DNA fingerprinting analyses of M tuberculosis-complex isolates from the Free State, South Africa, as part of a multidisciplinary study
The objective of this study was to serve as a complement to socio-economic analyses of TB patients in a DOTS system, providing microbiological data and documenting the TB population dynamics. Sputum samples were collected from smear-positive TB patients in the Goldfields, Thaba Nchu and Qwaqwa area...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of the Free State
2005-01-01
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| Series: | Acta Academica |
| Online Access: | http://196.255.246.28/index.php/aa/article/view/1044 |
| _version_ | 1797258139141144576 |
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| author | Anneke van der Spoel-van Dijk Zac Mokhethi Macala Khumalo Choala Shamputa Zacheus Matebesi Dingie van Rensburg Francoise Portaels Leen Rigouts |
| author_facet | Anneke van der Spoel-van Dijk Zac Mokhethi Macala Khumalo Choala Shamputa Zacheus Matebesi Dingie van Rensburg Francoise Portaels Leen Rigouts |
| author_sort | Anneke van der Spoel-van Dijk |
| collection | DOAJ |
| description |
The objective of this study was to serve as a complement to socio-economic analyses of TB patients in a DOTS system, providing microbiological data and documenting the TB population dynamics. Sputum samples were collected from smear-positive TB patients in the Goldfields, Thaba Nchu and Qwaqwa areas. Laboratory analyses comprised the culturing of Mycobacterium tuberculosis isolates and DNA fingerprinting. The primary aim was hampered by problems encountered during specimen sampling, inadequate resources, and a low culture-positivity rate. Nevertheless, the fingerprinting data of a random sample showed a heterogenous TB population, suggesting that reactivation might be an important factor in the area studied. Clustering was the highest in the mining area. Preliminary data from serial isolates also detected possible re-infection during treatment or initial mixed infections in five of the eleven patients.
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| first_indexed | 2024-04-24T22:48:47Z |
| format | Article |
| id | doaj.art-1cbd348bca0d4467b7b2d6180ad73109 |
| institution | Directory Open Access Journal |
| issn | 0587-2405 2415-0479 |
| language | English |
| last_indexed | 2024-04-24T22:48:47Z |
| publishDate | 2005-01-01 |
| publisher | University of the Free State |
| record_format | Article |
| series | Acta Academica |
| spelling | doaj.art-1cbd348bca0d4467b7b2d6180ad731092024-03-18T11:07:40ZengUniversity of the Free StateActa Academica0587-24052415-04792005-01-01110.38140/aa.v0i1.1044DNA fingerprinting analyses of M tuberculosis-complex isolates from the Free State, South Africa, as part of a multidisciplinary studyAnneke van der Spoel-van Dijk0Zac Mokhethi1Macala Khumalo2Choala Shamputa3Zacheus Matebesi4Dingie van Rensburg5Francoise Portaels6Leen Rigouts7University of the Free StateUniversity of the Free StateUniversity of the Free StateInstitute of Tropical Medicine, AntwerpUniversity of the Free StateUniversity of the Free StateInstitute of Tropical Medicine, AntwerpInstitute of Tropical Medicine, Antwerp The objective of this study was to serve as a complement to socio-economic analyses of TB patients in a DOTS system, providing microbiological data and documenting the TB population dynamics. Sputum samples were collected from smear-positive TB patients in the Goldfields, Thaba Nchu and Qwaqwa areas. Laboratory analyses comprised the culturing of Mycobacterium tuberculosis isolates and DNA fingerprinting. The primary aim was hampered by problems encountered during specimen sampling, inadequate resources, and a low culture-positivity rate. Nevertheless, the fingerprinting data of a random sample showed a heterogenous TB population, suggesting that reactivation might be an important factor in the area studied. Clustering was the highest in the mining area. Preliminary data from serial isolates also detected possible re-infection during treatment or initial mixed infections in five of the eleven patients. http://196.255.246.28/index.php/aa/article/view/1044 |
| spellingShingle | Anneke van der Spoel-van Dijk Zac Mokhethi Macala Khumalo Choala Shamputa Zacheus Matebesi Dingie van Rensburg Francoise Portaels Leen Rigouts DNA fingerprinting analyses of M tuberculosis-complex isolates from the Free State, South Africa, as part of a multidisciplinary study Acta Academica |
| title | DNA fingerprinting analyses of M tuberculosis-complex isolates from the Free State, South Africa, as part of a multidisciplinary study |
| title_full | DNA fingerprinting analyses of M tuberculosis-complex isolates from the Free State, South Africa, as part of a multidisciplinary study |
| title_fullStr | DNA fingerprinting analyses of M tuberculosis-complex isolates from the Free State, South Africa, as part of a multidisciplinary study |
| title_full_unstemmed | DNA fingerprinting analyses of M tuberculosis-complex isolates from the Free State, South Africa, as part of a multidisciplinary study |
| title_short | DNA fingerprinting analyses of M tuberculosis-complex isolates from the Free State, South Africa, as part of a multidisciplinary study |
| title_sort | dna fingerprinting analyses of m tuberculosis complex isolates from the free state south africa as part of a multidisciplinary study |
| url | http://196.255.246.28/index.php/aa/article/view/1044 |
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