Receptor-mediated endocytosis of lysozyme in renal proximal tubules of the frog Rana temporaria
<p>The mechanism of protein reabsorption in the kidney of lower vertebrates remains insufficiently investigated in spite of raising interest to the amphibian and fish kidneys as a useful model for physiological and pathophysiological examinations. In the present study, we examined the renal tu...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2015-04-01
|
| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | http://ejh.it/index.php/ejh/article/view/2482 |
| _version_ | 1818055280489922560 |
|---|---|
| author | E.V. Seliverstova N.P. Prutskova |
| author_facet | E.V. Seliverstova N.P. Prutskova |
| author_sort | E.V. Seliverstova |
| collection | DOAJ |
| description | <p>The mechanism of protein reabsorption in the kidney of lower vertebrates remains insufficiently investigated in spite of raising interest to the amphibian and fish kidneys as a useful model for physiological and pathophysiological examinations. In the present study, we examined the renal tubular uptake and the internalization rote of lysozyme after its intravenous injection in the wintering frog <em>Rana temporaria</em> using immunohisto- and immunocytochemistry and specific markers for some endocytic compartments. The distinct expression of megalin and cubilin in the proximal tubule cells of lysozyme-injected frogs was revealed whereas kidney tissue of control animals showed no positive immunoreactivity. Lysozyme was detected in the apical endocytic compartment of the tubular cells and colocalized with clathrin 10 min after injection. After 20 min, lysozyme was located in the subapical compartment negative to clathrin (endosomes), and intracellular trafficking of lysozyme was coincided with the distribution of megalin and cubilin. However, internalized protein was retained in the endosomes and did not reach lysosomes within 30 min after treatment that may indicate the inhibition of intracellular trafficking in hibernating frogs. For the first time, we provided the evidence that lysozyme is filtered through the glomeruli and absorbed by receptor-mediated clathrin-dependent endocytosis in the frog proximal tubule cells. Thus, the protein uptake in the amphibian mesonephros is mediated by megalin and cubilin that confirms a critical role of endocytic receptors in the renal reabsorption of proteins in amphibians as in mammals.</p> |
| first_indexed | 2024-12-10T12:10:26Z |
| format | Article |
| id | doaj.art-1cb3f5292d72431095a95595cc7157b4 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-10T12:10:26Z |
| publishDate | 2015-04-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-1cb3f5292d72431095a95595cc7157b42022-12-22T01:49:23ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062015-04-0159210.4081/ejh.2015.24821451Receptor-mediated endocytosis of lysozyme in renal proximal tubules of the frog Rana temporariaE.V. Seliverstova0N.P. Prutskova1Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of SciencesSechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences<p>The mechanism of protein reabsorption in the kidney of lower vertebrates remains insufficiently investigated in spite of raising interest to the amphibian and fish kidneys as a useful model for physiological and pathophysiological examinations. In the present study, we examined the renal tubular uptake and the internalization rote of lysozyme after its intravenous injection in the wintering frog <em>Rana temporaria</em> using immunohisto- and immunocytochemistry and specific markers for some endocytic compartments. The distinct expression of megalin and cubilin in the proximal tubule cells of lysozyme-injected frogs was revealed whereas kidney tissue of control animals showed no positive immunoreactivity. Lysozyme was detected in the apical endocytic compartment of the tubular cells and colocalized with clathrin 10 min after injection. After 20 min, lysozyme was located in the subapical compartment negative to clathrin (endosomes), and intracellular trafficking of lysozyme was coincided with the distribution of megalin and cubilin. However, internalized protein was retained in the endosomes and did not reach lysosomes within 30 min after treatment that may indicate the inhibition of intracellular trafficking in hibernating frogs. For the first time, we provided the evidence that lysozyme is filtered through the glomeruli and absorbed by receptor-mediated clathrin-dependent endocytosis in the frog proximal tubule cells. Thus, the protein uptake in the amphibian mesonephros is mediated by megalin and cubilin that confirms a critical role of endocytic receptors in the renal reabsorption of proteins in amphibians as in mammals.</p>http://ejh.it/index.php/ejh/article/view/2482Endocytic receptorfrogkidneylysozymeprotein uptakeproximal tubule. |
| spellingShingle | E.V. Seliverstova N.P. Prutskova Receptor-mediated endocytosis of lysozyme in renal proximal tubules of the frog Rana temporaria European Journal of Histochemistry Endocytic receptor frog kidney lysozyme protein uptake proximal tubule. |
| title | Receptor-mediated endocytosis of lysozyme in renal proximal tubules of the frog Rana temporaria |
| title_full | Receptor-mediated endocytosis of lysozyme in renal proximal tubules of the frog Rana temporaria |
| title_fullStr | Receptor-mediated endocytosis of lysozyme in renal proximal tubules of the frog Rana temporaria |
| title_full_unstemmed | Receptor-mediated endocytosis of lysozyme in renal proximal tubules of the frog Rana temporaria |
| title_short | Receptor-mediated endocytosis of lysozyme in renal proximal tubules of the frog Rana temporaria |
| title_sort | receptor mediated endocytosis of lysozyme in renal proximal tubules of the frog rana temporaria |
| topic | Endocytic receptor frog kidney lysozyme protein uptake proximal tubule. |
| url | http://ejh.it/index.php/ejh/article/view/2482 |
| work_keys_str_mv | AT evseliverstova receptormediatedendocytosisoflysozymeinrenalproximaltubulesofthefrogranatemporaria AT npprutskova receptormediatedendocytosisoflysozymeinrenalproximaltubulesofthefrogranatemporaria |