Characterization and properties of the biosurfactant produced by Candida lipolytica UCP 0988
Background: Biotechnological processes are costly, especially for the production of biosurfactants. The successful production of a biosurfactant is dependent on the development of processes using low cost raw materials. Considering the importance of the characteristics of a biosurfactant to facilita...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-01-01
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| Series: | Electronic Journal of Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345813000079 |
| _version_ | 1819148803022782464 |
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| author | Raquel Diniz Rufino Juliana Moura de Luna Galba Maria de Campos Takaki Leonie Asfora Sarubbo |
| author_facet | Raquel Diniz Rufino Juliana Moura de Luna Galba Maria de Campos Takaki Leonie Asfora Sarubbo |
| author_sort | Raquel Diniz Rufino |
| collection | DOAJ |
| description | Background: Biotechnological processes are costly, especially for the production of biosurfactants. The successful production of a biosurfactant is dependent on the development of processes using low cost raw materials. Considering the importance of the characteristics of a biosurfactant to facilitate its industrial application, the properties of the biosurfactant produced by Candida lipolytica through previously optimized medium have been established.
Results: The yeast was grown for 72 h to determine the kinetics of growth and production. The surface tension of the cell-free broth was reduced from 55 to 25 mN/m. The yield of biosurfactant was 8.0 g/l with a CMC of 0.03%. The biosurfactant was characterized as an anionic lipopeptide composed of 50% protein, 20% lipids, and 8% of carbohydrates.
Conclusions: The isolated biosurfactant showed no toxicity against different vegetable seeds: Brassica oleracea, Solanum gilo and Lactuca sativa L. and the micro-crustacean Artemia salina. The properties of the biosurfactant produced suggest its potential application in industries that require the use of effective compounds at low cost. |
| first_indexed | 2024-12-22T13:51:31Z |
| format | Article |
| id | doaj.art-1cb835f6ed7e4e338a63e6721c6cc3a6 |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-22T13:51:31Z |
| publishDate | 2014-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-1cb835f6ed7e4e338a63e6721c6cc3a62022-12-21T18:23:39ZengElsevierElectronic Journal of Biotechnology0717-34582014-01-01171343810.1016/j.ejbt.2013.12.006Characterization and properties of the biosurfactant produced by Candida lipolytica UCP 0988Raquel Diniz Rufino0Juliana Moura de Luna1Galba Maria de Campos Takaki2Leonie Asfora Sarubbo3Centro de Ciências e Tecnologia, Universidade Católica de Pernambuco, Rua do Príncipe, no 526, Bloco D, Boa Vista 50050-900, Recife, PE, BrazilCentro de Ciências e Tecnologia, Universidade Católica de Pernambuco, Rua do Príncipe, no 526, Bloco D, Boa Vista 50050-900, Recife, PE, BrazilCentro de Ciências e Tecnologia, Universidade Católica de Pernambuco, Rua do Príncipe, no 526, Bloco D, Boa Vista 50050-900, Recife, PE, BrazilCentro de Ciências e Tecnologia, Universidade Católica de Pernambuco, Rua do Príncipe, no 526, Bloco D, Boa Vista 50050-900, Recife, PE, BrazilBackground: Biotechnological processes are costly, especially for the production of biosurfactants. The successful production of a biosurfactant is dependent on the development of processes using low cost raw materials. Considering the importance of the characteristics of a biosurfactant to facilitate its industrial application, the properties of the biosurfactant produced by Candida lipolytica through previously optimized medium have been established. Results: The yeast was grown for 72 h to determine the kinetics of growth and production. The surface tension of the cell-free broth was reduced from 55 to 25 mN/m. The yield of biosurfactant was 8.0 g/l with a CMC of 0.03%. The biosurfactant was characterized as an anionic lipopeptide composed of 50% protein, 20% lipids, and 8% of carbohydrates. Conclusions: The isolated biosurfactant showed no toxicity against different vegetable seeds: Brassica oleracea, Solanum gilo and Lactuca sativa L. and the micro-crustacean Artemia salina. The properties of the biosurfactant produced suggest its potential application in industries that require the use of effective compounds at low cost.http://www.sciencedirect.com/science/article/pii/S0717345813000079BiosurfactantCandida lipolyticaLipopeptideSurface tensionToxicity |
| spellingShingle | Raquel Diniz Rufino Juliana Moura de Luna Galba Maria de Campos Takaki Leonie Asfora Sarubbo Characterization and properties of the biosurfactant produced by Candida lipolytica UCP 0988 Electronic Journal of Biotechnology Biosurfactant Candida lipolytica Lipopeptide Surface tension Toxicity |
| title | Characterization and properties of the biosurfactant produced by Candida lipolytica UCP 0988 |
| title_full | Characterization and properties of the biosurfactant produced by Candida lipolytica UCP 0988 |
| title_fullStr | Characterization and properties of the biosurfactant produced by Candida lipolytica UCP 0988 |
| title_full_unstemmed | Characterization and properties of the biosurfactant produced by Candida lipolytica UCP 0988 |
| title_short | Characterization and properties of the biosurfactant produced by Candida lipolytica UCP 0988 |
| title_sort | characterization and properties of the biosurfactant produced by candida lipolytica ucp 0988 |
| topic | Biosurfactant Candida lipolytica Lipopeptide Surface tension Toxicity |
| url | http://www.sciencedirect.com/science/article/pii/S0717345813000079 |
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