Isolation, characterization and biological activities of betulin from Acacia nilotica bark
Abstract Medicinal plants are in use of humankind since ancient and still they are playing an important role in effective and safer natural drug delivery systems. Acacia nilotica (native of Egypt) commonly known as babul belongs to family Fabaceae, widely spread in India, Sri Lanka and Sudan. Being...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13338-3 |
| _version_ | 1818207363944939520 |
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| author | Prabhjit Kaur Saroj Arora Rajbir Singh |
| author_facet | Prabhjit Kaur Saroj Arora Rajbir Singh |
| author_sort | Prabhjit Kaur |
| collection | DOAJ |
| description | Abstract Medicinal plants are in use of humankind since ancient and still they are playing an important role in effective and safer natural drug delivery systems. Acacia nilotica (native of Egypt) commonly known as babul belongs to family Fabaceae, widely spread in India, Sri Lanka and Sudan. Being a common and important plant, using in many ways from fodder (shoots and leaves to animals) to dyeing (leather coloration) to medicine (root, bark, leaves, flower, gum, pods). The present study is focused on investigating the natural chemistry and important biological activities of the plant. Employing bioassay guided fractionation coupled with TLC and column chromatography, a pure fraction named AN-10 was isolated from ethyl acetate fraction of crude methanol extract which identified as “Betulin (Lupan-3ß,28-diol)” by Liebermann-Burchard test and structure elucidation by UV–Vis, NMR and MS techniques. A battery of in vitro biological assays for antioxidant, anti-inflammatory and anticancer were performed and betulin showed excellent potential in all assays. It was found that the inhibitory potential in all assays were dose dependent manner and after a range of concentration, the activities get leveled off with no further increase in activity. |
| first_indexed | 2024-12-12T04:27:44Z |
| format | Article |
| id | doaj.art-1cb8d05836524e8385386a460e0c0f9c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T04:27:44Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1cb8d05836524e8385386a460e0c0f9c2022-12-22T00:38:09ZengNature PortfolioScientific Reports2045-23222022-06-0112111110.1038/s41598-022-13338-3Isolation, characterization and biological activities of betulin from Acacia nilotica barkPrabhjit Kaur0Saroj Arora1Rajbir Singh2Department of Botanical and Environmental Sciences, Guru Nanak Dev UniversityDepartment of Botanical and Environmental Sciences, Guru Nanak Dev UniversityDepartment of Botanical and Environmental Sciences, Guru Nanak Dev UniversityAbstract Medicinal plants are in use of humankind since ancient and still they are playing an important role in effective and safer natural drug delivery systems. Acacia nilotica (native of Egypt) commonly known as babul belongs to family Fabaceae, widely spread in India, Sri Lanka and Sudan. Being a common and important plant, using in many ways from fodder (shoots and leaves to animals) to dyeing (leather coloration) to medicine (root, bark, leaves, flower, gum, pods). The present study is focused on investigating the natural chemistry and important biological activities of the plant. Employing bioassay guided fractionation coupled with TLC and column chromatography, a pure fraction named AN-10 was isolated from ethyl acetate fraction of crude methanol extract which identified as “Betulin (Lupan-3ß,28-diol)” by Liebermann-Burchard test and structure elucidation by UV–Vis, NMR and MS techniques. A battery of in vitro biological assays for antioxidant, anti-inflammatory and anticancer were performed and betulin showed excellent potential in all assays. It was found that the inhibitory potential in all assays were dose dependent manner and after a range of concentration, the activities get leveled off with no further increase in activity.https://doi.org/10.1038/s41598-022-13338-3 |
| spellingShingle | Prabhjit Kaur Saroj Arora Rajbir Singh Isolation, characterization and biological activities of betulin from Acacia nilotica bark Scientific Reports |
| title | Isolation, characterization and biological activities of betulin from Acacia nilotica bark |
| title_full | Isolation, characterization and biological activities of betulin from Acacia nilotica bark |
| title_fullStr | Isolation, characterization and biological activities of betulin from Acacia nilotica bark |
| title_full_unstemmed | Isolation, characterization and biological activities of betulin from Acacia nilotica bark |
| title_short | Isolation, characterization and biological activities of betulin from Acacia nilotica bark |
| title_sort | isolation characterization and biological activities of betulin from acacia nilotica bark |
| url | https://doi.org/10.1038/s41598-022-13338-3 |
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