Smartphone-Based High-Throughput Fluorimetric Assay for Histidine Quantification in Human Urine Using 96-Well Plates

Smartphone-Based High-Throughput Fluorimetric Assay for Histidine Quantification in Human Urine Using 96-Well Plates

A high-throughput fluorimetric assay for histidine was developed, using a 96-well plates platform. The analyte reacts selectively with <i>o</i>-phthalaldehyde under mild alkaline conditions to form a stable derivative. Instrumental-free detection was carried out using a smartphone after...

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Bibliographic Details
Main Authors: Dimitrios Baltzis, George Z. Tsogas, Constantinos K. Zacharis, Paraskevas D. Tzanavaras
Format: Article
Language:English
Published: MDPI AG 2023-08-01
Series:Molecules
Subjects:
histidine
96-well plates
high-throughput assay
human urine
fluorimetry
smartphone detection
Online Access:https://www.mdpi.com/1420-3049/28/17/6205

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