Cross-Over Pathogenic Bacteria Detected in Infected Tomatoes (<i>Solanum lycopersicum</i> L.) and Peppers (<i>Capsicum annuum</i> L.) in Bulgaria
The ability of certain human pathogens to adapt to plants without losing their virulence toward people is a major concern today. Thus, the aim of the present work was the investigation of the presence of cross-over pathogenic bacteria in infected tomato and pepper plants. The objects of the study we...
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MDPI AG
2022-12-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/12/1507 |
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| author | Yoana Kizheva Georgi Georgiev Deyan Donchev Melani Dimitrova Maria Pandova Iliyana Rasheva Petya Hristova |
| author_facet | Yoana Kizheva Georgi Georgiev Deyan Donchev Melani Dimitrova Maria Pandova Iliyana Rasheva Petya Hristova |
| author_sort | Yoana Kizheva |
| collection | DOAJ |
| description | The ability of certain human pathogens to adapt to plants without losing their virulence toward people is a major concern today. Thus, the aim of the present work was the investigation of the presence of cross-over pathogenic bacteria in infected tomato and pepper plants. The objects of the study were 21 samples from seven different parts of the plants and three from tomato rhizosphere. In total, 26 strains were isolated, identified by MALDI-TOF, and phenotypically characterized. The PCR amplification of the <i>rpo</i>B gene was applied as an approach for the rapid detection of cross-over pathogens in plant samples. A great bacterial diversity was revealed from tomato samples as nine species were identified <i>(Leclercia adecarboxylata</i>, <i>Pseudesherichia vulneris</i>, <i>Enterobacter cancerogenus</i>, <i>Enterobacter cloacae</i>, <i>Enterobacter bugandensis</i>, <i>Acinetobacter calcoaceticus</i>, <i>Pantoea agglomerans</i>, <i>Pantoea ananatis</i>, and <i>Pectobacterium carotovorum</i>). Polymicrobial contaminations were observed in samples T2 (tomato flower) and T10 (tomato fruit). Five species were identified from pepper samples (<i>P. agglomerans</i>, <i>L. adecarboxylata</i>, <i>Pseudomonas</i> sp., <i>Pseudomonas putida</i>, and <i>Enterococcus</i> sp.). Antibiotic resistance patterns were assigned in accordance with EFSA recommendations. All isolates showed varying resistance to the tested antibiotics. The genetic basis for the phenotypic antibiotic resistance was not revealed. No genes for the virulence factors were found among the population. To our knowledge, this is the first overall investigation of tomato and pepper cross-over pathogenic bacterial populations in Bulgaria. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T15:59:02Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-1cbb657abfc34d1a876cc795e212d9df2023-11-24T17:13:55ZengMDPI AGPathogens2076-08172022-12-011112150710.3390/pathogens11121507Cross-Over Pathogenic Bacteria Detected in Infected Tomatoes (<i>Solanum lycopersicum</i> L.) and Peppers (<i>Capsicum annuum</i> L.) in BulgariaYoana Kizheva0Georgi Georgiev1Deyan Donchev2Melani Dimitrova3Maria Pandova4Iliyana Rasheva5Petya Hristova6Department of General and Industrial Microbiology, Faculty of Biology, Sofia University, 1504 Sofia, BulgariaDepartment of General and Industrial Microbiology, Faculty of Biology, Sofia University, 1504 Sofia, BulgariaDepartment of General and Industrial Microbiology, Faculty of Biology, Sofia University, 1504 Sofia, BulgariaDepartment of General and Industrial Microbiology, Faculty of Biology, Sofia University, 1504 Sofia, BulgariaDepartment of General and Industrial Microbiology, Faculty of Biology, Sofia University, 1504 Sofia, BulgariaDepartment of General and Industrial Microbiology, Faculty of Biology, Sofia University, 1504 Sofia, BulgariaDepartment of General and Industrial Microbiology, Faculty of Biology, Sofia University, 1504 Sofia, BulgariaThe ability of certain human pathogens to adapt to plants without losing their virulence toward people is a major concern today. Thus, the aim of the present work was the investigation of the presence of cross-over pathogenic bacteria in infected tomato and pepper plants. The objects of the study were 21 samples from seven different parts of the plants and three from tomato rhizosphere. In total, 26 strains were isolated, identified by MALDI-TOF, and phenotypically characterized. The PCR amplification of the <i>rpo</i>B gene was applied as an approach for the rapid detection of cross-over pathogens in plant samples. A great bacterial diversity was revealed from tomato samples as nine species were identified <i>(Leclercia adecarboxylata</i>, <i>Pseudesherichia vulneris</i>, <i>Enterobacter cancerogenus</i>, <i>Enterobacter cloacae</i>, <i>Enterobacter bugandensis</i>, <i>Acinetobacter calcoaceticus</i>, <i>Pantoea agglomerans</i>, <i>Pantoea ananatis</i>, and <i>Pectobacterium carotovorum</i>). Polymicrobial contaminations were observed in samples T2 (tomato flower) and T10 (tomato fruit). Five species were identified from pepper samples (<i>P. agglomerans</i>, <i>L. adecarboxylata</i>, <i>Pseudomonas</i> sp., <i>Pseudomonas putida</i>, and <i>Enterococcus</i> sp.). Antibiotic resistance patterns were assigned in accordance with EFSA recommendations. All isolates showed varying resistance to the tested antibiotics. The genetic basis for the phenotypic antibiotic resistance was not revealed. No genes for the virulence factors were found among the population. To our knowledge, this is the first overall investigation of tomato and pepper cross-over pathogenic bacterial populations in Bulgaria.https://www.mdpi.com/2076-0817/11/12/1507cross-over pathogensfoodborne infections<i>Solanum lycopersicum</i> L.<i>Capsicum annuum</i> L.antibiotic resistance |
| spellingShingle | Yoana Kizheva Georgi Georgiev Deyan Donchev Melani Dimitrova Maria Pandova Iliyana Rasheva Petya Hristova Cross-Over Pathogenic Bacteria Detected in Infected Tomatoes (<i>Solanum lycopersicum</i> L.) and Peppers (<i>Capsicum annuum</i> L.) in Bulgaria Pathogens cross-over pathogens foodborne infections <i>Solanum lycopersicum</i> L. <i>Capsicum annuum</i> L. antibiotic resistance |
| title | Cross-Over Pathogenic Bacteria Detected in Infected Tomatoes (<i>Solanum lycopersicum</i> L.) and Peppers (<i>Capsicum annuum</i> L.) in Bulgaria |
| title_full | Cross-Over Pathogenic Bacteria Detected in Infected Tomatoes (<i>Solanum lycopersicum</i> L.) and Peppers (<i>Capsicum annuum</i> L.) in Bulgaria |
| title_fullStr | Cross-Over Pathogenic Bacteria Detected in Infected Tomatoes (<i>Solanum lycopersicum</i> L.) and Peppers (<i>Capsicum annuum</i> L.) in Bulgaria |
| title_full_unstemmed | Cross-Over Pathogenic Bacteria Detected in Infected Tomatoes (<i>Solanum lycopersicum</i> L.) and Peppers (<i>Capsicum annuum</i> L.) in Bulgaria |
| title_short | Cross-Over Pathogenic Bacteria Detected in Infected Tomatoes (<i>Solanum lycopersicum</i> L.) and Peppers (<i>Capsicum annuum</i> L.) in Bulgaria |
| title_sort | cross over pathogenic bacteria detected in infected tomatoes i solanum lycopersicum i l and peppers i capsicum annuum i l in bulgaria |
| topic | cross-over pathogens foodborne infections <i>Solanum lycopersicum</i> L. <i>Capsicum annuum</i> L. antibiotic resistance |
| url | https://www.mdpi.com/2076-0817/11/12/1507 |
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