Silver Nanoparticles Derived by <i>Artemisia</i> <i>arborescens</i> Reveal Anticancer and Apoptosis-Inducing Effects
The fight against cancer is one of the main challenges for medical research. Recently, nanotechnology has made significant progress, providing possibilities for developing innovative nanomaterials to overcome the common limitations of current therapies. In this context, silver nanoparticles (AgNPs)...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-08-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/16/8621 |
| _version_ | 1797523549048537088 |
|---|---|
| author | Valentina Bordoni Luca Sanna Weidong Lyu Elisabetta Avitabile Stefano Zoroddu Serenella Medici David J. Kelvin Luigi Bagella |
| author_facet | Valentina Bordoni Luca Sanna Weidong Lyu Elisabetta Avitabile Stefano Zoroddu Serenella Medici David J. Kelvin Luigi Bagella |
| author_sort | Valentina Bordoni |
| collection | DOAJ |
| description | The fight against cancer is one of the main challenges for medical research. Recently, nanotechnology has made significant progress, providing possibilities for developing innovative nanomaterials to overcome the common limitations of current therapies. In this context, silver nanoparticles (AgNPs) represent a promising nano-tool able to offer interesting applications for cancer research. Following this path, we combined the silver proprieties with <i>Artemisia</i> <i>arborescens</i> characteristics, producing novel nanoparticles called <i>Artemisia–AgNP</i>s. A “green” synthesis method was performed to produce <i>Artemisia–AgNP</i>s, using <i>Artemisia</i> <i>arborescens</i> extracts. This kind of photosynthesis is an eco-friendly, inexpensive, and fast approach. Moreover, the bioorganic molecules of plant extracts improved the biocompatibility and efficacy of <i>Artemisia–AgNP</i>s. The <i>Artemisia–AgNP</i>s were fully characterized and tested to compare their effects on various cancer cell lines, in particular HeLa and MCF-7. <i>Artemisia–AgNP</i>s treatment showed dose-dependent growth inhibition of cancer cells. Moreover, we evaluated their impact on the cell cycle, observing a G1 arrest mediated by <i>Artemisia–AgNP</i>s treatment. Using a clonogenic assay after treatment, we observed a complete lack of cell colonies, which demonstrated cell reproducibility death. To have a broader overview on gene expression impact, we performed RNA-sequencing, which demonstrated the potential of <i>Artemisia–AgNP</i>s as a suitable candidate tool in cancer research. |
| first_indexed | 2024-03-10T08:44:33Z |
| format | Article |
| id | doaj.art-1cbb1024cf254e04bc483b9f4e02eac7 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:44:33Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1cbb1024cf254e04bc483b9f4e02eac72023-11-22T07:57:57ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012216862110.3390/ijms22168621Silver Nanoparticles Derived by <i>Artemisia</i> <i>arborescens</i> Reveal Anticancer and Apoptosis-Inducing EffectsValentina Bordoni0Luca Sanna1Weidong Lyu2Elisabetta Avitabile3Stefano Zoroddu4Serenella Medici5David J. Kelvin6Luigi Bagella7Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/b, 07100 Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, Viale San Pietro 43/b, 07100 Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, Viale San Pietro 43/b, 07100 Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, Viale San Pietro 43/b, 07100 Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, Viale San Pietro 43/b, 07100 Sassari, ItalyDepartment of Chemistry and Pharmacy, University of Sassari, Via Muroni 23, 07100 Sassari, ItalyDivision of Immunology, International Institute of Infection and Immunity, Shantou University Medical College, Shantou 515011, ChinaDepartment of Biomedical Sciences, University of Sassari, Viale San Pietro 43/b, 07100 Sassari, ItalyThe fight against cancer is one of the main challenges for medical research. Recently, nanotechnology has made significant progress, providing possibilities for developing innovative nanomaterials to overcome the common limitations of current therapies. In this context, silver nanoparticles (AgNPs) represent a promising nano-tool able to offer interesting applications for cancer research. Following this path, we combined the silver proprieties with <i>Artemisia</i> <i>arborescens</i> characteristics, producing novel nanoparticles called <i>Artemisia–AgNP</i>s. A “green” synthesis method was performed to produce <i>Artemisia–AgNP</i>s, using <i>Artemisia</i> <i>arborescens</i> extracts. This kind of photosynthesis is an eco-friendly, inexpensive, and fast approach. Moreover, the bioorganic molecules of plant extracts improved the biocompatibility and efficacy of <i>Artemisia–AgNP</i>s. The <i>Artemisia–AgNP</i>s were fully characterized and tested to compare their effects on various cancer cell lines, in particular HeLa and MCF-7. <i>Artemisia–AgNP</i>s treatment showed dose-dependent growth inhibition of cancer cells. Moreover, we evaluated their impact on the cell cycle, observing a G1 arrest mediated by <i>Artemisia–AgNP</i>s treatment. Using a clonogenic assay after treatment, we observed a complete lack of cell colonies, which demonstrated cell reproducibility death. To have a broader overview on gene expression impact, we performed RNA-sequencing, which demonstrated the potential of <i>Artemisia–AgNP</i>s as a suitable candidate tool in cancer research.https://www.mdpi.com/1422-0067/22/16/8621cancer researchnanotechnologysilver nanoparticles<i>Artemisia arborescens</i>RNA-seq |
| spellingShingle | Valentina Bordoni Luca Sanna Weidong Lyu Elisabetta Avitabile Stefano Zoroddu Serenella Medici David J. Kelvin Luigi Bagella Silver Nanoparticles Derived by <i>Artemisia</i> <i>arborescens</i> Reveal Anticancer and Apoptosis-Inducing Effects International Journal of Molecular Sciences cancer research nanotechnology silver nanoparticles <i>Artemisia arborescens</i> RNA-seq |
| title | Silver Nanoparticles Derived by <i>Artemisia</i> <i>arborescens</i> Reveal Anticancer and Apoptosis-Inducing Effects |
| title_full | Silver Nanoparticles Derived by <i>Artemisia</i> <i>arborescens</i> Reveal Anticancer and Apoptosis-Inducing Effects |
| title_fullStr | Silver Nanoparticles Derived by <i>Artemisia</i> <i>arborescens</i> Reveal Anticancer and Apoptosis-Inducing Effects |
| title_full_unstemmed | Silver Nanoparticles Derived by <i>Artemisia</i> <i>arborescens</i> Reveal Anticancer and Apoptosis-Inducing Effects |
| title_short | Silver Nanoparticles Derived by <i>Artemisia</i> <i>arborescens</i> Reveal Anticancer and Apoptosis-Inducing Effects |
| title_sort | silver nanoparticles derived by i artemisia i i arborescens i reveal anticancer and apoptosis inducing effects |
| topic | cancer research nanotechnology silver nanoparticles <i>Artemisia arborescens</i> RNA-seq |
| url | https://www.mdpi.com/1422-0067/22/16/8621 |
| work_keys_str_mv | AT valentinabordoni silvernanoparticlesderivedbyiartemisiaiiarborescensirevealanticancerandapoptosisinducingeffects AT lucasanna silvernanoparticlesderivedbyiartemisiaiiarborescensirevealanticancerandapoptosisinducingeffects AT weidonglyu silvernanoparticlesderivedbyiartemisiaiiarborescensirevealanticancerandapoptosisinducingeffects AT elisabettaavitabile silvernanoparticlesderivedbyiartemisiaiiarborescensirevealanticancerandapoptosisinducingeffects AT stefanozoroddu silvernanoparticlesderivedbyiartemisiaiiarborescensirevealanticancerandapoptosisinducingeffects AT serenellamedici silvernanoparticlesderivedbyiartemisiaiiarborescensirevealanticancerandapoptosisinducingeffects AT davidjkelvin silvernanoparticlesderivedbyiartemisiaiiarborescensirevealanticancerandapoptosisinducingeffects AT luigibagella silvernanoparticlesderivedbyiartemisiaiiarborescensirevealanticancerandapoptosisinducingeffects |