Second Generation Ethanol Production from Brewers’ Spent Grain
Ethanol production from lignocellulosic biomasses raises a global interest because it represents a good alternative to petroleum-derived energies and reduces the food versus fuel conflict generated by first generation ethanol. In this study, alkaline-acid pretreated brewers’ spent grain (BSG) was ev...
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MDPI AG
2015-03-01
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| Series: | Energies |
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| Online Access: | http://www.mdpi.com/1996-1073/8/4/2575 |
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| author | Rossana Liguori Carlos Ricardo Soccol Luciana Porto de Souza Vandenberghe Adenise Lorenci Woiciechowski Vincenza Faraco |
| author_facet | Rossana Liguori Carlos Ricardo Soccol Luciana Porto de Souza Vandenberghe Adenise Lorenci Woiciechowski Vincenza Faraco |
| author_sort | Rossana Liguori |
| collection | DOAJ |
| description | Ethanol production from lignocellulosic biomasses raises a global interest because it represents a good alternative to petroleum-derived energies and reduces the food versus fuel conflict generated by first generation ethanol. In this study, alkaline-acid pretreated brewers’ spent grain (BSG) was evaluated for ethanol production after enzymatic hydrolysis with commercial enzymes. The obtained hydrolysate containing a glucose concentration of 75 g/L was adopted, after dilution up to 50 g/L, for fermentation by the strain Saccharomyces cerevisiae NRRL YB 2293 selected as the best producer among five ethanologenic microorganims. When the hydrolysate was supplemented with yeast extract, 12.79 g/L of ethanol, corresponding to 0.28 g of ethanol per grams of glucose consumed (55% efficiency), was obtained within 24 h, while in the non-supplemented hydrolysate, a similar concentration was reached within 48 h. The volumetric productivity increased from 0.25 g/L·h in the un-supplemented hydrolysate to 0.53 g/L h in the yeast extract supplemented hydrolysate. In conclusion, the strain S. cerevisiae NRRL YB 2293 was shown able to produce ethanol from BSG. Although an equal amount of ethanol was reached in both BSG hydrolysate media, the nitrogen source supplementation reduced the ethanol fermentation time and promoted glucose uptake and cell growth. |
| first_indexed | 2024-04-11T13:41:38Z |
| format | Article |
| id | doaj.art-1cc1f30ab46642848dcad46dcb5639a9 |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-04-11T13:41:38Z |
| publishDate | 2015-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-1cc1f30ab46642848dcad46dcb5639a92022-12-22T04:21:13ZengMDPI AGEnergies1996-10732015-03-01842575258610.3390/en8042575en8042575Second Generation Ethanol Production from Brewers’ Spent GrainRossana Liguori0Carlos Ricardo Soccol1Luciana Porto de Souza Vandenberghe2Adenise Lorenci Woiciechowski3Vincenza Faraco4Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, via Cintia, 480126 Naples, ItalyDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue, 210, 81531-980 Curitiba, BrazilDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue, 210, 81531-980 Curitiba, BrazilDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue, 210, 81531-980 Curitiba, BrazilDepartment of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, via Cintia, 480126 Naples, ItalyEthanol production from lignocellulosic biomasses raises a global interest because it represents a good alternative to petroleum-derived energies and reduces the food versus fuel conflict generated by first generation ethanol. In this study, alkaline-acid pretreated brewers’ spent grain (BSG) was evaluated for ethanol production after enzymatic hydrolysis with commercial enzymes. The obtained hydrolysate containing a glucose concentration of 75 g/L was adopted, after dilution up to 50 g/L, for fermentation by the strain Saccharomyces cerevisiae NRRL YB 2293 selected as the best producer among five ethanologenic microorganims. When the hydrolysate was supplemented with yeast extract, 12.79 g/L of ethanol, corresponding to 0.28 g of ethanol per grams of glucose consumed (55% efficiency), was obtained within 24 h, while in the non-supplemented hydrolysate, a similar concentration was reached within 48 h. The volumetric productivity increased from 0.25 g/L·h in the un-supplemented hydrolysate to 0.53 g/L h in the yeast extract supplemented hydrolysate. In conclusion, the strain S. cerevisiae NRRL YB 2293 was shown able to produce ethanol from BSG. Although an equal amount of ethanol was reached in both BSG hydrolysate media, the nitrogen source supplementation reduced the ethanol fermentation time and promoted glucose uptake and cell growth.http://www.mdpi.com/1996-1073/8/4/2575brewers’ spent grainlignocellulosic conversionsecond generation bioethanolenzymatic saccharificationethanologenic microorganisms |
| spellingShingle | Rossana Liguori Carlos Ricardo Soccol Luciana Porto de Souza Vandenberghe Adenise Lorenci Woiciechowski Vincenza Faraco Second Generation Ethanol Production from Brewers’ Spent Grain Energies brewers’ spent grain lignocellulosic conversion second generation bioethanol enzymatic saccharification ethanologenic microorganisms |
| title | Second Generation Ethanol Production from Brewers’ Spent Grain |
| title_full | Second Generation Ethanol Production from Brewers’ Spent Grain |
| title_fullStr | Second Generation Ethanol Production from Brewers’ Spent Grain |
| title_full_unstemmed | Second Generation Ethanol Production from Brewers’ Spent Grain |
| title_short | Second Generation Ethanol Production from Brewers’ Spent Grain |
| title_sort | second generation ethanol production from brewers spent grain |
| topic | brewers’ spent grain lignocellulosic conversion second generation bioethanol enzymatic saccharification ethanologenic microorganisms |
| url | http://www.mdpi.com/1996-1073/8/4/2575 |
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