HIF-1α effects on angiogenic potential in human small cell lung carcinoma
<p>Abstract</p> <p>Background</p> <p>Hypoxia-inducible factor-1 alpha (HIF-1α) maybe an important regulatory factor for angiogenesis of small cell lung cancer (SCLC). Our study aimed to investigate the effect of HIF-1α on angiogenic potential of SCLC including two point...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-08-01
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| Series: | Journal of Experimental & Clinical Cancer Research |
| Subjects: | |
| Online Access: | http://www.jeccr.com/content/30/1/77 |
| _version_ | 1811244553465757696 |
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| author | Xia Wanli Kang Ningning Ge Wei Yu Zaicheng Chai Huiping Wan Jun Che Yun |
| author_facet | Xia Wanli Kang Ningning Ge Wei Yu Zaicheng Chai Huiping Wan Jun Che Yun |
| author_sort | Xia Wanli |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Hypoxia-inducible factor-1 alpha (HIF-1α) maybe an important regulatory factor for angiogenesis of small cell lung cancer (SCLC). Our study aimed to investigate the effect of HIF-1α on angiogenic potential of SCLC including two points: One is the effect of HIF-1α on the angiogenesis of SCLC <it>in vivo</it>. The other is the regulation of angiogenic genes by HIF-1α <it>in vitro </it>and <it>in vivo</it>.</p> <p>Methods</p> <p><it>In vivo </it>we used an alternative method to study the effect of HIF-1a on angiogenic potential of SCLC by buliding NCI-H446 cell transplantation tumor on the chick embryo chorioallantoic membrane (CAM) surface. <it>In vitro </it>we used microarray to screen out the angiogenic genes regulated by HIF-1a and tested their expression level in CAM transplantation tumor by RT-PCR and Western-blot analysis.</p> <p>Results</p> <p><it>In vivo </it>angiogenic response surrounding the SCLC transplantation tumors in chick embryo chorioallantoic membrane (CAM) was promoted after exogenous HIF-1α transduction (p < 0.05). <it>In vitro </it>the changes of angiogenic genes expression induced by HIF-1α in NCI-H446 cells were analyzed by cDNA microarray experiments. HIF-1α upregulated the expression of angiogenic genes VEGF-A, TNFAIP6, PDGFC, FN1, MMP28, MMP14 to 6.76-, 6.69-, 2.26-, 2.31-, 4.39-, 2.97- fold respectively and glycolytic genes GLUT1, GLUT2 to2.98-, 3.74- fold respectively. In addition, the expression of these angiogenic factors were also upregulated by HIF-1α in the transplantion tumors in CAM as RT-PCR and Western-blot analysis indicated.</p> <p>Conclusions</p> <p>These results indicated that HIF-1α may enhance the angiogenic potential of SCLC by regulating some angiogenic genes such as VEGF-A, MMP28 etc. Therefore, HIF-1α may be a potential target for the gene targeted therapy of SCLC.</p> |
| first_indexed | 2024-04-12T14:27:13Z |
| format | Article |
| id | doaj.art-1cb8d9fcb2cb4b37940fe9a0d35f67f9 |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-04-12T14:27:13Z |
| publishDate | 2011-08-01 |
| publisher | BMC |
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| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-1cb8d9fcb2cb4b37940fe9a0d35f67f92022-12-22T03:29:25ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662011-08-013017710.1186/1756-9966-30-77HIF-1α effects on angiogenic potential in human small cell lung carcinomaXia WanliKang NingningGe WeiYu ZaichengChai HuipingWan JunChe Yun<p>Abstract</p> <p>Background</p> <p>Hypoxia-inducible factor-1 alpha (HIF-1α) maybe an important regulatory factor for angiogenesis of small cell lung cancer (SCLC). Our study aimed to investigate the effect of HIF-1α on angiogenic potential of SCLC including two points: One is the effect of HIF-1α on the angiogenesis of SCLC <it>in vivo</it>. The other is the regulation of angiogenic genes by HIF-1α <it>in vitro </it>and <it>in vivo</it>.</p> <p>Methods</p> <p><it>In vivo </it>we used an alternative method to study the effect of HIF-1a on angiogenic potential of SCLC by buliding NCI-H446 cell transplantation tumor on the chick embryo chorioallantoic membrane (CAM) surface. <it>In vitro </it>we used microarray to screen out the angiogenic genes regulated by HIF-1a and tested their expression level in CAM transplantation tumor by RT-PCR and Western-blot analysis.</p> <p>Results</p> <p><it>In vivo </it>angiogenic response surrounding the SCLC transplantation tumors in chick embryo chorioallantoic membrane (CAM) was promoted after exogenous HIF-1α transduction (p < 0.05). <it>In vitro </it>the changes of angiogenic genes expression induced by HIF-1α in NCI-H446 cells were analyzed by cDNA microarray experiments. HIF-1α upregulated the expression of angiogenic genes VEGF-A, TNFAIP6, PDGFC, FN1, MMP28, MMP14 to 6.76-, 6.69-, 2.26-, 2.31-, 4.39-, 2.97- fold respectively and glycolytic genes GLUT1, GLUT2 to2.98-, 3.74- fold respectively. In addition, the expression of these angiogenic factors were also upregulated by HIF-1α in the transplantion tumors in CAM as RT-PCR and Western-blot analysis indicated.</p> <p>Conclusions</p> <p>These results indicated that HIF-1α may enhance the angiogenic potential of SCLC by regulating some angiogenic genes such as VEGF-A, MMP28 etc. Therefore, HIF-1α may be a potential target for the gene targeted therapy of SCLC.</p>http://www.jeccr.com/content/30/1/77SCLCHIF-1αchick embryo chorioallantoic membraneangiogenesis |
| spellingShingle | Xia Wanli Kang Ningning Ge Wei Yu Zaicheng Chai Huiping Wan Jun Che Yun HIF-1α effects on angiogenic potential in human small cell lung carcinoma Journal of Experimental & Clinical Cancer Research SCLC HIF-1α chick embryo chorioallantoic membrane angiogenesis |
| title | HIF-1α effects on angiogenic potential in human small cell lung carcinoma |
| title_full | HIF-1α effects on angiogenic potential in human small cell lung carcinoma |
| title_fullStr | HIF-1α effects on angiogenic potential in human small cell lung carcinoma |
| title_full_unstemmed | HIF-1α effects on angiogenic potential in human small cell lung carcinoma |
| title_short | HIF-1α effects on angiogenic potential in human small cell lung carcinoma |
| title_sort | hif 1α effects on angiogenic potential in human small cell lung carcinoma |
| topic | SCLC HIF-1α chick embryo chorioallantoic membrane angiogenesis |
| url | http://www.jeccr.com/content/30/1/77 |
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