344 rats, fed ad libitum and a second group maintained on a 40% CR beginning at 1 month of age. Histopathologic and morphometric analysis of the arterial wall demonstrated that CR markedly reduced age‐associated intimal medial thickening, collagen deposition, and elastin fractionation/degradation within the arterial walls. Immunostaining/blotting showed that CR effectively prevented an age‐associated increase in the density of platelet‐derived growth factor, matrix metalloproteinase type II activity, and transforming growth factor beta 1 and its downstream signaling molecules, phospho‐mothers against decapentaplegic homolog‐2/3 (p‐SMAD‐2/3) in the arterial wall. In early passage cultured vascular smooth muscle cells isolated from AL and CR rat aortae, CR alleviated the age‐associated vascular smooth muscle cell phenotypic shifts, profibrogenic signaling, and migration/proliferation in response to platelet‐derived growth factor. Conclusions CR reduces matrix and cellular proinflammation associated with aging that occurs within the aortic wall and that are attributable to platelet‐derived growth factor signaling. Thus, CR reduces the platelet‐derived growth factor–associated signaling cascade, contributing to the postponement of biological aging and preservation of a more youthful aortic wall phenotype. |
| first_indexed | 2024-04-13T17:03:56Z |
| format | Article |
| id | doaj.art-1cb3608c3ec6469ea847c976e5e863a8 |
| institution | Directory Open Access Journal |
| issn | 2047-9980 |
| language | English |
| last_indexed | 2024-04-13T17:03:56Z |
| publishDate | 2018-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease |
| spelling | doaj.art-1cb3608c3ec6469ea847c976e5e863a82022-12-22T02:38:32ZengWileyJournal of the American Heart Association: Cardiovascular and Cerebrovascular Disease2047-99802018-09-0171810.1161/JAHA.118.009112Calorie Restriction Curbs Proinflammation That Accompanies Arterial Aging, Preserving a Youthful PhenotypeMingyi Wang0Li Zhang1Wanqu Zhu2Jing Zhang3Soo Hyuk Kim4Yushi Wang5Leng Ni6Richard Telljohann7Robert E. Monticone8Kimberly McGraw9Lijuan Liu10Rafael de Cabo11Edward G. Lakatta12Laboratory of Cardiovascular Science National Institute on Aging National Institutes of Health Biomedical Research Center (BRC) Baltimore MDLaboratory of Cardiovascular Science National Institute on Aging National Institutes of Health Biomedical Research Center (BRC) Baltimore MDLaboratory of Cardiovascular Science National Institute on Aging National Institutes of Health Biomedical Research Center (BRC) Baltimore MDLaboratory of Cardiovascular Science National Institute on Aging National Institutes of Health Biomedical Research Center (BRC) Baltimore MDLaboratory of Cardiovascular Science National Institute on Aging National Institutes of Health Biomedical Research Center (BRC) Baltimore MDLaboratory of Cardiovascular Science National Institute on Aging National Institutes of Health Biomedical Research Center (BRC) Baltimore MDLaboratory of Cardiovascular Science National Institute on Aging National Institutes of Health Biomedical Research Center (BRC) Baltimore MDLaboratory of Cardiovascular Science National Institute on Aging National Institutes of Health Biomedical Research Center (BRC) Baltimore MDLaboratory of Cardiovascular Science National Institute on Aging National Institutes of Health Biomedical Research Center (BRC) Baltimore MDLaboratory of Cardiovascular Science National Institute on Aging National Institutes of Health Biomedical Research Center (BRC) Baltimore MDLaboratory of Cardiovascular Science National Institute on Aging National Institutes of Health Biomedical Research Center (BRC) Baltimore MDExperimental Gerontology Section, Translational Gerontology Branch National Institute on Aging National Institutes of Health Biomedical Research Center (BRC) Baltimore MDLaboratory of Cardiovascular Science National Institute on Aging National Institutes of Health Biomedical Research Center (BRC) Baltimore MDBackground Aging exponentially increases the incidence of morbidity and mortality of quintessential cardiovascular disease mainly due to arterial proinflammatory shifts at the molecular, cellular, and tissue levels within the arterial wall. Calorie restriction (CR) in rats improves arterial function and extends both health span and life span. How CR affects the proinflammatory landscape of molecular, cellular, and tissue phenotypic shifts within the arterial wall in rats, however, remains to be elucidated. Methods and Results Aortae were harvested from young (6‐month‐old) and old (24‐month‐old) Fischer 344 rats, fed ad libitum and a second group maintained on a 40% CR beginning at 1 month of age. Histopathologic and morphometric analysis of the arterial wall demonstrated that CR markedly reduced age‐associated intimal medial thickening, collagen deposition, and elastin fractionation/degradation within the arterial walls. Immunostaining/blotting showed that CR effectively prevented an age‐associated increase in the density of platelet‐derived growth factor, matrix metalloproteinase type II activity, and transforming growth factor beta 1 and its downstream signaling molecules, phospho‐mothers against decapentaplegic homolog‐2/3 (p‐SMAD‐2/3) in the arterial wall. In early passage cultured vascular smooth muscle cells isolated from AL and CR rat aortae, CR alleviated the age‐associated vascular smooth muscle cell phenotypic shifts, profibrogenic signaling, and migration/proliferation in response to platelet‐derived growth factor. Conclusions CR reduces matrix and cellular proinflammation associated with aging that occurs within the aortic wall and that are attributable to platelet‐derived growth factor signaling. Thus, CR reduces the platelet‐derived growth factor–associated signaling cascade, contributing to the postponement of biological aging and preservation of a more youthful aortic wall phenotype.https://www.ahajournals.org/doi/10.1161/JAHA.118.009112agingarterial remodelingcalorie restrictionproinflammationratsvascular smooth muscle cells |
| spellingShingle | Mingyi Wang Li Zhang Wanqu Zhu Jing Zhang Soo Hyuk Kim Yushi Wang Leng Ni Richard Telljohann Robert E. Monticone Kimberly McGraw Lijuan Liu Rafael de Cabo Edward G. Lakatta Calorie Restriction Curbs Proinflammation That Accompanies Arterial Aging, Preserving a Youthful Phenotype Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease aging arterial remodeling calorie restriction proinflammation rats vascular smooth muscle cells |
| title | Calorie Restriction Curbs Proinflammation That Accompanies Arterial Aging, Preserving a Youthful Phenotype |
| title_full | Calorie Restriction Curbs Proinflammation That Accompanies Arterial Aging, Preserving a Youthful Phenotype |
| title_fullStr | Calorie Restriction Curbs Proinflammation That Accompanies Arterial Aging, Preserving a Youthful Phenotype |
| title_full_unstemmed | Calorie Restriction Curbs Proinflammation That Accompanies Arterial Aging, Preserving a Youthful Phenotype |
| title_short | Calorie Restriction Curbs Proinflammation That Accompanies Arterial Aging, Preserving a Youthful Phenotype |
| title_sort | calorie restriction curbs proinflammation that accompanies arterial aging preserving a youthful phenotype |
| topic | aging arterial remodeling calorie restriction proinflammation rats vascular smooth muscle cells |
| url | https://www.ahajournals.org/doi/10.1161/JAHA.118.009112 |
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