Impact of Phosphorylcholine Expression on the Adherence and Invasion of <i>Streptococcus pyogenes</i> to Epithelial Cells
Phosphorylcholine (PC) is a structural component of various pathogens and is involved in bacterial adhesion via the platelet-activating factor receptor (PAF-R). In this study, we investigated how PC expression affects cell adhesion and invasion of <i>Streptococcus pyogenes</i> (<i>...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/10/3/527 |
| _version_ | 1797444688084467712 |
|---|---|
| author | Hiroyuki Iuchi Junichiro Ohori Hisahiro Matsuzaki Takeshi Tokushige Sakiko Toge Masaru Yamashita |
| author_facet | Hiroyuki Iuchi Junichiro Ohori Hisahiro Matsuzaki Takeshi Tokushige Sakiko Toge Masaru Yamashita |
| author_sort | Hiroyuki Iuchi |
| collection | DOAJ |
| description | Phosphorylcholine (PC) is a structural component of various pathogens and is involved in bacterial adhesion via the platelet-activating factor receptor (PAF-R). In this study, we investigated how PC expression affects cell adhesion and invasion of <i>Streptococcus pyogenes</i> (<i>S. pyogenes</i>). Eight clinical strains of <i>S. pyogenes</i> were cultured, and PC expression was measured using fluorescence-activated cell sorting. Bacterial adherence and invasion were examined using Detroit 562 cells. An anti-PC-specific monoclonal antibody (TEPC-15) was used to inhibit bacterial PC, and a PAF-R antagonist (ABT-491) was used to inhibit cellular PAF-R. The <i>emm</i> gene was amplified by the polymerase chain reaction with the standard primers. The level of PC expressed on the <i>S. pyogenes</i> surfaces differed in each strain and differed even in the same <i>emm</i> genotype. Adherence assay experiments showed a significant negative correlation between TEPC-15 and ABT-491 inhibitory effects and PC expression in <i>S. pyogenes</i>. Similarly, intracellular invasion assay experiments showed a significant negative correlation between TEPC-15 and ABT-491 inhibitory effects and PC expression in <i>S. pyogenes</i>. This study suggests that <i>S. pyogenes</i> is involved in cell adhesion and invasion by PC. |
| first_indexed | 2024-03-09T13:16:10Z |
| format | Article |
| id | doaj.art-1cb91e7e735645f7a34829ddc2c59760 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T13:16:10Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-1cb91e7e735645f7a34829ddc2c597602023-11-30T21:35:32ZengMDPI AGMicroorganisms2076-26072022-02-0110352710.3390/microorganisms10030527Impact of Phosphorylcholine Expression on the Adherence and Invasion of <i>Streptococcus pyogenes</i> to Epithelial CellsHiroyuki Iuchi0Junichiro Ohori1Hisahiro Matsuzaki2Takeshi Tokushige3Sakiko Toge4Masaru Yamashita5Department of Otolaryngology, Head and Neck Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, JapanDepartment of Otolaryngology, Head and Neck Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, JapanDepartment of Otolaryngology, Head and Neck Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, JapanDepartment of Otolaryngology, Head and Neck Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, JapanDepartment of Otolaryngology, Head and Neck Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, JapanDepartment of Otolaryngology, Head and Neck Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, JapanPhosphorylcholine (PC) is a structural component of various pathogens and is involved in bacterial adhesion via the platelet-activating factor receptor (PAF-R). In this study, we investigated how PC expression affects cell adhesion and invasion of <i>Streptococcus pyogenes</i> (<i>S. pyogenes</i>). Eight clinical strains of <i>S. pyogenes</i> were cultured, and PC expression was measured using fluorescence-activated cell sorting. Bacterial adherence and invasion were examined using Detroit 562 cells. An anti-PC-specific monoclonal antibody (TEPC-15) was used to inhibit bacterial PC, and a PAF-R antagonist (ABT-491) was used to inhibit cellular PAF-R. The <i>emm</i> gene was amplified by the polymerase chain reaction with the standard primers. The level of PC expressed on the <i>S. pyogenes</i> surfaces differed in each strain and differed even in the same <i>emm</i> genotype. Adherence assay experiments showed a significant negative correlation between TEPC-15 and ABT-491 inhibitory effects and PC expression in <i>S. pyogenes</i>. Similarly, intracellular invasion assay experiments showed a significant negative correlation between TEPC-15 and ABT-491 inhibitory effects and PC expression in <i>S. pyogenes</i>. This study suggests that <i>S. pyogenes</i> is involved in cell adhesion and invasion by PC.https://www.mdpi.com/2076-2607/10/3/527phosphorylcholine<i>Streptococcus pyogenes</i>PAF-R<i>emm</i> genotype |
| spellingShingle | Hiroyuki Iuchi Junichiro Ohori Hisahiro Matsuzaki Takeshi Tokushige Sakiko Toge Masaru Yamashita Impact of Phosphorylcholine Expression on the Adherence and Invasion of <i>Streptococcus pyogenes</i> to Epithelial Cells Microorganisms phosphorylcholine <i>Streptococcus pyogenes</i> PAF-R <i>emm</i> genotype |
| title | Impact of Phosphorylcholine Expression on the Adherence and Invasion of <i>Streptococcus pyogenes</i> to Epithelial Cells |
| title_full | Impact of Phosphorylcholine Expression on the Adherence and Invasion of <i>Streptococcus pyogenes</i> to Epithelial Cells |
| title_fullStr | Impact of Phosphorylcholine Expression on the Adherence and Invasion of <i>Streptococcus pyogenes</i> to Epithelial Cells |
| title_full_unstemmed | Impact of Phosphorylcholine Expression on the Adherence and Invasion of <i>Streptococcus pyogenes</i> to Epithelial Cells |
| title_short | Impact of Phosphorylcholine Expression on the Adherence and Invasion of <i>Streptococcus pyogenes</i> to Epithelial Cells |
| title_sort | impact of phosphorylcholine expression on the adherence and invasion of i streptococcus pyogenes i to epithelial cells |
| topic | phosphorylcholine <i>Streptococcus pyogenes</i> PAF-R <i>emm</i> genotype |
| url | https://www.mdpi.com/2076-2607/10/3/527 |
| work_keys_str_mv | AT hiroyukiiuchi impactofphosphorylcholineexpressionontheadherenceandinvasionofistreptococcuspyogenesitoepithelialcells AT junichiroohori impactofphosphorylcholineexpressionontheadherenceandinvasionofistreptococcuspyogenesitoepithelialcells AT hisahiromatsuzaki impactofphosphorylcholineexpressionontheadherenceandinvasionofistreptococcuspyogenesitoepithelialcells AT takeshitokushige impactofphosphorylcholineexpressionontheadherenceandinvasionofistreptococcuspyogenesitoepithelialcells AT sakikotoge impactofphosphorylcholineexpressionontheadherenceandinvasionofistreptococcuspyogenesitoepithelialcells AT masaruyamashita impactofphosphorylcholineexpressionontheadherenceandinvasionofistreptococcuspyogenesitoepithelialcells |