Evaluation of Carotenoids Accumulation and Biosynthesis in Two Genotypes of Pomelo (<i>Citrus maxima</i>) during Early Fruit Development
Pomelo is rich in bioactive compounds (carotenoids, phenolics and essential oil) in the early stage of fruit development, but it is often wasted in the cultivation and management process. To gain an insight into the carotenoid metabolism pathway in pomelo, the carotenoid profiles and the expression...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-08-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/26/16/5054 |
| _version_ | 1797522681474580480 |
|---|---|
| author | Yihan Zhao Xufeng Yang Yuwei Hu Qiuming Gu Weiling Chen Jiaqi Li Xinbo Guo Yutao Liu |
| author_facet | Yihan Zhao Xufeng Yang Yuwei Hu Qiuming Gu Weiling Chen Jiaqi Li Xinbo Guo Yutao Liu |
| author_sort | Yihan Zhao |
| collection | DOAJ |
| description | Pomelo is rich in bioactive compounds (carotenoids, phenolics and essential oil) in the early stage of fruit development, but it is often wasted in the cultivation and management process. To gain an insight into the carotenoid metabolism pathway in pomelo, the carotenoid profiles and the expression patterns of carotenogenic genes were investigated in two genotypes of pomelo during early fruit development. The results showed that a higher carotenoid content was observed in honey pomelo as compared with golden pomelo, which may be related to different gene regulation mechanisms. Lutein, α-carotene, and β-carotene were the main carotenoids in pomelo young fruit, and lutein was the highest one. The accumulation of carotenoids during fruit early development in honey pomelo is related to the transcriptional regulation of <i>ZISO</i> and <i>LUT5</i>. In golden pomelo, the rate-limiting gene for carotenoids is <i>PDS</i> and <i>ZDS</i>. In addition, the expression of seven genes except <i>CRTISO</i> in honey pomelo was higher than that in golden pomelo. The results are helpful to further clarify the regulatory mechanism of carotenoid accumulation during early fruit development and provide a direction for the high-value utilization of young fruits in pomelo. |
| first_indexed | 2024-03-10T08:31:54Z |
| format | Article |
| id | doaj.art-1cb6ae4d72124f048cb9974fd8492d7f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T08:31:54Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1cb6ae4d72124f048cb9974fd8492d7f2023-11-22T08:55:58ZengMDPI AGMolecules1420-30492021-08-012616505410.3390/molecules26165054Evaluation of Carotenoids Accumulation and Biosynthesis in Two Genotypes of Pomelo (<i>Citrus maxima</i>) during Early Fruit DevelopmentYihan Zhao0Xufeng Yang1Yuwei Hu2Qiuming Gu3Weiling Chen4Jiaqi Li5Xinbo Guo6Yutao Liu7School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of South China Modern Biological Seed Industry, Ministry of Agriculture and Rural Areas, National S&T Innovation Center for Modern Agricultural Industry, Guangzhou 510520, ChinaGuangdong Lijinyou Agricultural Technology Co., Ltd., Meizhou 514743, ChinaGuangdong Lijinyou Agricultural Technology Co., Ltd., Meizhou 514743, ChinaGuangdong Lijinyou Agricultural Technology Co., Ltd., Meizhou 514743, ChinaSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510640, ChinaKey Laboratory of South China Modern Biological Seed Industry, Ministry of Agriculture and Rural Areas, National S&T Innovation Center for Modern Agricultural Industry, Guangzhou 510520, ChinaPomelo is rich in bioactive compounds (carotenoids, phenolics and essential oil) in the early stage of fruit development, but it is often wasted in the cultivation and management process. To gain an insight into the carotenoid metabolism pathway in pomelo, the carotenoid profiles and the expression patterns of carotenogenic genes were investigated in two genotypes of pomelo during early fruit development. The results showed that a higher carotenoid content was observed in honey pomelo as compared with golden pomelo, which may be related to different gene regulation mechanisms. Lutein, α-carotene, and β-carotene were the main carotenoids in pomelo young fruit, and lutein was the highest one. The accumulation of carotenoids during fruit early development in honey pomelo is related to the transcriptional regulation of <i>ZISO</i> and <i>LUT5</i>. In golden pomelo, the rate-limiting gene for carotenoids is <i>PDS</i> and <i>ZDS</i>. In addition, the expression of seven genes except <i>CRTISO</i> in honey pomelo was higher than that in golden pomelo. The results are helpful to further clarify the regulatory mechanism of carotenoid accumulation during early fruit development and provide a direction for the high-value utilization of young fruits in pomelo.https://www.mdpi.com/1420-3049/26/16/5054pomelocarotenoidsbiosynthesisfruit development |
| spellingShingle | Yihan Zhao Xufeng Yang Yuwei Hu Qiuming Gu Weiling Chen Jiaqi Li Xinbo Guo Yutao Liu Evaluation of Carotenoids Accumulation and Biosynthesis in Two Genotypes of Pomelo (<i>Citrus maxima</i>) during Early Fruit Development Molecules pomelo carotenoids biosynthesis fruit development |
| title | Evaluation of Carotenoids Accumulation and Biosynthesis in Two Genotypes of Pomelo (<i>Citrus maxima</i>) during Early Fruit Development |
| title_full | Evaluation of Carotenoids Accumulation and Biosynthesis in Two Genotypes of Pomelo (<i>Citrus maxima</i>) during Early Fruit Development |
| title_fullStr | Evaluation of Carotenoids Accumulation and Biosynthesis in Two Genotypes of Pomelo (<i>Citrus maxima</i>) during Early Fruit Development |
| title_full_unstemmed | Evaluation of Carotenoids Accumulation and Biosynthesis in Two Genotypes of Pomelo (<i>Citrus maxima</i>) during Early Fruit Development |
| title_short | Evaluation of Carotenoids Accumulation and Biosynthesis in Two Genotypes of Pomelo (<i>Citrus maxima</i>) during Early Fruit Development |
| title_sort | evaluation of carotenoids accumulation and biosynthesis in two genotypes of pomelo i citrus maxima i during early fruit development |
| topic | pomelo carotenoids biosynthesis fruit development |
| url | https://www.mdpi.com/1420-3049/26/16/5054 |
| work_keys_str_mv | AT yihanzhao evaluationofcarotenoidsaccumulationandbiosynthesisintwogenotypesofpomeloicitrusmaximaiduringearlyfruitdevelopment AT xufengyang evaluationofcarotenoidsaccumulationandbiosynthesisintwogenotypesofpomeloicitrusmaximaiduringearlyfruitdevelopment AT yuweihu evaluationofcarotenoidsaccumulationandbiosynthesisintwogenotypesofpomeloicitrusmaximaiduringearlyfruitdevelopment AT qiuminggu evaluationofcarotenoidsaccumulationandbiosynthesisintwogenotypesofpomeloicitrusmaximaiduringearlyfruitdevelopment AT weilingchen evaluationofcarotenoidsaccumulationandbiosynthesisintwogenotypesofpomeloicitrusmaximaiduringearlyfruitdevelopment AT jiaqili evaluationofcarotenoidsaccumulationandbiosynthesisintwogenotypesofpomeloicitrusmaximaiduringearlyfruitdevelopment AT xinboguo evaluationofcarotenoidsaccumulationandbiosynthesisintwogenotypesofpomeloicitrusmaximaiduringearlyfruitdevelopment AT yutaoliu evaluationofcarotenoidsaccumulationandbiosynthesisintwogenotypesofpomeloicitrusmaximaiduringearlyfruitdevelopment |